Biocontrol Potential of Some Rhizospheric Soil Bacterial Strains against <i>Fusarium culmorum</i> and Subsequent Effect on Growth of Two Tunisian Wheat Cultivars
PGPR (Plant Growth Promoting Rhizobacteria) are used as biofertilizers and biological control agents against fungi. The objective of this work was to evaluate the antagonistic activities of some bacterial strains isolated from soil against four phytopathogenic fungal strains (<i>Fusarium grami...
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MDPI AG
2023-04-01
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| Series: | Microorganisms |
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| Online Access: | https://www.mdpi.com/2076-2607/11/5/1165 |
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| author | Habiba Kouki Mouna Souihi Ilhem Saadouli Sabrine Balti Amira Ayed Nihed Majdoub Amor Mosbah Ismail Amri Yassine Mabrouk |
| author_facet | Habiba Kouki Mouna Souihi Ilhem Saadouli Sabrine Balti Amira Ayed Nihed Majdoub Amor Mosbah Ismail Amri Yassine Mabrouk |
| author_sort | Habiba Kouki |
| collection | DOAJ |
| description | PGPR (Plant Growth Promoting Rhizobacteria) are used as biofertilizers and biological control agents against fungi. The objective of this work was to evaluate the antagonistic activities of some bacterial strains isolated from soil against four phytopathogenic fungal strains (<i>Fusarium graminearum</i>, <i>F. culmorum</i>, <i>Phytophthora</i> sp. and <i>Verticillium dahlia</i>). Two strains having an antagonist effect on fungi and displaying the maximum of plant growth promoting (PGP) traits were selected for further study and identified as <i>Bacillus subtilis</i> and <i>B. amyloliquefaciens</i> respectively. <i>In planta</i> assays demonstrated that the two <i>Bacillus</i> strains are able to enhance plant growth of two wheat cultivars in absence of nitrogen and protect them against <i>F. culmorum</i>. Pot experiments performed in a greenhouse showed that wheat plants inoculation with two bacterial strains reduce <i>F. culmorum</i> disease severity correlated with the accumulation of phenolic compounds and chlorophyll content. These could partly explain the effectiveness of these bacteria in protecting Tunisian durum wheat cultivars against <i>F. culmorum</i>. Application <i>B. amyloliquefaciens</i>, showed better protection than <i>B. subtilis</i> although the last one enhanced more the plant growth of two wheat cultivars in absence of fungus. Hence, combination of two bacterial strains could be a strategic approach to enhance plant growth and control plant diseases. |
| first_indexed | 2024-03-11T03:28:57Z |
| format | Article |
| id | doaj.art-ff0be4b5aa5f49c3a99e7d70ad4a8ec5 |
| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-03-11T03:28:57Z |
| publishDate | 2023-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microorganisms |
| spelling | doaj.art-ff0be4b5aa5f49c3a99e7d70ad4a8ec52023-11-18T02:32:46ZengMDPI AGMicroorganisms2076-26072023-04-01115116510.3390/microorganisms11051165Biocontrol Potential of Some Rhizospheric Soil Bacterial Strains against <i>Fusarium culmorum</i> and Subsequent Effect on Growth of Two Tunisian Wheat CultivarsHabiba Kouki0Mouna Souihi1Ilhem Saadouli2Sabrine Balti3Amira Ayed4Nihed Majdoub5Amor Mosbah6Ismail Amri7Yassine Mabrouk8Laboratory of Biotechnology and Nuclear Technology, National Centre for Nuclear Sciences and Technologies (CNSTN), Sidi Thabet, Technopark, Ariana 2020, TunisiaLaboratory of Biotechnology and Nuclear Technology, National Centre for Nuclear Sciences and Technologies (CNSTN), Sidi Thabet, Technopark, Ariana 2020, TunisiaLaboratory of Microorganisms and Active Biomolecules, Faculty of Sciences of Tunis, University of Tunis El Manar, Tunis 2092, TunisiaLaboratory of Biotechnology and Nuclear Technology, National Centre for Nuclear Sciences and Technologies (CNSTN), Sidi Thabet, Technopark, Ariana 2020, TunisiaLaboratory of Biotechnology and Nuclear Technology, National Centre for Nuclear Sciences and Technologies (CNSTN), Sidi Thabet, Technopark, Ariana 2020, TunisiaLaboratory of Biotechnology and Nuclear Technology, National Centre for Nuclear Sciences and Technologies (CNSTN), Sidi Thabet, Technopark, Ariana 2020, TunisiaLaboratory of Biotechnology and Valorization of Bio-Geo Resources, Higher Institute of Biotechnology of Sidi Thabet (ISBST), University of Manouba, Ariana 2020, TunisiaLaboratory of Biotechnology and Nuclear Technology, National Centre for Nuclear Sciences and Technologies (CNSTN), Sidi Thabet, Technopark, Ariana 2020, TunisiaLaboratory of Biotechnology and Nuclear Technology, National Centre for Nuclear Sciences and Technologies (CNSTN), Sidi Thabet, Technopark, Ariana 2020, TunisiaPGPR (Plant Growth Promoting Rhizobacteria) are used as biofertilizers and biological control agents against fungi. The objective of this work was to evaluate the antagonistic activities of some bacterial strains isolated from soil against four phytopathogenic fungal strains (<i>Fusarium graminearum</i>, <i>F. culmorum</i>, <i>Phytophthora</i> sp. and <i>Verticillium dahlia</i>). Two strains having an antagonist effect on fungi and displaying the maximum of plant growth promoting (PGP) traits were selected for further study and identified as <i>Bacillus subtilis</i> and <i>B. amyloliquefaciens</i> respectively. <i>In planta</i> assays demonstrated that the two <i>Bacillus</i> strains are able to enhance plant growth of two wheat cultivars in absence of nitrogen and protect them against <i>F. culmorum</i>. Pot experiments performed in a greenhouse showed that wheat plants inoculation with two bacterial strains reduce <i>F. culmorum</i> disease severity correlated with the accumulation of phenolic compounds and chlorophyll content. These could partly explain the effectiveness of these bacteria in protecting Tunisian durum wheat cultivars against <i>F. culmorum</i>. Application <i>B. amyloliquefaciens</i>, showed better protection than <i>B. subtilis</i> although the last one enhanced more the plant growth of two wheat cultivars in absence of fungus. Hence, combination of two bacterial strains could be a strategic approach to enhance plant growth and control plant diseases.https://www.mdpi.com/2076-2607/11/5/1165biocontrol<i>Fusarium culmorom</i>rhizobacteriaPGPR traitswheat |
| spellingShingle | Habiba Kouki Mouna Souihi Ilhem Saadouli Sabrine Balti Amira Ayed Nihed Majdoub Amor Mosbah Ismail Amri Yassine Mabrouk Biocontrol Potential of Some Rhizospheric Soil Bacterial Strains against <i>Fusarium culmorum</i> and Subsequent Effect on Growth of Two Tunisian Wheat Cultivars Microorganisms biocontrol <i>Fusarium culmorom</i> rhizobacteria PGPR traits wheat |
| title | Biocontrol Potential of Some Rhizospheric Soil Bacterial Strains against <i>Fusarium culmorum</i> and Subsequent Effect on Growth of Two Tunisian Wheat Cultivars |
| title_full | Biocontrol Potential of Some Rhizospheric Soil Bacterial Strains against <i>Fusarium culmorum</i> and Subsequent Effect on Growth of Two Tunisian Wheat Cultivars |
| title_fullStr | Biocontrol Potential of Some Rhizospheric Soil Bacterial Strains against <i>Fusarium culmorum</i> and Subsequent Effect on Growth of Two Tunisian Wheat Cultivars |
| title_full_unstemmed | Biocontrol Potential of Some Rhizospheric Soil Bacterial Strains against <i>Fusarium culmorum</i> and Subsequent Effect on Growth of Two Tunisian Wheat Cultivars |
| title_short | Biocontrol Potential of Some Rhizospheric Soil Bacterial Strains against <i>Fusarium culmorum</i> and Subsequent Effect on Growth of Two Tunisian Wheat Cultivars |
| title_sort | biocontrol potential of some rhizospheric soil bacterial strains against i fusarium culmorum i and subsequent effect on growth of two tunisian wheat cultivars |
| topic | biocontrol <i>Fusarium culmorom</i> rhizobacteria PGPR traits wheat |
| url | https://www.mdpi.com/2076-2607/11/5/1165 |
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