A Lectin Disrupts Vector Transmission of a Grapevine Ampelovirus
Grapevine leafroll disease is one of the most important virus diseases of grapevines and occurs in every major grape-growing region of the world. The vector-transmission mechanisms of the causative agent, <i>Grapevine leafroll-associated virus 3</i> (GLRaV-3), remain poorly understood. W...
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| Format: | Article |
| Language: | English |
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MDPI AG
2020-08-01
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| Series: | Viruses |
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| Online Access: | https://www.mdpi.com/1999-4915/12/8/843 |
| _version_ | 1797560580747296768 |
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| author | Cecilia A. Prator Rodrigo P. P. Almeida |
| author_facet | Cecilia A. Prator Rodrigo P. P. Almeida |
| author_sort | Cecilia A. Prator |
| collection | DOAJ |
| description | Grapevine leafroll disease is one of the most important virus diseases of grapevines and occurs in every major grape-growing region of the world. The vector-transmission mechanisms of the causative agent, <i>Grapevine leafroll-associated virus 3</i> (GLRaV-3), remain poorly understood. We show that the vine mealybug, <i>Planococcus ficus</i>, feeds through a membrane feeding system on GLRaV-3 viral purifications from both <i>V. vinifera</i> and <i>N. benthamiana</i> and transmits the virus to test plants from plants from both species. Building on this strategy, we used an immunofluorescence approach to localize virions to two retention sites in <i>P. ficus</i> mouthparts. Assays testing molecules capable of blocking virus transmission demonstrated that GLRaV-3-transmission by <i>P. ficus</i> could be disrupted. Our results indicate that our membrane feeding system and transmission-blocking assays are a valid approach and can be used to screen other candidate blocking molecules. |
| first_indexed | 2024-03-10T18:02:46Z |
| format | Article |
| id | doaj.art-ff0588da2a3c456c99ed0e59460a69a1 |
| institution | Directory Open Access Journal |
| issn | 1999-4915 |
| language | English |
| last_indexed | 2024-03-10T18:02:46Z |
| publishDate | 2020-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Viruses |
| spelling | doaj.art-ff0588da2a3c456c99ed0e59460a69a12023-11-20T08:49:14ZengMDPI AGViruses1999-49152020-08-0112884310.3390/v12080843A Lectin Disrupts Vector Transmission of a Grapevine AmpelovirusCecilia A. Prator0Rodrigo P. P. Almeida1Department of Environmental Science, Policy and Management, University of California, Berkeley, CA 94720, USADepartment of Environmental Science, Policy and Management, University of California, Berkeley, CA 94720, USAGrapevine leafroll disease is one of the most important virus diseases of grapevines and occurs in every major grape-growing region of the world. The vector-transmission mechanisms of the causative agent, <i>Grapevine leafroll-associated virus 3</i> (GLRaV-3), remain poorly understood. We show that the vine mealybug, <i>Planococcus ficus</i>, feeds through a membrane feeding system on GLRaV-3 viral purifications from both <i>V. vinifera</i> and <i>N. benthamiana</i> and transmits the virus to test plants from plants from both species. Building on this strategy, we used an immunofluorescence approach to localize virions to two retention sites in <i>P. ficus</i> mouthparts. Assays testing molecules capable of blocking virus transmission demonstrated that GLRaV-3-transmission by <i>P. ficus</i> could be disrupted. Our results indicate that our membrane feeding system and transmission-blocking assays are a valid approach and can be used to screen other candidate blocking molecules.https://www.mdpi.com/1999-4915/12/8/843Grapevine leafroll-associated virus 3Grapevine leafroll diseaseClosteroviridae<i>Ampelovirus</i><i>Planococcus ficus</i>vine mealybug |
| spellingShingle | Cecilia A. Prator Rodrigo P. P. Almeida A Lectin Disrupts Vector Transmission of a Grapevine Ampelovirus Viruses Grapevine leafroll-associated virus 3 Grapevine leafroll disease Closteroviridae <i>Ampelovirus</i> <i>Planococcus ficus</i> vine mealybug |
| title | A Lectin Disrupts Vector Transmission of a Grapevine Ampelovirus |
| title_full | A Lectin Disrupts Vector Transmission of a Grapevine Ampelovirus |
| title_fullStr | A Lectin Disrupts Vector Transmission of a Grapevine Ampelovirus |
| title_full_unstemmed | A Lectin Disrupts Vector Transmission of a Grapevine Ampelovirus |
| title_short | A Lectin Disrupts Vector Transmission of a Grapevine Ampelovirus |
| title_sort | lectin disrupts vector transmission of a grapevine ampelovirus |
| topic | Grapevine leafroll-associated virus 3 Grapevine leafroll disease Closteroviridae <i>Ampelovirus</i> <i>Planococcus ficus</i> vine mealybug |
| url | https://www.mdpi.com/1999-4915/12/8/843 |
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