Genetic Authentication of Gardenia jasminoides Ellis var. grandiflora Nakai by Improved RAPD-Derived DNA Markers
The evergreen shrub, Gardenia jasminoides Ellis var. grandiflora Nakai is one of the most popular garden-plants, with significant ornamental importance. Here, we have cloned improved random amplified polymorphic DNA (RAPD) derived fragments into T-vector, and developed sequence-characterized amplifie...
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MDPI AG
2015-11-01
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| Series: | Molecules |
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| Online Access: | http://www.mdpi.com/1420-3049/20/11/19687 |
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| author | Zhiqiang Mei Boxu Zhou Chunli Wei Jingliang Cheng Saber Imani Hanchun Chen Junjiang Fu |
| author_facet | Zhiqiang Mei Boxu Zhou Chunli Wei Jingliang Cheng Saber Imani Hanchun Chen Junjiang Fu |
| author_sort | Zhiqiang Mei |
| collection | DOAJ |
| description | The evergreen shrub, Gardenia jasminoides Ellis var. grandiflora Nakai is one of the most popular garden-plants, with significant ornamental importance. Here, we have cloned improved random amplified polymorphic DNA (RAPD) derived fragments into T-vector, and developed sequence-characterized amplified region (SCAR) markers. These markers have been deposited in GenBank database with the accession numbers KP641310, KP641311, KP641312 and KP641313 respectively. The BLAST search of database confirmed the novelty of these markers. The four SCAR markers, namely ZZH11, ZZH31, ZZH41 and ZZH51 can specifically recognize the genetic materials of G. jasminoides from other plant species. Moreover, SCAR marker ZZH31 can be used to distinguish G. jasminoides Ellis var. grandiflora Nakai from other G. jasminoides on the market. Together, this study has developed four stably molecular SCAR markers by improved RAPD-derived DNA markers for the genetic identification and authentication, and for ecological conservation of medicinal and ornamental plant G. jasminoides. |
| first_indexed | 2024-12-22T13:09:19Z |
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| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-12-22T13:09:19Z |
| publishDate | 2015-11-01 |
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| series | Molecules |
| spelling | doaj.art-ff0debc0377949d492f545ede30086162022-12-21T18:24:48ZengMDPI AGMolecules1420-30492015-11-012011202192022910.3390/molecules201119687molecules201119687Genetic Authentication of Gardenia jasminoides Ellis var. grandiflora Nakai by Improved RAPD-Derived DNA MarkersZhiqiang Mei0Boxu Zhou1Chunli Wei2Jingliang Cheng3Saber Imani4Hanchun Chen5Junjiang Fu6Research Center for Preclinical Medicine, Sichuan Medical University, Luzhou 646000, ChinaResearch Center for Preclinical Medicine, Sichuan Medical University, Luzhou 646000, ChinaResearch Center for Preclinical Medicine, Sichuan Medical University, Luzhou 646000, ChinaResearch Center for Preclinical Medicine, Sichuan Medical University, Luzhou 646000, ChinaResearch Center for Preclinical Medicine, Sichuan Medical University, Luzhou 646000, ChinaDepartment of Biochemistry, School of Life Sciences & the State Key Laboratory of Medical Genetics, Central South University, Changsha 410013, ChinaResearch Center for Preclinical Medicine, Sichuan Medical University, Luzhou 646000, ChinaThe evergreen shrub, Gardenia jasminoides Ellis var. grandiflora Nakai is one of the most popular garden-plants, with significant ornamental importance. Here, we have cloned improved random amplified polymorphic DNA (RAPD) derived fragments into T-vector, and developed sequence-characterized amplified region (SCAR) markers. These markers have been deposited in GenBank database with the accession numbers KP641310, KP641311, KP641312 and KP641313 respectively. The BLAST search of database confirmed the novelty of these markers. The four SCAR markers, namely ZZH11, ZZH31, ZZH41 and ZZH51 can specifically recognize the genetic materials of G. jasminoides from other plant species. Moreover, SCAR marker ZZH31 can be used to distinguish G. jasminoides Ellis var. grandiflora Nakai from other G. jasminoides on the market. Together, this study has developed four stably molecular SCAR markers by improved RAPD-derived DNA markers for the genetic identification and authentication, and for ecological conservation of medicinal and ornamental plant G. jasminoides.http://www.mdpi.com/1420-3049/20/11/19687G. jasminoides Ellis var. grandiflora NakaiGardenia jasminoidesrandom amplified polymorphic DNAsequence-characterized amplified regioncloninggenetic authentication |
| spellingShingle | Zhiqiang Mei Boxu Zhou Chunli Wei Jingliang Cheng Saber Imani Hanchun Chen Junjiang Fu Genetic Authentication of Gardenia jasminoides Ellis var. grandiflora Nakai by Improved RAPD-Derived DNA Markers Molecules G. jasminoides Ellis var. grandiflora Nakai Gardenia jasminoides random amplified polymorphic DNA sequence-characterized amplified region cloning genetic authentication |
| title | Genetic Authentication of Gardenia jasminoides Ellis var. grandiflora Nakai by Improved RAPD-Derived DNA Markers |
| title_full | Genetic Authentication of Gardenia jasminoides Ellis var. grandiflora Nakai by Improved RAPD-Derived DNA Markers |
| title_fullStr | Genetic Authentication of Gardenia jasminoides Ellis var. grandiflora Nakai by Improved RAPD-Derived DNA Markers |
| title_full_unstemmed | Genetic Authentication of Gardenia jasminoides Ellis var. grandiflora Nakai by Improved RAPD-Derived DNA Markers |
| title_short | Genetic Authentication of Gardenia jasminoides Ellis var. grandiflora Nakai by Improved RAPD-Derived DNA Markers |
| title_sort | genetic authentication of gardenia jasminoides ellis var grandiflora nakai by improved rapd derived dna markers |
| topic | G. jasminoides Ellis var. grandiflora Nakai Gardenia jasminoides random amplified polymorphic DNA sequence-characterized amplified region cloning genetic authentication |
| url | http://www.mdpi.com/1420-3049/20/11/19687 |
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