Andrographolide induces cycle arrest at G2/M phase and intrinsic apoptosis in gastric carcinoma cells(穿心莲内酯诱导胃癌细胞周期抑制和内源性凋亡)

Andrographolide induces cycle arrest at G2/M phase and intrinsic apoptosis in gastric carcinoma cells(穿心莲内酯诱导胃癌细胞周期抑制和内源性凋亡)

研究了穿心莲内酯诱导胃癌细胞SGC-7901生长抑制、周期阻滞与细胞凋亡的作用机制.用梯度浓度的穿心莲内酯作用于SGC-7901细胞，以MTT法检测药物对细胞的生长抑制情况；以流式细胞技术检测SGC-7901的周期分布状况、胞内的ROS水平以及线粒体膜电位的变化；以Annexin V-FITC/PI双染法检测细胞凋亡的水平；以Western blot检测不同加药浓度处理后的细胞内相关蛋白水平的变化.可知穿心莲内酯对SGC-7901细胞具有时间依赖和浓度依赖的抑制效果，48 h的IC50约为24 µg • mL-1；并且能够有效抑制G2/M期的周期进展，随后诱发内源性凋亡途径；与此同时，细胞内的...

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Main Authors: LIUQizhang(刘奇章), GONGXingguo(龚兴国)
Format: Article
Language:zho
Published: Zhejiang University Press 2017-07-01
Series:Zhejiang Daxue xuebao. Lixue ban
Subjects:
穿心莲内酯
胃癌
凋亡诱导
周期阻滞
Online Access:https://doi.org/10.3785/j.issn.1008-9497.2017.04.011
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author LIUQizhang(刘奇章)
GONGXingguo(龚兴国)
author_facet LIUQizhang(刘奇章)
GONGXingguo(龚兴国)
author_sort LIUQizhang(刘奇章)
collection DOAJ
description 研究了穿心莲内酯诱导胃癌细胞SGC-7901生长抑制、周期阻滞与细胞凋亡的作用机制.用梯度浓度的穿心莲内酯作用于SGC-7901细胞，以MTT法检测药物对细胞的生长抑制情况；以流式细胞技术检测SGC-7901的周期分布状况、胞内的ROS水平以及线粒体膜电位的变化；以Annexin V-FITC/PI双染法检测细胞凋亡的水平；以Western blot检测不同加药浓度处理后的细胞内相关蛋白水平的变化.可知穿心莲内酯对SGC-7901细胞具有时间依赖和浓度依赖的抑制效果，48 h的IC50约为24 µg • mL-1；并且能够有效抑制G2/M期的周期进展，随后诱发内源性凋亡途径；与此同时，细胞内的ROS水平上升，线粒体膜电位下降；再加入NADPH之后，细胞内的GSH水平有所回升，周期阻滞得到缓解.细胞内的p53、p-CDC2、cleaved caspase-3/9、Bax等上调；bcl-2等水平下调.穿心莲内酯是通过上调胞内ROS水平并使线粒体膜电位崩溃而诱发G2/M期的周期阻滞与内源性凋亡，最终抑制SGC-7901细胞的生长.
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spelling doaj.art-ff180b78ee5a4355883a94a62f6ed6e92024-03-29T01:58:37ZzhoZhejiang University PressZhejiang Daxue xuebao. Lixue ban1008-94972017-07-0144445646310.3785/j.issn.1008-9497.2017.04.011Andrographolide induces cycle arrest at G2/M phase and intrinsic apoptosis in gastric carcinoma cells(穿心莲内酯诱导胃癌细胞周期抑制和内源性凋亡)LIUQizhang(刘奇章)0https://orcid.org/0000-0002-4043-1783GONGXingguo(龚兴国)https://orcid.org/0000-0003-1206-5154Institute of Biochemistry, College of life Sciences, Zhejiang University, Hangzhou 310058, China(浙江大学生命科学学院生物化学研究所，浙江 杭州 310058)研究了穿心莲内酯诱导胃癌细胞SGC-7901生长抑制、周期阻滞与细胞凋亡的作用机制.用梯度浓度的穿心莲内酯作用于SGC-7901细胞，以MTT法检测药物对细胞的生长抑制情况；以流式细胞技术检测SGC-7901的周期分布状况、胞内的ROS水平以及线粒体膜电位的变化；以Annexin V-FITC/PI双染法检测细胞凋亡的水平；以Western blot检测不同加药浓度处理后的细胞内相关蛋白水平的变化.可知穿心莲内酯对SGC-7901细胞具有时间依赖和浓度依赖的抑制效果，48 h的IC50约为24 µg • mL-1；并且能够有效抑制G2/M期的周期进展，随后诱发内源性凋亡途径；与此同时，细胞内的ROS水平上升，线粒体膜电位下降；再加入NADPH之后，细胞内的GSH水平有所回升，周期阻滞得到缓解.细胞内的p53、p-CDC2、cleaved caspase-3/9、Bax等上调；bcl-2等水平下调.穿心莲内酯是通过上调胞内ROS水平并使线粒体膜电位崩溃而诱发G2/M期的周期阻滞与内源性凋亡，最终抑制SGC-7901细胞的生长.https://doi.org/10.3785/j.issn.1008-9497.2017.04.011穿心莲内酯胃癌凋亡诱导周期阻滞
spellingShingle LIUQizhang(刘奇章)
GONGXingguo(龚兴国)
Andrographolide induces cycle arrest at G2/M phase and intrinsic apoptosis in gastric carcinoma cells(穿心莲内酯诱导胃癌细胞周期抑制和内源性凋亡)
Zhejiang Daxue xuebao. Lixue ban
穿心莲内酯
胃癌
凋亡诱导
周期阻滞
title Andrographolide induces cycle arrest at G2/M phase and intrinsic apoptosis in gastric carcinoma cells(穿心莲内酯诱导胃癌细胞周期抑制和内源性凋亡)
title_full Andrographolide induces cycle arrest at G2/M phase and intrinsic apoptosis in gastric carcinoma cells(穿心莲内酯诱导胃癌细胞周期抑制和内源性凋亡)
title_fullStr Andrographolide induces cycle arrest at G2/M phase and intrinsic apoptosis in gastric carcinoma cells(穿心莲内酯诱导胃癌细胞周期抑制和内源性凋亡)
title_full_unstemmed Andrographolide induces cycle arrest at G2/M phase and intrinsic apoptosis in gastric carcinoma cells(穿心莲内酯诱导胃癌细胞周期抑制和内源性凋亡)
title_short Andrographolide induces cycle arrest at G2/M phase and intrinsic apoptosis in gastric carcinoma cells(穿心莲内酯诱导胃癌细胞周期抑制和内源性凋亡)
title_sort andrographolide induces cycle arrest at g2 m phase and intrinsic apoptosis in gastric carcinoma cells 穿心莲内酯诱导胃癌细胞周期抑制和内源性凋亡
topic 穿心莲内酯
胃癌
凋亡诱导
周期阻滞
url https://doi.org/10.3785/j.issn.1008-9497.2017.04.011
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AT gongxingguogōngxìngguó andrographolideinducescyclearrestatg2mphaseandintrinsicapoptosisingastriccarcinomacellschuānxīnliánnèizhǐyòudǎowèiáixìbāozhōuqīyìzhìhénèiyuánxìngdiāowáng

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