Karyotypes and <i>COI</i> Gene Sequences of <i>Chironomus</i> sp. Le1 (Kiknadze and Salova, 1996), <i>Ch. laetus</i> (Belyanina and Filinkova, 1996) and Their Hybrid from the Yamal Peninsula, Arctic Zone of Russia
The study of the biological diversity of the Arctic zone yields intriguing results. Initial research on the lakes of the Yamal Peninsula resulted in the identification of <i>Chironomus laetus</i> and the hybrid <i>Ch. laetus</i> × <i>Ch</i>. sp. Le1. To avoid misi...
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MDPI AG
2022-11-01
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| author | Viktor Bolshakov Alexander Prokin Dmitry Pavlov Azamat Akkizov Ekaterina Movergoz |
| author_facet | Viktor Bolshakov Alexander Prokin Dmitry Pavlov Azamat Akkizov Ekaterina Movergoz |
| author_sort | Viktor Bolshakov |
| collection | DOAJ |
| description | The study of the biological diversity of the Arctic zone yields intriguing results. Initial research on the lakes of the Yamal Peninsula resulted in the identification of <i>Chironomus laetus</i> and the hybrid <i>Ch. laetus</i> × <i>Ch</i>. sp. Le1. To avoid misidentification, we used morphological, cytogenetic, and molecular genetic approaches. By cytogenetics, in <i>Ch</i>. sp. Le1, seven banding sequences were found: Le1A1, Le1B1, Le1C1, Le1D1, Le1E1, Le1F1, and Le1G1. The karyotype of <i>Ch. laetus</i> was mapped for the first time; it is the first species with the arm combinations AE BC DF G. We propose the name of a new cytocomplex—“laetus”. DNA-barcoding of the <i>COI</i> gene was carried out for <i>Ch. laetus</i> and <i>Ch. laetus</i> × <i>Ch.</i> sp. Le1 for the first time. The estimated genetic distance between the sequences of <i>Ch. laetus</i> and <i>Ch. riihimakiensis</i> is 2.3–2.5%. The high similarity in morphology, banding sequences, and the possibility of hybridization indicate a close relationship between <i>Ch. laetus</i> and <i>Ch</i>. sp. Le1, which is assumed to be the northern variant of <i>Ch. riihimakiensis</i>. Molecular genetic data suggests the presence of a subgroup with <i>Ch. laetus</i>. |
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| spelling | doaj.art-ff23c010f99f4cae80c4fa529bfe72802023-11-24T15:39:21ZengMDPI AGInsects2075-44502022-11-011312111210.3390/insects13121112Karyotypes and <i>COI</i> Gene Sequences of <i>Chironomus</i> sp. Le1 (Kiknadze and Salova, 1996), <i>Ch. laetus</i> (Belyanina and Filinkova, 1996) and Their Hybrid from the Yamal Peninsula, Arctic Zone of RussiaViktor Bolshakov0Alexander Prokin1Dmitry Pavlov2Azamat Akkizov3Ekaterina Movergoz4Papanin Institute for Biology of Inland Waters Russian Academy of Sciences, Yaroslavl reg., Nekouz prov., Borok 152742, RussiaPapanin Institute for Biology of Inland Waters Russian Academy of Sciences, Yaroslavl reg., Nekouz prov., Borok 152742, RussiaPapanin Institute for Biology of Inland Waters Russian Academy of Sciences, Yaroslavl reg., Nekouz prov., Borok 152742, RussiaDepartment of Biology, Geoecology and Molecular Genetic Foundations of Living Systems, Kabardino-Balkarian State University Named after H. M. Berbekov, Chernyshevsky St., 173, Nalchik 360004, RussiaPapanin Institute for Biology of Inland Waters Russian Academy of Sciences, Yaroslavl reg., Nekouz prov., Borok 152742, RussiaThe study of the biological diversity of the Arctic zone yields intriguing results. Initial research on the lakes of the Yamal Peninsula resulted in the identification of <i>Chironomus laetus</i> and the hybrid <i>Ch. laetus</i> × <i>Ch</i>. sp. Le1. To avoid misidentification, we used morphological, cytogenetic, and molecular genetic approaches. By cytogenetics, in <i>Ch</i>. sp. Le1, seven banding sequences were found: Le1A1, Le1B1, Le1C1, Le1D1, Le1E1, Le1F1, and Le1G1. The karyotype of <i>Ch. laetus</i> was mapped for the first time; it is the first species with the arm combinations AE BC DF G. We propose the name of a new cytocomplex—“laetus”. DNA-barcoding of the <i>COI</i> gene was carried out for <i>Ch. laetus</i> and <i>Ch. laetus</i> × <i>Ch.</i> sp. Le1 for the first time. The estimated genetic distance between the sequences of <i>Ch. laetus</i> and <i>Ch. riihimakiensis</i> is 2.3–2.5%. The high similarity in morphology, banding sequences, and the possibility of hybridization indicate a close relationship between <i>Ch. laetus</i> and <i>Ch</i>. sp. Le1, which is assumed to be the northern variant of <i>Ch. riihimakiensis</i>. Molecular genetic data suggests the presence of a subgroup with <i>Ch. laetus</i>.https://www.mdpi.com/2075-4450/13/12/1112karyotypehybridChironomus<i>Chironomus laetus</i><i>Ch.</i> sp. Le1COI |
| spellingShingle | Viktor Bolshakov Alexander Prokin Dmitry Pavlov Azamat Akkizov Ekaterina Movergoz Karyotypes and <i>COI</i> Gene Sequences of <i>Chironomus</i> sp. Le1 (Kiknadze and Salova, 1996), <i>Ch. laetus</i> (Belyanina and Filinkova, 1996) and Their Hybrid from the Yamal Peninsula, Arctic Zone of Russia Insects karyotype hybrid Chironomus <i>Chironomus laetus</i> <i>Ch.</i> sp. Le1 COI |
| title | Karyotypes and <i>COI</i> Gene Sequences of <i>Chironomus</i> sp. Le1 (Kiknadze and Salova, 1996), <i>Ch. laetus</i> (Belyanina and Filinkova, 1996) and Their Hybrid from the Yamal Peninsula, Arctic Zone of Russia |
| title_full | Karyotypes and <i>COI</i> Gene Sequences of <i>Chironomus</i> sp. Le1 (Kiknadze and Salova, 1996), <i>Ch. laetus</i> (Belyanina and Filinkova, 1996) and Their Hybrid from the Yamal Peninsula, Arctic Zone of Russia |
| title_fullStr | Karyotypes and <i>COI</i> Gene Sequences of <i>Chironomus</i> sp. Le1 (Kiknadze and Salova, 1996), <i>Ch. laetus</i> (Belyanina and Filinkova, 1996) and Their Hybrid from the Yamal Peninsula, Arctic Zone of Russia |
| title_full_unstemmed | Karyotypes and <i>COI</i> Gene Sequences of <i>Chironomus</i> sp. Le1 (Kiknadze and Salova, 1996), <i>Ch. laetus</i> (Belyanina and Filinkova, 1996) and Their Hybrid from the Yamal Peninsula, Arctic Zone of Russia |
| title_short | Karyotypes and <i>COI</i> Gene Sequences of <i>Chironomus</i> sp. Le1 (Kiknadze and Salova, 1996), <i>Ch. laetus</i> (Belyanina and Filinkova, 1996) and Their Hybrid from the Yamal Peninsula, Arctic Zone of Russia |
| title_sort | karyotypes and i coi i gene sequences of i chironomus i sp le1 kiknadze and salova 1996 i ch laetus i belyanina and filinkova 1996 and their hybrid from the yamal peninsula arctic zone of russia |
| topic | karyotype hybrid Chironomus <i>Chironomus laetus</i> <i>Ch.</i> sp. Le1 COI |
| url | https://www.mdpi.com/2075-4450/13/12/1112 |
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