Free Rhodium (II) citrate and rhodium (II) citrate magnetic carriers as potential strategies for breast cancer therapy
<p>Abstract</p> <p>Background</p> <p>Rhodium (II) citrate (Rh<sub>2</sub>(H<sub>2</sub>cit)<sub>4</sub>) has significant antitumor, cytotoxic, and cytostatic activity on Ehrlich ascite tumor. Although toxic to normal cells, its lower...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
BMC
2011-03-01
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| Series: | Journal of Nanobiotechnology |
| Online Access: | http://www.jnanobiotechnology.com/content/9/1/11 |
| _version_ | 1811305364537212928 |
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| author | de Souza Aparecido R Tedesco Antônio C Simioni Andreza R da Silva Izabel CR Lourenço Luiza HM Oliveira Ricardo GS Peixoto Raphael CA Nunes Eloiza S Carneiro Marcella LB Lacava Zulmira GM Báo Sônia N |
| author_facet | de Souza Aparecido R Tedesco Antônio C Simioni Andreza R da Silva Izabel CR Lourenço Luiza HM Oliveira Ricardo GS Peixoto Raphael CA Nunes Eloiza S Carneiro Marcella LB Lacava Zulmira GM Báo Sônia N |
| author_sort | de Souza Aparecido R |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>Rhodium (II) citrate (Rh<sub>2</sub>(H<sub>2</sub>cit)<sub>4</sub>) has significant antitumor, cytotoxic, and cytostatic activity on Ehrlich ascite tumor. Although toxic to normal cells, its lower toxicity when compared to carboxylate analogues of rhodium (II) indicates Rh<sub>2</sub>(H<sub>2</sub>cit)<sub>4 </sub>as a promising agent for chemotherapy. Nevertheless, few studies have been performed to explore this potential. Superparamagnetic particles of iron oxide (SPIOs) represent an attractive platform as carriers in drug delivery systems (DDS) because they can present greater specificity to tumor cells than normal cells. Thus, the association between Rh<sub>2</sub>(H<sub>2</sub>cit)<sub>4 </sub>and SPIOs can represent a strategy to enhance the former's therapeutic action. In this work, we report the cytotoxicity of free rhodium (II) citrate (Rh<sub>2</sub>(H<sub>2</sub>cit)<sub>4</sub>) and rhodium (II) citrate-loaded maghemite nanoparticles or magnetoliposomes, used as drug delivery systems, on both normal and carcinoma breast cell cultures.</p> <p>Results</p> <p>Treatment with free Rh<sub>2</sub>(H<sub>2</sub>cit)<sub>4 </sub>induced cytotoxicity that was dependent on dose, time, and cell line. The IC<sub>50 </sub>values showed that this effect was more intense on breast normal cells (MCF-10A) than on breast carcinoma cells (MCF-7 and 4T1). However, the treatment with 50 μM Rh<sub>2</sub>(H<sub>2</sub>cit)<sub>4</sub>-loaded maghemite nanoparticles (Magh-Rh<sub>2</sub>(H<sub>2</sub>cit)<sub>4</sub>) and Rh<sub>2</sub>(H<sub>2</sub>cit)<sub>4</sub>-loaded magnetoliposomes (Lip-Magh-Rh<sub>2</sub>(H<sub>2</sub>cit)<sub>4</sub>) induced a higher cytotoxicity on MCF-7 and 4T1 than on MCF-10A (p < 0.05). These treatments enhanced cytotoxicity up to 4.6 times. These cytotoxic effects, induced by free Rh<sub>2</sub>(H<sub>2</sub>cit)<sub>4</sub>, were evidenced by morphological alterations such as nuclear fragmentation, membrane blebbing and phosphatidylserine exposure, reduction of actin filaments, mitochondrial condensation and an increase in number of vacuoles, suggesting that Rh<sub>2</sub>(H<sub>2</sub>cit)<sub>4 </sub>induces cell death by apoptosis.</p> <p>Conclusions</p> <p>The treatment with rhodium (II) citrate-loaded maghemite nanoparticles and magnetoliposomes induced more specific cytotoxicity on breast carcinoma cells than on breast normal cells, which is the opposite of the results observed with free Rh<sub>2</sub>(H<sub>2</sub>cit)<sub>4 </sub>treatment. Thus, magnetic nanoparticles represent an attractive platform as carriers in Rh<sub>2</sub>(H<sub>2</sub>cit)<sub>4 </sub>delivery systems, since they can act preferentially in tumor cells. Therefore, these nanopaticulate systems may be explored as a potential tool for chemotherapy drug development.</p> |
| first_indexed | 2024-04-13T08:24:08Z |
| format | Article |
| id | doaj.art-ff130c8da42f441690d690c6cb051313 |
| institution | Directory Open Access Journal |
| issn | 1477-3155 |
| language | English |
| last_indexed | 2024-04-13T08:24:08Z |
| publishDate | 2011-03-01 |
| publisher | BMC |
| record_format | Article |
| series | Journal of Nanobiotechnology |
| spelling | doaj.art-ff130c8da42f441690d690c6cb0513132022-12-22T02:54:33ZengBMCJournal of Nanobiotechnology1477-31552011-03-01911110.1186/1477-3155-9-11Free Rhodium (II) citrate and rhodium (II) citrate magnetic carriers as potential strategies for breast cancer therapyde Souza Aparecido RTedesco Antônio CSimioni Andreza Rda Silva Izabel CRLourenço Luiza HMOliveira Ricardo GSPeixoto Raphael CANunes Eloiza SCarneiro Marcella LBLacava Zulmira GMBáo Sônia N<p>Abstract</p> <p>Background</p> <p>Rhodium (II) citrate (Rh<sub>2</sub>(H<sub>2</sub>cit)<sub>4</sub>) has significant antitumor, cytotoxic, and cytostatic activity on Ehrlich ascite tumor. Although toxic to normal cells, its lower toxicity when compared to carboxylate analogues of rhodium (II) indicates Rh<sub>2</sub>(H<sub>2</sub>cit)<sub>4 </sub>as a promising agent for chemotherapy. Nevertheless, few studies have been performed to explore this potential. Superparamagnetic particles of iron oxide (SPIOs) represent an attractive platform as carriers in drug delivery systems (DDS) because they can present greater specificity to tumor cells than normal cells. Thus, the association between Rh<sub>2</sub>(H<sub>2</sub>cit)<sub>4 </sub>and SPIOs can represent a strategy to enhance the former's therapeutic action. In this work, we report the cytotoxicity of free rhodium (II) citrate (Rh<sub>2</sub>(H<sub>2</sub>cit)<sub>4</sub>) and rhodium (II) citrate-loaded maghemite nanoparticles or magnetoliposomes, used as drug delivery systems, on both normal and carcinoma breast cell cultures.</p> <p>Results</p> <p>Treatment with free Rh<sub>2</sub>(H<sub>2</sub>cit)<sub>4 </sub>induced cytotoxicity that was dependent on dose, time, and cell line. The IC<sub>50 </sub>values showed that this effect was more intense on breast normal cells (MCF-10A) than on breast carcinoma cells (MCF-7 and 4T1). However, the treatment with 50 μM Rh<sub>2</sub>(H<sub>2</sub>cit)<sub>4</sub>-loaded maghemite nanoparticles (Magh-Rh<sub>2</sub>(H<sub>2</sub>cit)<sub>4</sub>) and Rh<sub>2</sub>(H<sub>2</sub>cit)<sub>4</sub>-loaded magnetoliposomes (Lip-Magh-Rh<sub>2</sub>(H<sub>2</sub>cit)<sub>4</sub>) induced a higher cytotoxicity on MCF-7 and 4T1 than on MCF-10A (p < 0.05). These treatments enhanced cytotoxicity up to 4.6 times. These cytotoxic effects, induced by free Rh<sub>2</sub>(H<sub>2</sub>cit)<sub>4</sub>, were evidenced by morphological alterations such as nuclear fragmentation, membrane blebbing and phosphatidylserine exposure, reduction of actin filaments, mitochondrial condensation and an increase in number of vacuoles, suggesting that Rh<sub>2</sub>(H<sub>2</sub>cit)<sub>4 </sub>induces cell death by apoptosis.</p> <p>Conclusions</p> <p>The treatment with rhodium (II) citrate-loaded maghemite nanoparticles and magnetoliposomes induced more specific cytotoxicity on breast carcinoma cells than on breast normal cells, which is the opposite of the results observed with free Rh<sub>2</sub>(H<sub>2</sub>cit)<sub>4 </sub>treatment. Thus, magnetic nanoparticles represent an attractive platform as carriers in Rh<sub>2</sub>(H<sub>2</sub>cit)<sub>4 </sub>delivery systems, since they can act preferentially in tumor cells. Therefore, these nanopaticulate systems may be explored as a potential tool for chemotherapy drug development.</p>http://www.jnanobiotechnology.com/content/9/1/11 |
| spellingShingle | de Souza Aparecido R Tedesco Antônio C Simioni Andreza R da Silva Izabel CR Lourenço Luiza HM Oliveira Ricardo GS Peixoto Raphael CA Nunes Eloiza S Carneiro Marcella LB Lacava Zulmira GM Báo Sônia N Free Rhodium (II) citrate and rhodium (II) citrate magnetic carriers as potential strategies for breast cancer therapy Journal of Nanobiotechnology |
| title | Free Rhodium (II) citrate and rhodium (II) citrate magnetic carriers as potential strategies for breast cancer therapy |
| title_full | Free Rhodium (II) citrate and rhodium (II) citrate magnetic carriers as potential strategies for breast cancer therapy |
| title_fullStr | Free Rhodium (II) citrate and rhodium (II) citrate magnetic carriers as potential strategies for breast cancer therapy |
| title_full_unstemmed | Free Rhodium (II) citrate and rhodium (II) citrate magnetic carriers as potential strategies for breast cancer therapy |
| title_short | Free Rhodium (II) citrate and rhodium (II) citrate magnetic carriers as potential strategies for breast cancer therapy |
| title_sort | free rhodium ii citrate and rhodium ii citrate magnetic carriers as potential strategies for breast cancer therapy |
| url | http://www.jnanobiotechnology.com/content/9/1/11 |
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