In Vitro Organogenesis of a Slipper Orchid, Paphiopedilum ‘Alma Gavaert’
The aim of the present study was to improve the regeneration efficiency of callus lines in a slipper orchid, Paphiopedilum ‘Alma Gavaert’. Three kinds of vegetative tissues, root, stem and leaf segments, were used as explants to induce callogenesis; out of these, only root explants formed callus and...
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| Format: | Article |
| Language: | English |
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Society of Land Measurements and Cadastre from Transylvania (SMTCT)
2018-12-01
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| Series: | Notulae Scientia Biologicae |
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| Online Access: | https://www.notulaebiologicae.ro/index.php/nsb/article/view/10368 |
| _version_ | 1818010618078167040 |
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| author | Jen-Tsung CHEN |
| author_facet | Jen-Tsung CHEN |
| author_sort | Jen-Tsung CHEN |
| collection | DOAJ |
| description | The aim of the present study was to improve the regeneration efficiency of callus lines in a slipper orchid, Paphiopedilum ‘Alma Gavaert’. Three kinds of vegetative tissues, root, stem and leaf segments, were used as explants to induce callogenesis; out of these, only root explants formed callus and was subcultured in the presence of 5 mg/L dicamba and 5 mg/L 2,4-D combined with 1 or 2 mg/L TDZ. The resulting four callus lines, assigned as 5Di1T, 5Di2T, 5D1T and 5D2T, respectively, were used to test the effect of NAA to BA ratios on re-differentiation, wherein the highest number of shoots (approximately 2 shoots/0.1 g callus clump) were obtained in callus line 5D2T at ratios of 0.001 and 0.002. A largely improvement of shoot regeneration efficiency was obtained by continuous selection of callus lines which derived from different explant positions. Eventually, six callus lines, including 5D2T-T6-G5 to 5D2T-T6-G10, were able to produce approximately 10 times of shoots per callus clump when compared with the parental callus line 5D2T. |
| first_indexed | 2024-04-14T05:57:34Z |
| format | Article |
| id | doaj.art-ff126a535af344c3a2985f013ae68545 |
| institution | Directory Open Access Journal |
| issn | 2067-3205 2067-3264 |
| language | English |
| last_indexed | 2024-04-14T05:57:34Z |
| publishDate | 2018-12-01 |
| publisher | Society of Land Measurements and Cadastre from Transylvania (SMTCT) |
| record_format | Article |
| series | Notulae Scientia Biologicae |
| spelling | doaj.art-ff126a535af344c3a2985f013ae685452022-12-22T02:08:53ZengSociety of Land Measurements and Cadastre from Transylvania (SMTCT)Notulae Scientia Biologicae2067-32052067-32642018-12-0110460761310.15835/nsb104103688431In Vitro Organogenesis of a Slipper Orchid, Paphiopedilum ‘Alma Gavaert’Jen-Tsung CHEN0National University of Kaohsiung, Department of Life Sciences, Kaohsiung University Rd., No. 700, Kaohsiung 811, TaiwanThe aim of the present study was to improve the regeneration efficiency of callus lines in a slipper orchid, Paphiopedilum ‘Alma Gavaert’. Three kinds of vegetative tissues, root, stem and leaf segments, were used as explants to induce callogenesis; out of these, only root explants formed callus and was subcultured in the presence of 5 mg/L dicamba and 5 mg/L 2,4-D combined with 1 or 2 mg/L TDZ. The resulting four callus lines, assigned as 5Di1T, 5Di2T, 5D1T and 5D2T, respectively, were used to test the effect of NAA to BA ratios on re-differentiation, wherein the highest number of shoots (approximately 2 shoots/0.1 g callus clump) were obtained in callus line 5D2T at ratios of 0.001 and 0.002. A largely improvement of shoot regeneration efficiency was obtained by continuous selection of callus lines which derived from different explant positions. Eventually, six callus lines, including 5D2T-T6-G5 to 5D2T-T6-G10, were able to produce approximately 10 times of shoots per callus clump when compared with the parental callus line 5D2T.https://www.notulaebiologicae.ro/index.php/nsb/article/view/10368callusheterogeneityorganogenesisshoot formationslipper orchid |
| spellingShingle | Jen-Tsung CHEN In Vitro Organogenesis of a Slipper Orchid, Paphiopedilum ‘Alma Gavaert’ Notulae Scientia Biologicae callus heterogeneity organogenesis shoot formation slipper orchid |
| title | In Vitro Organogenesis of a Slipper Orchid, Paphiopedilum ‘Alma Gavaert’ |
| title_full | In Vitro Organogenesis of a Slipper Orchid, Paphiopedilum ‘Alma Gavaert’ |
| title_fullStr | In Vitro Organogenesis of a Slipper Orchid, Paphiopedilum ‘Alma Gavaert’ |
| title_full_unstemmed | In Vitro Organogenesis of a Slipper Orchid, Paphiopedilum ‘Alma Gavaert’ |
| title_short | In Vitro Organogenesis of a Slipper Orchid, Paphiopedilum ‘Alma Gavaert’ |
| title_sort | in vitro organogenesis of a slipper orchid paphiopedilum alma gavaert |
| topic | callus heterogeneity organogenesis shoot formation slipper orchid |
| url | https://www.notulaebiologicae.ro/index.php/nsb/article/view/10368 |
| work_keys_str_mv | AT jentsungchen invitroorganogenesisofaslipperorchidpaphiopedilumalmagavaert |