Preliminary evaluation on the efficiency of the kit Platelia Dengue NS1 Ag-ELISA to detect dengue virus in dried Aedes aegypti: a potential tool to improve dengue surveillance
Abstract Background Surveillance is a critical component of any dengue prevention and control programme. Herein, we investigate the efficiency of the commercial kit Platelia Dengue NS1 Ag-ELISA to detect dengue virus (DENV) antigens in Aedes aegypti mosquitoes infected under laboratory conditions. M...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2014-04-01
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| Series: | Parasites & Vectors |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/1756-3305-7-155 |
| _version_ | 1797811726658306048 |
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| author | Gabriel Sylvestre Mariana Gandini Josélio MG de Araújo Claire F Kubelka Ricardo Lourenço-de-Oliveira Rafael Maciel-de-Freitas |
| author_facet | Gabriel Sylvestre Mariana Gandini Josélio MG de Araújo Claire F Kubelka Ricardo Lourenço-de-Oliveira Rafael Maciel-de-Freitas |
| author_sort | Gabriel Sylvestre |
| collection | DOAJ |
| description | Abstract Background Surveillance is a critical component of any dengue prevention and control programme. Herein, we investigate the efficiency of the commercial kit Platelia Dengue NS1 Ag-ELISA to detect dengue virus (DENV) antigens in Aedes aegypti mosquitoes infected under laboratory conditions. Methods Under insectary conditions, four to five day-old mosquitoes were orally challenged with DENV-2 titer of 3.6 x 105 PFU equivalent/ml, incubated for 14 days and then killed. At ten time-points following mosquito death (0, 6, 12, 24, 72, 96, 120, 144 and 168 h), i.e., during a one-week period, dried mosquitoes were comparatively tested for the detection of the NS1 antigen with other methods of detection, such as qRT-PCR and virus isolation in C6/36 cells. Results We first observed that the NS1 antigen was more effective in detecting DENV-2 in Ae. aegypti between 12 and 72 h after mosquito death when compared with qRT-PCR. A second round involved comparing the sensitivity of detection of the NS1 antigen and virus isolation in C6/36 cells. The NS1 antigen was also more effective than virus isolation, detecting DENV-2 at all time-points, i.e., up to 168 h after mosquito death. Meanwhile, virus isolation was successful up to 96 h after Ae. aegypti death, but the number of positive samples per time period presented a tendency to decline progressively over time. From the 43 samples positive by the virus isolation technique, 38 (88.4%) were also positive by the NS1 test. Conclusion Taken together, these results are the first to indicate that the NS1 antigen might be an interesting complementary tool to improve dengue surveillance through DENV detection in dried Ae. aegypti females. |
| first_indexed | 2024-03-13T07:27:59Z |
| format | Article |
| id | doaj.art-ff23d579fc394bef989239a5998901c3 |
| institution | Directory Open Access Journal |
| issn | 1756-3305 |
| language | English |
| last_indexed | 2024-03-13T07:27:59Z |
| publishDate | 2014-04-01 |
| publisher | BMC |
| record_format | Article |
| series | Parasites & Vectors |
| spelling | doaj.art-ff23d579fc394bef989239a5998901c32023-06-04T11:19:28ZengBMCParasites & Vectors1756-33052014-04-01711710.1186/1756-3305-7-155Preliminary evaluation on the efficiency of the kit Platelia Dengue NS1 Ag-ELISA to detect dengue virus in dried Aedes aegypti: a potential tool to improve dengue surveillanceGabriel Sylvestre0Mariana Gandini1Josélio MG de Araújo2Claire F Kubelka3Ricardo Lourenço-de-Oliveira4Rafael Maciel-de-Freitas5Laboratório de Transmissores de Hematozoários, Instituto Oswaldo Cruz, FiocruzLaboratório de Imunologia Viral, Instituto Oswaldo Cruz, FiocruzLaboratório de Biologia Molecular de Doenças Infecciosas e do Câncer, Departamento de Microbiologia e Parasitologia, Universidade Federal do Rio Grande do NorteLaboratório de Imunologia Viral, Instituto Oswaldo Cruz, FiocruzLaboratório de Transmissores de Hematozoários, Instituto Oswaldo Cruz, FiocruzLaboratório de Transmissores de Hematozoários, Instituto Oswaldo Cruz, FiocruzAbstract Background Surveillance is a critical component of any dengue prevention and control programme. Herein, we investigate the efficiency of the commercial kit Platelia Dengue NS1 Ag-ELISA to detect dengue virus (DENV) antigens in Aedes aegypti mosquitoes infected under laboratory conditions. Methods Under insectary conditions, four to five day-old mosquitoes were orally challenged with DENV-2 titer of 3.6 x 105 PFU equivalent/ml, incubated for 14 days and then killed. At ten time-points following mosquito death (0, 6, 12, 24, 72, 96, 120, 144 and 168 h), i.e., during a one-week period, dried mosquitoes were comparatively tested for the detection of the NS1 antigen with other methods of detection, such as qRT-PCR and virus isolation in C6/36 cells. Results We first observed that the NS1 antigen was more effective in detecting DENV-2 in Ae. aegypti between 12 and 72 h after mosquito death when compared with qRT-PCR. A second round involved comparing the sensitivity of detection of the NS1 antigen and virus isolation in C6/36 cells. The NS1 antigen was also more effective than virus isolation, detecting DENV-2 at all time-points, i.e., up to 168 h after mosquito death. Meanwhile, virus isolation was successful up to 96 h after Ae. aegypti death, but the number of positive samples per time period presented a tendency to decline progressively over time. From the 43 samples positive by the virus isolation technique, 38 (88.4%) were also positive by the NS1 test. Conclusion Taken together, these results are the first to indicate that the NS1 antigen might be an interesting complementary tool to improve dengue surveillance through DENV detection in dried Ae. aegypti females.https://doi.org/10.1186/1756-3305-7-155Aedes aegyptiDengueSurveillanceNS1ELISARNA |
| spellingShingle | Gabriel Sylvestre Mariana Gandini Josélio MG de Araújo Claire F Kubelka Ricardo Lourenço-de-Oliveira Rafael Maciel-de-Freitas Preliminary evaluation on the efficiency of the kit Platelia Dengue NS1 Ag-ELISA to detect dengue virus in dried Aedes aegypti: a potential tool to improve dengue surveillance Parasites & Vectors Aedes aegypti Dengue Surveillance NS1 ELISA RNA |
| title | Preliminary evaluation on the efficiency of the kit Platelia Dengue NS1 Ag-ELISA to detect dengue virus in dried Aedes aegypti: a potential tool to improve dengue surveillance |
| title_full | Preliminary evaluation on the efficiency of the kit Platelia Dengue NS1 Ag-ELISA to detect dengue virus in dried Aedes aegypti: a potential tool to improve dengue surveillance |
| title_fullStr | Preliminary evaluation on the efficiency of the kit Platelia Dengue NS1 Ag-ELISA to detect dengue virus in dried Aedes aegypti: a potential tool to improve dengue surveillance |
| title_full_unstemmed | Preliminary evaluation on the efficiency of the kit Platelia Dengue NS1 Ag-ELISA to detect dengue virus in dried Aedes aegypti: a potential tool to improve dengue surveillance |
| title_short | Preliminary evaluation on the efficiency of the kit Platelia Dengue NS1 Ag-ELISA to detect dengue virus in dried Aedes aegypti: a potential tool to improve dengue surveillance |
| title_sort | preliminary evaluation on the efficiency of the kit platelia dengue ns1 ag elisa to detect dengue virus in dried aedes aegypti a potential tool to improve dengue surveillance |
| topic | Aedes aegypti Dengue Surveillance NS1 ELISA RNA |
| url | https://doi.org/10.1186/1756-3305-7-155 |
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