A method for the permeabilization of live Drosophila melanogaster larvae to small molecules and cryoprotectants
The larvae of Drosophila melanogaster is a model organism widely used to study the muscular and nervous systems. Drosophila larvae are surrounded by a waxy cuticle that prevents permeation by most substances. Here we develop a method to remove this layer, rendering the larvae permeable to small mole...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2020-10-01
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| Series: | Fly |
| Subjects: | |
| Online Access: | http://dx.doi.org/10.1080/19336934.2020.1724051 |
| _version_ | 1797678396632727552 |
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| author | Alex Murray Daniel Palmer Daimark Bennett Venkata Dwarampudi João Pedro de Magalhães |
| author_facet | Alex Murray Daniel Palmer Daimark Bennett Venkata Dwarampudi João Pedro de Magalhães |
| author_sort | Alex Murray |
| collection | DOAJ |
| description | The larvae of Drosophila melanogaster is a model organism widely used to study the muscular and nervous systems. Drosophila larvae are surrounded by a waxy cuticle that prevents permeation by most substances. Here we develop a method to remove this layer, rendering the larvae permeable to small molecules without causing death, allowing the larvae to develop to adulthood and reproduce. Permeability was assessed using fluorescein diacetate dye uptake, and mortality upon exposure to toxic levels of ethylene glycol (EG) and Dimethyl sulfoxide (DMSO). Potential uses for this method include drug delivery, toxicity assays, cryopreservation, staining, and fixation. |
| first_indexed | 2024-03-11T22:59:07Z |
| format | Article |
| id | doaj.art-fefc2072f9ab43e88d82bec949003ed1 |
| institution | Directory Open Access Journal |
| issn | 1933-6934 1933-6942 |
| language | English |
| last_indexed | 2024-03-11T22:59:07Z |
| publishDate | 2020-10-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Fly |
| spelling | doaj.art-fefc2072f9ab43e88d82bec949003ed12023-09-21T15:09:08ZengTaylor & Francis GroupFly1933-69341933-69422020-10-01141-4293310.1080/19336934.2020.17240511724051A method for the permeabilization of live Drosophila melanogaster larvae to small molecules and cryoprotectantsAlex Murray0Daniel Palmer1Daimark Bennett2Venkata Dwarampudi3João Pedro de Magalhães4University of LiverpoolUniversity of LiverpoolUniversity of LiverpoolLancaster UniversityUniversity of LiverpoolThe larvae of Drosophila melanogaster is a model organism widely used to study the muscular and nervous systems. Drosophila larvae are surrounded by a waxy cuticle that prevents permeation by most substances. Here we develop a method to remove this layer, rendering the larvae permeable to small molecules without causing death, allowing the larvae to develop to adulthood and reproduce. Permeability was assessed using fluorescein diacetate dye uptake, and mortality upon exposure to toxic levels of ethylene glycol (EG) and Dimethyl sulfoxide (DMSO). Potential uses for this method include drug delivery, toxicity assays, cryopreservation, staining, and fixation.http://dx.doi.org/10.1080/19336934.2020.1724051drosophilalarvaepermeabilizationimagingcryobiologycryopreservationmethod |
| spellingShingle | Alex Murray Daniel Palmer Daimark Bennett Venkata Dwarampudi João Pedro de Magalhães A method for the permeabilization of live Drosophila melanogaster larvae to small molecules and cryoprotectants Fly drosophila larvae permeabilization imaging cryobiology cryopreservation method |
| title | A method for the permeabilization of live Drosophila melanogaster larvae to small molecules and cryoprotectants |
| title_full | A method for the permeabilization of live Drosophila melanogaster larvae to small molecules and cryoprotectants |
| title_fullStr | A method for the permeabilization of live Drosophila melanogaster larvae to small molecules and cryoprotectants |
| title_full_unstemmed | A method for the permeabilization of live Drosophila melanogaster larvae to small molecules and cryoprotectants |
| title_short | A method for the permeabilization of live Drosophila melanogaster larvae to small molecules and cryoprotectants |
| title_sort | method for the permeabilization of live drosophila melanogaster larvae to small molecules and cryoprotectants |
| topic | drosophila larvae permeabilization imaging cryobiology cryopreservation method |
| url | http://dx.doi.org/10.1080/19336934.2020.1724051 |
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