In Vitro Induction of Interspecific Hybrid and Polyploidy Derived from <i>Oryza officinalis</i> Wall
<i>Oryza officinalis</i> Wall is a potential genetic resource for rice breeding; however, its distant genome limits its crossing ability with cultivated rice. The interspecific hybridization of <i>O. officinalis</i> and cultivated rice, establishment of its tissue culture, an...
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MDPI AG
2023-08-01
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| Series: | Plants |
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| Online Access: | https://www.mdpi.com/2223-7747/12/16/3001 |
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| author | Meimei Tan Ruoxin Chen Xingran Chen Muhammad Qasim Shahid Xiangdong Liu Jinwen Wu |
| author_facet | Meimei Tan Ruoxin Chen Xingran Chen Muhammad Qasim Shahid Xiangdong Liu Jinwen Wu |
| author_sort | Meimei Tan |
| collection | DOAJ |
| description | <i>Oryza officinalis</i> Wall is a potential genetic resource for rice breeding; however, its distant genome limits its crossing ability with cultivated rice. The interspecific hybridization of <i>O. officinalis</i> and cultivated rice, establishment of its tissue culture, and induction of polyploidy are ways to improve <i>O. officinalis</i>’s poor crossability. We developed an interspecific hybrid and studied its reproductive pollen development process in this work, and the results showed that abortive pollens (81.94%) and embryo sac abnormalities (91.04%) were the key causes of its high sterility. In order to induce callus formation in interspecific hybrid explants, two different culture media, namely Chu’s N-6 medium (N6) and 1/2 Murashig and Skoog medium (1/2 MS), were employed. Additionally, two plant growth regulators (PGRs), namely 2,4-dichlorophenoxyacetic acid (2,4-D) and 6-benzylaminopurine (BA), along with L-proline (Pro) and acid hydrolyzed casein, were utilized in the experiment. The optimal N6 medium, supplemented with 2.0 mg·L<sup>−1</sup> 2,4-D, produced the highest induction rate (80.56 ± 5.44)%. For callus differentiation and proliferation, the MS medium supplemented with 2.0 mg·L<sup>−1</sup> BA + 0.2 mg·L<sup>−1</sup> NAA produced the highest differentiation rate (75.00 ± 4.97)% and seedling emergence ratio (28.97 ± 4.67)%. The optimal combination for seedling rooting was the 1/2 MS medium supplemented with 2.0 mg L<sup>−1</sup> NAA and 0.2 mg L<sup>−1</sup> BA, which produced an average of 13.95 roots per plant. For polyploidy induction in the interspecific hybrid, the concentration of colchicine treatment at 400 mg·L<sup>−1</sup> for three days is an ideal protocol. We devised tissue culture and interspecific hybrid polyploidy induction to overcome <i>O. officinalis</i>’ poor crossability and introduce its favorable features into cultivated rice. |
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| institution | Directory Open Access Journal |
| issn | 2223-7747 |
| language | English |
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| spelling | doaj.art-ff08ff52b9914e8fb499b5c8c70633fc2023-11-19T02:42:14ZengMDPI AGPlants2223-77472023-08-011216300110.3390/plants12163001In Vitro Induction of Interspecific Hybrid and Polyploidy Derived from <i>Oryza officinalis</i> WallMeimei Tan0Ruoxin Chen1Xingran Chen2Muhammad Qasim Shahid3Xiangdong Liu4Jinwen Wu5State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, South China Agricultural University, Guangzhou 510642, ChinaState Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, South China Agricultural University, Guangzhou 510642, ChinaState Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, South China Agricultural University, Guangzhou 510642, ChinaState Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, South China Agricultural University, Guangzhou 510642, ChinaState Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, South China Agricultural University, Guangzhou 510642, ChinaState Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, South China Agricultural University, Guangzhou 510642, China<i>Oryza officinalis</i> Wall is a potential genetic resource for rice breeding; however, its distant genome limits its crossing ability with cultivated rice. The interspecific hybridization of <i>O. officinalis</i> and cultivated rice, establishment of its tissue culture, and induction of polyploidy are ways to improve <i>O. officinalis</i>’s poor crossability. We developed an interspecific hybrid and studied its reproductive pollen development process in this work, and the results showed that abortive pollens (81.94%) and embryo sac abnormalities (91.04%) were the key causes of its high sterility. In order to induce callus formation in interspecific hybrid explants, two different culture media, namely Chu’s N-6 medium (N6) and 1/2 Murashig and Skoog medium (1/2 MS), were employed. Additionally, two plant growth regulators (PGRs), namely 2,4-dichlorophenoxyacetic acid (2,4-D) and 6-benzylaminopurine (BA), along with L-proline (Pro) and acid hydrolyzed casein, were utilized in the experiment. The optimal N6 medium, supplemented with 2.0 mg·L<sup>−1</sup> 2,4-D, produced the highest induction rate (80.56 ± 5.44)%. For callus differentiation and proliferation, the MS medium supplemented with 2.0 mg·L<sup>−1</sup> BA + 0.2 mg·L<sup>−1</sup> NAA produced the highest differentiation rate (75.00 ± 4.97)% and seedling emergence ratio (28.97 ± 4.67)%. The optimal combination for seedling rooting was the 1/2 MS medium supplemented with 2.0 mg L<sup>−1</sup> NAA and 0.2 mg L<sup>−1</sup> BA, which produced an average of 13.95 roots per plant. For polyploidy induction in the interspecific hybrid, the concentration of colchicine treatment at 400 mg·L<sup>−1</sup> for three days is an ideal protocol. We devised tissue culture and interspecific hybrid polyploidy induction to overcome <i>O. officinalis</i>’ poor crossability and introduce its favorable features into cultivated rice.https://www.mdpi.com/2223-7747/12/16/3001rice<i>Oryza officinalis</i> Walltissue culturepolyploidy |
| spellingShingle | Meimei Tan Ruoxin Chen Xingran Chen Muhammad Qasim Shahid Xiangdong Liu Jinwen Wu In Vitro Induction of Interspecific Hybrid and Polyploidy Derived from <i>Oryza officinalis</i> Wall Plants rice <i>Oryza officinalis</i> Wall tissue culture polyploidy |
| title | In Vitro Induction of Interspecific Hybrid and Polyploidy Derived from <i>Oryza officinalis</i> Wall |
| title_full | In Vitro Induction of Interspecific Hybrid and Polyploidy Derived from <i>Oryza officinalis</i> Wall |
| title_fullStr | In Vitro Induction of Interspecific Hybrid and Polyploidy Derived from <i>Oryza officinalis</i> Wall |
| title_full_unstemmed | In Vitro Induction of Interspecific Hybrid and Polyploidy Derived from <i>Oryza officinalis</i> Wall |
| title_short | In Vitro Induction of Interspecific Hybrid and Polyploidy Derived from <i>Oryza officinalis</i> Wall |
| title_sort | in vitro induction of interspecific hybrid and polyploidy derived from i oryza officinalis i wall |
| topic | rice <i>Oryza officinalis</i> Wall tissue culture polyploidy |
| url | https://www.mdpi.com/2223-7747/12/16/3001 |
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