Effects of blood flow restriction on muscle size and gene expression in muscle during immobilization: A pilot study
Abstract Purpose Muscle mass is known to rapidly decrease with muscle disuse. Previous reports suggest that repetitive blood flow restriction (BFR) mitigates the reduction of muscle mass with disuse. However, the effects of BFR on muscle atrophy and gene expression levels in muscle during cast immob...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2020-07-01
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| Series: | Physiological Reports |
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| Online Access: | https://doi.org/10.14814/phy2.14516 |
| _version_ | 1818085652905852928 |
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| author | Saori Kakehi Yoshifumi Tamura Atsushi Kubota Kageumi Takeno Minako Kawaguchi Keishoku Sakuraba Ryuzo Kawamori Hirotaka Watada |
| author_facet | Saori Kakehi Yoshifumi Tamura Atsushi Kubota Kageumi Takeno Minako Kawaguchi Keishoku Sakuraba Ryuzo Kawamori Hirotaka Watada |
| author_sort | Saori Kakehi |
| collection | DOAJ |
| description | Abstract Purpose Muscle mass is known to rapidly decrease with muscle disuse. Previous reports suggest that repetitive blood flow restriction (BFR) mitigates the reduction of muscle mass with disuse. However, the effects of BFR on muscle atrophy and gene expression levels in muscle during cast immobilization have not been clarified. Methods To investigate the effect of BFR on muscle atrophy and gene expression levels during cast immobilization in humans, we recruited 10 healthy males who were randomly divided into the control and BFR treatment groups. All subjects were immobilized with a cast for 14 days. BFR treatment was conducted only in the BFR group. We evaluated cross sectional area (CSA) of thigh muscles by magnetic resonance imaging before and 14 days after cast immobilization. A percutaneous biopsy of the vastus lateralis muscle (VL) was performed before and 1, 7, and 14 days after cast immobilization. Expression of genes related to muscle atrophy and synthesis were evaluated using real‐time PCR. Results The CSA of the VL and the thigh flexor muscles were significantly decreased in both groups; however, percent decrease in CSA was significantly smaller in the BFR group compared with the control group. In two‐way repeated ANOVA analysis, the time × treatment interaction in gene expression of the muscle‐specific ubiquitin ligases muscle ring finger 1 (MuRF1) was significant, and elevated MURF1 expression level by cast immobilization was seemed to be suppressed by the BFR treatment. Conclusion BFR treatment may prevent reduced VL and thigh flexor muscles and increased MuRF1 expression level during cast immobilization. Further study is required to confirm these results. |
| first_indexed | 2024-12-10T20:13:12Z |
| format | Article |
| id | doaj.art-ff1f58ecccc246febb82313dbe066476 |
| institution | Directory Open Access Journal |
| issn | 2051-817X |
| language | English |
| last_indexed | 2024-12-10T20:13:12Z |
| publishDate | 2020-07-01 |
| publisher | Wiley |
| record_format | Article |
| series | Physiological Reports |
| spelling | doaj.art-ff1f58ecccc246febb82313dbe0664762022-12-22T01:35:16ZengWileyPhysiological Reports2051-817X2020-07-01814n/an/a10.14814/phy2.14516Effects of blood flow restriction on muscle size and gene expression in muscle during immobilization: A pilot studySaori Kakehi0Yoshifumi Tamura1Atsushi Kubota2Kageumi Takeno3Minako Kawaguchi4Keishoku Sakuraba5Ryuzo Kawamori6Hirotaka Watada7Department of Metabolism & Endocrinology Juntendo University Graduate School of Medicine Tokyo JapanDepartment of Metabolism & Endocrinology Juntendo University Graduate School of Medicine Tokyo JapanDepartment of Sports Medicine Juntendo University Graduate School of Health and Sports Science Chiba JapanDepartment of Metabolism & Endocrinology Juntendo University Graduate School of Medicine Tokyo JapanDepartment of Metabolism & Endocrinology Juntendo University Graduate School of Medicine Tokyo JapanDepartment of Sports Medicine Juntendo University Graduate School of Health and Sports Science Chiba JapanDepartment of Metabolism & Endocrinology Juntendo University Graduate School of Medicine Tokyo JapanDepartment of Metabolism & Endocrinology Juntendo University Graduate School of Medicine Tokyo JapanAbstract Purpose Muscle mass is known to rapidly decrease with muscle disuse. Previous reports suggest that repetitive blood flow restriction (BFR) mitigates the reduction of muscle mass with disuse. However, the effects of BFR on muscle atrophy and gene expression levels in muscle during cast immobilization have not been clarified. Methods To investigate the effect of BFR on muscle atrophy and gene expression levels during cast immobilization in humans, we recruited 10 healthy males who were randomly divided into the control and BFR treatment groups. All subjects were immobilized with a cast for 14 days. BFR treatment was conducted only in the BFR group. We evaluated cross sectional area (CSA) of thigh muscles by magnetic resonance imaging before and 14 days after cast immobilization. A percutaneous biopsy of the vastus lateralis muscle (VL) was performed before and 1, 7, and 14 days after cast immobilization. Expression of genes related to muscle atrophy and synthesis were evaluated using real‐time PCR. Results The CSA of the VL and the thigh flexor muscles were significantly decreased in both groups; however, percent decrease in CSA was significantly smaller in the BFR group compared with the control group. In two‐way repeated ANOVA analysis, the time × treatment interaction in gene expression of the muscle‐specific ubiquitin ligases muscle ring finger 1 (MuRF1) was significant, and elevated MURF1 expression level by cast immobilization was seemed to be suppressed by the BFR treatment. Conclusion BFR treatment may prevent reduced VL and thigh flexor muscles and increased MuRF1 expression level during cast immobilization. Further study is required to confirm these results.https://doi.org/10.14814/phy2.14516MuRF1muscle atrophymuscle disuse |
| spellingShingle | Saori Kakehi Yoshifumi Tamura Atsushi Kubota Kageumi Takeno Minako Kawaguchi Keishoku Sakuraba Ryuzo Kawamori Hirotaka Watada Effects of blood flow restriction on muscle size and gene expression in muscle during immobilization: A pilot study Physiological Reports MuRF1 muscle atrophy muscle disuse |
| title | Effects of blood flow restriction on muscle size and gene expression in muscle during immobilization: A pilot study |
| title_full | Effects of blood flow restriction on muscle size and gene expression in muscle during immobilization: A pilot study |
| title_fullStr | Effects of blood flow restriction on muscle size and gene expression in muscle during immobilization: A pilot study |
| title_full_unstemmed | Effects of blood flow restriction on muscle size and gene expression in muscle during immobilization: A pilot study |
| title_short | Effects of blood flow restriction on muscle size and gene expression in muscle during immobilization: A pilot study |
| title_sort | effects of blood flow restriction on muscle size and gene expression in muscle during immobilization a pilot study |
| topic | MuRF1 muscle atrophy muscle disuse |
| url | https://doi.org/10.14814/phy2.14516 |
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