| Summary: | The spatiotemporal distributions of microbes in soil by different methods could affect the efficacy of the microbes to reduce the soil hydraulic conductivity. In this study, the specimens of bio-mediated sands were prepared using three different methods, i.e. injecting, mixing, and pouring a given microbial solution onto compacted sand specimens. The hydraulic conductivity was measured by constant-head tests, while any soil microstructural changes due to addition of the microbes were observed by scanning electron microscope (SEM) and mercury intrusion porosimetry (MIP) tests. The amount of dextran concentration produced by microbes in each type of specimen was quantified by a refractometer. Results show that dextran production increased exponentially after 5–7 d of microbial settling with the supply of culture medium. The injection and mixing methods resulted in a similar amount and uniform distribution of dextran in the specimens. The pouring method, however, produced a nonuniform distribution, with a higher concentration near the specimen surface. As the supply of culture medium discontinued, the dextran content near the surface produced by the pouring method decreased dramatically due to high competition for nutrients with foreign colonies. Average dextran concentration was negatively and correlated with hydraulic conductivity of bio-mediated soils exponentially, due to the clogging of large soil pores by dextran. The hydraulic conductivity of the injection and mixing cases did not change significantly when the supply of culture medium was absent.
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