Vibrational Spectroscopy for In Vitro Monitoring Stem Cell Differentiation
Stem cell technology has attracted considerable attention over recent decades due to its enormous potential in regenerative medicine and disease therapeutics. Studying the underlying mechanisms of stem cell differentiation and tissue generation is critical, and robust methodologies and different tec...
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| Format: | Article |
| Language: | English |
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MDPI AG
2020-11-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/25/23/5554 |
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| author | Francesca Ravera Esen Efeoglu Hugh J. Byrne |
| author_facet | Francesca Ravera Esen Efeoglu Hugh J. Byrne |
| author_sort | Francesca Ravera |
| collection | DOAJ |
| description | Stem cell technology has attracted considerable attention over recent decades due to its enormous potential in regenerative medicine and disease therapeutics. Studying the underlying mechanisms of stem cell differentiation and tissue generation is critical, and robust methodologies and different technologies are required. Towards establishing improved understanding and optimised triggering and control of differentiation processes, analytical techniques such as flow cytometry, immunohistochemistry, reverse transcription polymerase chain reaction, RNA in situ hybridisation analysis, and fluorescence-activated cell sorting have contributed much. However, progress in the field remains limited because such techniques provide only limited information, as they are only able to address specific, selected aspects of the process, and/or cannot visualise the process at the subcellular level. Additionally, many current analytical techniques involve the disruption of the investigation process (tissue sectioning, immunostaining) and cannot monitor the cellular differentiation process in situ, in real-time. Vibrational spectroscopy, as a label-free, non-invasive and non-destructive analytical technique, appears to be a promising candidate to potentially overcome many of these limitations as it can provide detailed biochemical fingerprint information for analysis of cells, tissues, and body fluids. The technique has been widely used in disease diagnosis and increasingly in stem cell technology. In this work, the efforts regarding the use of vibrational spectroscopy to identify mechanisms of stem cell differentiation at a single cell and tissue level are summarised. Both infrared absorption and Raman spectroscopic investigations are explored, and the relative merits, and future perspectives of the techniques are discussed. |
| first_indexed | 2024-03-10T14:32:37Z |
| format | Article |
| id | doaj.art-ff0a415209184bd7b2bab3ab55e6f574 |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-10T14:32:37Z |
| publishDate | 2020-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-ff0a415209184bd7b2bab3ab55e6f5742023-11-20T22:28:37ZengMDPI AGMolecules1420-30492020-11-012523555410.3390/molecules25235554Vibrational Spectroscopy for In Vitro Monitoring Stem Cell DifferentiationFrancesca Ravera0Esen Efeoglu1Hugh J. Byrne2School of Physics and Clinical and Optometric Sciences, Technological University Dublin, City Campus, 8 Dublin, IrelandSchool of Biomolecular and Biomedical Science, University College Dublin, Belfield, 4 Dublin, IrelandFOCAS Research Institute, Technological University Dublin, City Campus, 8 Dublin, IrelandStem cell technology has attracted considerable attention over recent decades due to its enormous potential in regenerative medicine and disease therapeutics. Studying the underlying mechanisms of stem cell differentiation and tissue generation is critical, and robust methodologies and different technologies are required. Towards establishing improved understanding and optimised triggering and control of differentiation processes, analytical techniques such as flow cytometry, immunohistochemistry, reverse transcription polymerase chain reaction, RNA in situ hybridisation analysis, and fluorescence-activated cell sorting have contributed much. However, progress in the field remains limited because such techniques provide only limited information, as they are only able to address specific, selected aspects of the process, and/or cannot visualise the process at the subcellular level. Additionally, many current analytical techniques involve the disruption of the investigation process (tissue sectioning, immunostaining) and cannot monitor the cellular differentiation process in situ, in real-time. Vibrational spectroscopy, as a label-free, non-invasive and non-destructive analytical technique, appears to be a promising candidate to potentially overcome many of these limitations as it can provide detailed biochemical fingerprint information for analysis of cells, tissues, and body fluids. The technique has been widely used in disease diagnosis and increasingly in stem cell technology. In this work, the efforts regarding the use of vibrational spectroscopy to identify mechanisms of stem cell differentiation at a single cell and tissue level are summarised. Both infrared absorption and Raman spectroscopic investigations are explored, and the relative merits, and future perspectives of the techniques are discussed.https://www.mdpi.com/1420-3049/25/23/5554stem cell technologyvibrational spectroscopyRaman spectroscopyFTIR absorption spectroscopystem cell differentiationspectral markers |
| spellingShingle | Francesca Ravera Esen Efeoglu Hugh J. Byrne Vibrational Spectroscopy for In Vitro Monitoring Stem Cell Differentiation Molecules stem cell technology vibrational spectroscopy Raman spectroscopy FTIR absorption spectroscopy stem cell differentiation spectral markers |
| title | Vibrational Spectroscopy for In Vitro Monitoring Stem Cell Differentiation |
| title_full | Vibrational Spectroscopy for In Vitro Monitoring Stem Cell Differentiation |
| title_fullStr | Vibrational Spectroscopy for In Vitro Monitoring Stem Cell Differentiation |
| title_full_unstemmed | Vibrational Spectroscopy for In Vitro Monitoring Stem Cell Differentiation |
| title_short | Vibrational Spectroscopy for In Vitro Monitoring Stem Cell Differentiation |
| title_sort | vibrational spectroscopy for in vitro monitoring stem cell differentiation |
| topic | stem cell technology vibrational spectroscopy Raman spectroscopy FTIR absorption spectroscopy stem cell differentiation spectral markers |
| url | https://www.mdpi.com/1420-3049/25/23/5554 |
| work_keys_str_mv | AT francescaravera vibrationalspectroscopyforinvitromonitoringstemcelldifferentiation AT esenefeoglu vibrationalspectroscopyforinvitromonitoringstemcelldifferentiation AT hughjbyrne vibrationalspectroscopyforinvitromonitoringstemcelldifferentiation |