Low Sperm Motility Is Determined by Abnormal Protein Modification during Epididymal Maturation
Purpose:Purpose: During epididymal sperm maturation, spermatozoa acquire progressive motility through dynamic protein modifica-tions. However, the relationship between sequential protein modifications during epididymal sperm maturation and sperm motility and fertility has not yet been investigated....
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| Format: | Article |
| Language: | English |
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Korean Society for Sexual Medicine and Andrology
2022-07-01
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| Series: | The World Journal of Men's Health |
| Subjects: |
| _version_ | 1818214885489639424 |
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| author | Yoo-Jin Park Byeong-Mu Lee Won-Ki Pang Do-Yeal Ryu Md Saidur Rahman Myung-Geol Pang |
| author_facet | Yoo-Jin Park Byeong-Mu Lee Won-Ki Pang Do-Yeal Ryu Md Saidur Rahman Myung-Geol Pang |
| author_sort | Yoo-Jin Park |
| collection | DOAJ |
| description | Purpose:Purpose: During epididymal sperm maturation, spermatozoa acquire progressive motility through dynamic protein modifica-tions. However, the relationship between sequential protein modifications during epididymal sperm maturation and sperm motility and fertility has not yet been investigated. This study investigated whether sequential changes in fertility-related protein expression including that of enolase 1 (ENO1), ubiquinol-cytochrome c reductase core protein 1 and 2 (UQCRC1 and UQCRC2), and voltage-dependent anion channel 2 (VDAC2) in spermatozoa during epididymal maturation are related to bovine sperm motility. Moreover, we found that mitochondrial metabolism is closely related to fertility-related proteins. Therefore, we investigated how the sequential modification of mitochondrial proteins during epididymal maturation regulates sperm motility.
Materials and Methods:Materials and Methods: To determine the differential protein expression in caput and cauda epididymal spermatozoa from low and high motility bulls, western blot analysis was performed. Moreover, signaling pathways were identified to under-stand the mechanisms of regulation of sperm motility through the differential protein expression associated with fertility-related proteins.
Results:Results: We found that ENO1 was substantially higher in the caput spermatozoa from low motility bulls than the caput and cauda spermatozoa from high motility bulls. However, ENO1 expression in low motility bull spermatozoa was downregu-lated to a level comparable to that in the high motility bull spermatozoa during epididymal maturation. Moreover, there was a lack of modification of mitochondrial proteins, including glutathione peroxidase 4 and NADH:Ubiquinone Oxidoreductase Core Subunit S8, in low motility bull spermatozoa during epididymal maturation, whereas active changes were detected in high motility bull spermatozoa.
Conclusions:Conclusions: Irregular modifications of mitochondrial proteins during epididymal sperm maturation may increase excessive ROS production and premature activation of spermatozoa during epididymal maturation. Consequently, spermatozoa may lose their motility by the earlier consumption of their energy source and may be damaged by ROS during epididymal matura-tion, resulting in a decline in sperm motility and bull fertility. |
| first_indexed | 2024-12-12T06:27:17Z |
| format | Article |
| id | doaj.art-ff058add8fd44240959c6d859ed0bd30 |
| institution | Directory Open Access Journal |
| issn | 2287-4208 2287-4690 |
| language | English |
| last_indexed | 2024-12-12T06:27:17Z |
| publishDate | 2022-07-01 |
| publisher | Korean Society for Sexual Medicine and Andrology |
| record_format | Article |
| series | The World Journal of Men's Health |
| spelling | doaj.art-ff058add8fd44240959c6d859ed0bd302022-12-22T00:34:44ZengKorean Society for Sexual Medicine and AndrologyThe World Journal of Men's Health2287-42082287-46902022-07-0140352653510.5534/wjmh.210180Low Sperm Motility Is Determined by Abnormal Protein Modification during Epididymal MaturationYoo-Jin Park0https://orcid.org/0000-0002-0733-800XByeong-Mu Lee1https://orcid.org/0000-0003-4344-9572Won-Ki Pang2https://orcid.org/0000-0002-8600-5499Do-Yeal Ryu3https://orcid.org/0000-0003-2927-3267Md Saidur Rahman4https://orcid.org/0000-0003-4583-8731Myung-Geol Pang5https://orcid.org/0000-0003-2019-3789Department of Animal Science & Technology and BET Research Institute, Chung-Ang University, Anseong, KoreaDepartment of Animal Science & Technology and BET Research Institute, Chung-Ang University, Anseong, KoreaDepartment of Animal Science & Technology and BET Research Institute, Chung-Ang University, Anseong, KoreaDepartment of Animal Science & Technology and BET Research Institute, Chung-Ang University, Anseong, KoreaDepartment of Animal Science & Technology and BET Research Institute, Chung-Ang University, Anseong, KoreaDepartment of Animal Science & Technology and BET Research Institute, Chung-Ang University, Anseong, KoreaPurpose:Purpose: During epididymal sperm maturation, spermatozoa acquire progressive motility through dynamic protein modifica-tions. However, the relationship between sequential protein modifications during epididymal sperm maturation and sperm motility and fertility has not yet been investigated. This study investigated whether sequential changes in fertility-related protein expression including that of enolase 1 (ENO1), ubiquinol-cytochrome c reductase core protein 1 and 2 (UQCRC1 and UQCRC2), and voltage-dependent anion channel 2 (VDAC2) in spermatozoa during epididymal maturation are related to bovine sperm motility. Moreover, we found that mitochondrial metabolism is closely related to fertility-related proteins. Therefore, we investigated how the sequential modification of mitochondrial proteins during epididymal maturation regulates sperm motility. Materials and Methods:Materials and Methods: To determine the differential protein expression in caput and cauda epididymal spermatozoa from low and high motility bulls, western blot analysis was performed. Moreover, signaling pathways were identified to under-stand the mechanisms of regulation of sperm motility through the differential protein expression associated with fertility-related proteins. Results:Results: We found that ENO1 was substantially higher in the caput spermatozoa from low motility bulls than the caput and cauda spermatozoa from high motility bulls. However, ENO1 expression in low motility bull spermatozoa was downregu-lated to a level comparable to that in the high motility bull spermatozoa during epididymal maturation. Moreover, there was a lack of modification of mitochondrial proteins, including glutathione peroxidase 4 and NADH:Ubiquinone Oxidoreductase Core Subunit S8, in low motility bull spermatozoa during epididymal maturation, whereas active changes were detected in high motility bull spermatozoa. Conclusions:Conclusions: Irregular modifications of mitochondrial proteins during epididymal sperm maturation may increase excessive ROS production and premature activation of spermatozoa during epididymal maturation. Consequently, spermatozoa may lose their motility by the earlier consumption of their energy source and may be damaged by ROS during epididymal matura-tion, resulting in a decline in sperm motility and bull fertility.epididymisfertilityproteomesperm maturationsperm motility |
| spellingShingle | Yoo-Jin Park Byeong-Mu Lee Won-Ki Pang Do-Yeal Ryu Md Saidur Rahman Myung-Geol Pang Low Sperm Motility Is Determined by Abnormal Protein Modification during Epididymal Maturation The World Journal of Men's Health epididymis fertility proteome sperm maturation sperm motility |
| title | Low Sperm Motility Is Determined by Abnormal Protein Modification during Epididymal Maturation |
| title_full | Low Sperm Motility Is Determined by Abnormal Protein Modification during Epididymal Maturation |
| title_fullStr | Low Sperm Motility Is Determined by Abnormal Protein Modification during Epididymal Maturation |
| title_full_unstemmed | Low Sperm Motility Is Determined by Abnormal Protein Modification during Epididymal Maturation |
| title_short | Low Sperm Motility Is Determined by Abnormal Protein Modification during Epididymal Maturation |
| title_sort | low sperm motility is determined by abnormal protein modification during epididymal maturation |
| topic | epididymis fertility proteome sperm maturation sperm motility |
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