Sm14 gene expression in different stages of the Schistosoma mansoni life cycle and immunolocalization of the Sm14 protein within the adult worm
Sm14 is a 14-kDa vaccine candidate antigen from Schistosoma mansoni that seems to be involved in cytoplasmic trafficking of fatty acids. Although schistosomes have a high requirement for lipids, they are not able to synthesize fatty acids and sterols de novo. Thus, they must acquire host lipids. In...
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| Format: | Article |
| Language: | English |
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Associação Brasileira de Divulgação Científica
2002-03-01
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| Series: | Brazilian Journal of Medical and Biological Research |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002000300014 |
| _version_ | 1811308541661675520 |
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| author | C.F.A. Brito G.C. Oliveira S.C. Oliveira M. Street S. Riengrojpitak R.A. Wilson A.J.G. Simpson R. Correa-Oliveira |
| author_facet | C.F.A. Brito G.C. Oliveira S.C. Oliveira M. Street S. Riengrojpitak R.A. Wilson A.J.G. Simpson R. Correa-Oliveira |
| author_sort | C.F.A. Brito |
| collection | DOAJ |
| description | Sm14 is a 14-kDa vaccine candidate antigen from Schistosoma mansoni that seems to be involved in cytoplasmic trafficking of fatty acids. Although schistosomes have a high requirement for lipids, they are not able to synthesize fatty acids and sterols de novo. Thus, they must acquire host lipids. In order to determine whether Sm14 is present in different stages of the life cycle of the parasite, we performed RT-PCR. Sm14 mRNA was identified in all stages of the life cycle studied, mainly schistosomulum, adult worm and egg. Additionally, we used a rabbit anti-Sm14 polyclonal antibody in an indirect immunofluorescence assay to localize Sm14 in adult worm sections. The basal lamella of the tegument and the gut epithelium were strongly labeled. These tissues have a high flow of and demand for lipids, a finding that supports the putative role of Sm14 as an intracellular transporter of fatty acids from host cells. |
| first_indexed | 2024-04-13T09:25:02Z |
| format | Article |
| id | doaj.art-ff0015e3619e4965b4b2a48b57a0fdb4 |
| institution | Directory Open Access Journal |
| issn | 0100-879X 1414-431X |
| language | English |
| last_indexed | 2024-04-13T09:25:02Z |
| publishDate | 2002-03-01 |
| publisher | Associação Brasileira de Divulgação Científica |
| record_format | Article |
| series | Brazilian Journal of Medical and Biological Research |
| spelling | doaj.art-ff0015e3619e4965b4b2a48b57a0fdb42022-12-22T02:52:30ZengAssociação Brasileira de Divulgação CientíficaBrazilian Journal of Medical and Biological Research0100-879X1414-431X2002-03-0135337738110.1590/S0100-879X2002000300014Sm14 gene expression in different stages of the Schistosoma mansoni life cycle and immunolocalization of the Sm14 protein within the adult wormC.F.A. BritoG.C. OliveiraS.C. OliveiraM. StreetS. RiengrojpitakR.A. WilsonA.J.G. SimpsonR. Correa-OliveiraSm14 is a 14-kDa vaccine candidate antigen from Schistosoma mansoni that seems to be involved in cytoplasmic trafficking of fatty acids. Although schistosomes have a high requirement for lipids, they are not able to synthesize fatty acids and sterols de novo. Thus, they must acquire host lipids. In order to determine whether Sm14 is present in different stages of the life cycle of the parasite, we performed RT-PCR. Sm14 mRNA was identified in all stages of the life cycle studied, mainly schistosomulum, adult worm and egg. Additionally, we used a rabbit anti-Sm14 polyclonal antibody in an indirect immunofluorescence assay to localize Sm14 in adult worm sections. The basal lamella of the tegument and the gut epithelium were strongly labeled. These tissues have a high flow of and demand for lipids, a finding that supports the putative role of Sm14 as an intracellular transporter of fatty acids from host cells.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002000300014Sm14Fatty acid-binding proteinFABPImmunolocalizationRT-PCR |
| spellingShingle | C.F.A. Brito G.C. Oliveira S.C. Oliveira M. Street S. Riengrojpitak R.A. Wilson A.J.G. Simpson R. Correa-Oliveira Sm14 gene expression in different stages of the Schistosoma mansoni life cycle and immunolocalization of the Sm14 protein within the adult worm Brazilian Journal of Medical and Biological Research Sm14 Fatty acid-binding protein FABP Immunolocalization RT-PCR |
| title | Sm14 gene expression in different stages of the Schistosoma mansoni life cycle and immunolocalization of the Sm14 protein within the adult worm |
| title_full | Sm14 gene expression in different stages of the Schistosoma mansoni life cycle and immunolocalization of the Sm14 protein within the adult worm |
| title_fullStr | Sm14 gene expression in different stages of the Schistosoma mansoni life cycle and immunolocalization of the Sm14 protein within the adult worm |
| title_full_unstemmed | Sm14 gene expression in different stages of the Schistosoma mansoni life cycle and immunolocalization of the Sm14 protein within the adult worm |
| title_short | Sm14 gene expression in different stages of the Schistosoma mansoni life cycle and immunolocalization of the Sm14 protein within the adult worm |
| title_sort | sm14 gene expression in different stages of the schistosoma mansoni life cycle and immunolocalization of the sm14 protein within the adult worm |
| topic | Sm14 Fatty acid-binding protein FABP Immunolocalization RT-PCR |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002000300014 |
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