Effect of bioactive aldehydes on gelatin properties
Bioactive aldehydes are among main factors of proteins postsynthetic modifications, which are the cause and consequence of many diseases. Comparative study of some aldehydes modifying action on gelatin was carried out in vitro. Gelatin samples (20 mM) were incubated with: ribose, deoxyribose, glyoxa...
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| Format: | Article |
| Language: | English |
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National Academy of Sciences of Ukraine, Palladin Institute of Biochemistry
2015-04-01
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| Series: | The Ukrainian Biochemical Journal |
| Subjects: | |
| Online Access: | http://ukrbiochemjournal.org/wp-content/uploads/2015/05/Krysyuk_2_15.pdf |
| _version_ | 1827609331490619392 |
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| author | I. P. Krysyuk N. D. Dzvonkevych T. T. Volodina N. N. Popova S. G. Shandrenko |
| author_facet | I. P. Krysyuk N. D. Dzvonkevych T. T. Volodina N. N. Popova S. G. Shandrenko |
| author_sort | I. P. Krysyuk |
| collection | DOAJ |
| description | Bioactive aldehydes are among main factors of proteins postsynthetic modifications, which are the cause and consequence of many diseases. Comparative study of some aldehydes modifying action on gelatin was carried out in vitro. Gelatin samples (20 mM) were incubated with: ribose, deoxyribose, glyoxal, methylglyoxal, formaldehyde, acrolein (20 mM each) and their combinations in 0.1 M Na-phosphate buffer (pH 7.4) containing 0.02% sodium azide at 37 °C in the dark for 30 days. We investigated the fluorescent properties of these samples and their molecular weight distribution by electrophoresis. It has been revealed that formed adducts had different fluorescence spectra. According to fluorescence intensity these aldehydes were put in order: formaldehyde < methylglyoxal < acrolein < ribose < deoxyribose < glyoxal. The electrophoresis results showed fragments of gelatin molecular weight redistribution. By this index, the aldehydes rating was as follows: ribose < deoxyribose < acrolein < glyoxal < formaldehyde < methylglyoxal. Comparison of these two ratings indicates that aldehydes with a lower ability to form fluorescent adducts have higher ability to form intermolecular crosslinks. Therefore, the traditional clinical fluorescent test of a patients’ skin surface for collagen crosslinks determination has to be verified by other tests for proteins postsynthetic modifications. |
| first_indexed | 2024-03-09T07:30:51Z |
| format | Article |
| id | doaj.art-ff0fce8168624329954e302cc647b673 |
| institution | Directory Open Access Journal |
| issn | 2409-4943 2413-5003 |
| language | English |
| last_indexed | 2024-03-09T07:30:51Z |
| publishDate | 2015-04-01 |
| publisher | National Academy of Sciences of Ukraine, Palladin Institute of Biochemistry |
| record_format | Article |
| series | The Ukrainian Biochemical Journal |
| spelling | doaj.art-ff0fce8168624329954e302cc647b6732023-12-03T06:27:16ZengNational Academy of Sciences of Ukraine, Palladin Institute of BiochemistryThe Ukrainian Biochemical Journal2409-49432413-50032015-04-0187211312110.15407/ubj87.02.113Effect of bioactive aldehydes on gelatin propertiesI. P. Krysyuk0N. D. Dzvonkevych1T. T. Volodina2N. N. Popova3S. G. Shandrenko4Palladin Institute of Biochemistry, National Academy of Sciences of Ukraine, KyivPalladin Institute of Biochemistry, National Academy of Sciences of Ukraine, KyivPalladin Institute of Biochemistry, National Academy of Sciences of Ukraine, KyivPalladin Institute of Biochemistry, National Academy of Sciences of Ukraine, KyivPalladin Institute of Biochemistry, National Academy of Sciences of Ukraine, KyivBioactive aldehydes are among main factors of proteins postsynthetic modifications, which are the cause and consequence of many diseases. Comparative study of some aldehydes modifying action on gelatin was carried out in vitro. Gelatin samples (20 mM) were incubated with: ribose, deoxyribose, glyoxal, methylglyoxal, formaldehyde, acrolein (20 mM each) and their combinations in 0.1 M Na-phosphate buffer (pH 7.4) containing 0.02% sodium azide at 37 °C in the dark for 30 days. We investigated the fluorescent properties of these samples and their molecular weight distribution by electrophoresis. It has been revealed that formed adducts had different fluorescence spectra. According to fluorescence intensity these aldehydes were put in order: formaldehyde < methylglyoxal < acrolein < ribose < deoxyribose < glyoxal. The electrophoresis results showed fragments of gelatin molecular weight redistribution. By this index, the aldehydes rating was as follows: ribose < deoxyribose < acrolein < glyoxal < formaldehyde < methylglyoxal. Comparison of these two ratings indicates that aldehydes with a lower ability to form fluorescent adducts have higher ability to form intermolecular crosslinks. Therefore, the traditional clinical fluorescent test of a patients’ skin surface for collagen crosslinks determination has to be verified by other tests for proteins postsynthetic modifications.http://ukrbiochemjournal.org/wp-content/uploads/2015/05/Krysyuk_2_15.pdfadductsaldehydesfluorescencegelatinprotein modification |
| spellingShingle | I. P. Krysyuk N. D. Dzvonkevych T. T. Volodina N. N. Popova S. G. Shandrenko Effect of bioactive aldehydes on gelatin properties The Ukrainian Biochemical Journal adducts aldehydes fluorescence gelatin protein modification |
| title | Effect of bioactive aldehydes on gelatin properties |
| title_full | Effect of bioactive aldehydes on gelatin properties |
| title_fullStr | Effect of bioactive aldehydes on gelatin properties |
| title_full_unstemmed | Effect of bioactive aldehydes on gelatin properties |
| title_short | Effect of bioactive aldehydes on gelatin properties |
| title_sort | effect of bioactive aldehydes on gelatin properties |
| topic | adducts aldehydes fluorescence gelatin protein modification |
| url | http://ukrbiochemjournal.org/wp-content/uploads/2015/05/Krysyuk_2_15.pdf |
| work_keys_str_mv | AT ipkrysyuk effectofbioactivealdehydesongelatinproperties AT nddzvonkevych effectofbioactivealdehydesongelatinproperties AT ttvolodina effectofbioactivealdehydesongelatinproperties AT nnpopova effectofbioactivealdehydesongelatinproperties AT sgshandrenko effectofbioactivealdehydesongelatinproperties |