A NEW MODEL OF BOAR SEMEN EVALUATION AND THE IMPACT OF CRYOGENIC FACTOR ON SPERMATIC CELLS
Nowadays, sperm evaluation is mostly used to predict fertility and freezability. The aim of this study is to evaluate the possibility of investigating the effects of the cryogenic agent on boar spermatozoa, by identifying a set of laboratory tests for a rapid and efficient evaluation of semen qualit...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
| Published: |
Agroprint Timisoara
2023-10-01
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| Series: | Scientific Papers Animal Science and Biotechnologies |
| Subjects: | |
| Online Access: | https://spasb.ro/index.php/public_html/article/view/1186 |
| Summary: | Nowadays, sperm evaluation is mostly used to predict fertility and freezability. The
aim of this study is to evaluate the possibility of investigating the effects of the
cryogenic agent on boar spermatozoa, by identifying a set of laboratory tests for a
rapid and efficient evaluation of semen quality. Usual sperm analysis such as sperm
concentration, motility and spermatozoa morphology are not able to show subtle
abnormalities, which are having a basic role in the fertilizing ability. Moreover, it
seems that other sperm characteristics, involved in the fertilizing ability, can interfere
with the freezing-thawing processes, being not evaluated or maybe not known.
Morphological (microscopic analysis of stained spermatozoa), functional (motility
analysis and hypo-osmotic swelling test) and chromatin integrity (Acridine Orange
Test and Comet Assay) analysis were performed aiming to show the differences in
spermatozoon integrity and functionality, caused by the cryogenic factor. |
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| ISSN: | 1841-9364 2344-4576 |