How does oestradiol influence the AVT/IT system in female round gobies during different reproductive phases?
In this in vitro gradient perfusion study, we determined whether there is a functional relationship between oestradiol and the arginine vasotocin/isotocin (AVT/IT) system in the female round goby (Neogobius melanostomus). Brain explants were perfused in medium supplemented with 17β-oestradiol (E2) a...
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| Format: | Article |
| Language: | English |
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The Company of Biologists
2017-10-01
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| Series: | Biology Open |
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| Online Access: | http://bio.biologists.org/content/6/10/1493 |
| _version_ | 1818922972736389120 |
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| author | Hanna Kalamarz-Kubiak Magdalena Gozdowska Tatiana Guellard Ewa Kulczykowska |
| author_facet | Hanna Kalamarz-Kubiak Magdalena Gozdowska Tatiana Guellard Ewa Kulczykowska |
| author_sort | Hanna Kalamarz-Kubiak |
| collection | DOAJ |
| description | In this in vitro gradient perfusion study, we determined whether there is a functional relationship between oestradiol and the arginine vasotocin/isotocin (AVT/IT) system in the female round goby (Neogobius melanostomus). Brain explants were perfused in medium supplemented with 17β-oestradiol (E2) at doses mimicking the plasma levels of this hormone in nature during the spawning-capable phase and regressing phase. We aimed to establish which pathway, genomic or non-genomic, is involved in this mechanism in different reproductive phases. For this purpose, brain explants were perfused in medium supplemented with Fulvestrant (ICI 182.780) or Actinomycin D (Act D) separately or in combination with E2. The contents of AVT and IT in the perfusion media were determined using high-performance liquid chromatography (HPLC) with fluorescence and UV detection. During the spawning-capable phase, the effect of E2 on AVT release is mediated through oestrogen receptors (ERs) via both genomic and non-genomic pathways, while IT release is mediated through ERs via a genomic pathway only. In the regressing phase, release of both nonapeptides is mediated through ERs via a genomic pathway. This is the first study to present a feasible mechanism of oestradiol action on the AVT/IT system in female fish during different phases of the reproductive cycle. |
| first_indexed | 2024-12-20T02:02:02Z |
| format | Article |
| id | doaj.art-ff20d140c4a349429b39d503a1809ae0 |
| institution | Directory Open Access Journal |
| issn | 2046-6390 |
| language | English |
| last_indexed | 2024-12-20T02:02:02Z |
| publishDate | 2017-10-01 |
| publisher | The Company of Biologists |
| record_format | Article |
| series | Biology Open |
| spelling | doaj.art-ff20d140c4a349429b39d503a1809ae02022-12-21T19:57:18ZengThe Company of BiologistsBiology Open2046-63902017-10-016101493150110.1242/bio.024844024844How does oestradiol influence the AVT/IT system in female round gobies during different reproductive phases?Hanna Kalamarz-Kubiak0Magdalena Gozdowska1Tatiana Guellard2Ewa Kulczykowska3 Department of Genetics and Marine Biotechnology, Institute of Oceanology Polish, Academy of Sciences, Powstańców Warszawy 55, 81-712 Sopot, Poland Department of Genetics and Marine Biotechnology, Institute of Oceanology Polish, Academy of Sciences, Powstańców Warszawy 55, 81-712 Sopot, Poland Department of Genetics and Marine Biotechnology, Institute of Oceanology Polish, Academy of Sciences, Powstańców Warszawy 55, 81-712 Sopot, Poland Department of Genetics and Marine Biotechnology, Institute of Oceanology Polish, Academy of Sciences, Powstańców Warszawy 55, 81-712 Sopot, Poland In this in vitro gradient perfusion study, we determined whether there is a functional relationship between oestradiol and the arginine vasotocin/isotocin (AVT/IT) system in the female round goby (Neogobius melanostomus). Brain explants were perfused in medium supplemented with 17β-oestradiol (E2) at doses mimicking the plasma levels of this hormone in nature during the spawning-capable phase and regressing phase. We aimed to establish which pathway, genomic or non-genomic, is involved in this mechanism in different reproductive phases. For this purpose, brain explants were perfused in medium supplemented with Fulvestrant (ICI 182.780) or Actinomycin D (Act D) separately or in combination with E2. The contents of AVT and IT in the perfusion media were determined using high-performance liquid chromatography (HPLC) with fluorescence and UV detection. During the spawning-capable phase, the effect of E2 on AVT release is mediated through oestrogen receptors (ERs) via both genomic and non-genomic pathways, while IT release is mediated through ERs via a genomic pathway only. In the regressing phase, release of both nonapeptides is mediated through ERs via a genomic pathway. This is the first study to present a feasible mechanism of oestradiol action on the AVT/IT system in female fish during different phases of the reproductive cycle.http://bio.biologists.org/content/6/10/1493OestradiolAVTITIn vitro gradient perfusionGenomic and non-genomic pathwaysFemale round goby |
| spellingShingle | Hanna Kalamarz-Kubiak Magdalena Gozdowska Tatiana Guellard Ewa Kulczykowska How does oestradiol influence the AVT/IT system in female round gobies during different reproductive phases? Biology Open Oestradiol AVT IT In vitro gradient perfusion Genomic and non-genomic pathways Female round goby |
| title | How does oestradiol influence the AVT/IT system in female round gobies during different reproductive phases? |
| title_full | How does oestradiol influence the AVT/IT system in female round gobies during different reproductive phases? |
| title_fullStr | How does oestradiol influence the AVT/IT system in female round gobies during different reproductive phases? |
| title_full_unstemmed | How does oestradiol influence the AVT/IT system in female round gobies during different reproductive phases? |
| title_short | How does oestradiol influence the AVT/IT system in female round gobies during different reproductive phases? |
| title_sort | how does oestradiol influence the avt it system in female round gobies during different reproductive phases |
| topic | Oestradiol AVT IT In vitro gradient perfusion Genomic and non-genomic pathways Female round goby |
| url | http://bio.biologists.org/content/6/10/1493 |
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