Differentiation of Phenolic Composition Among Tunisian <i>Thymus algeriensis</i> Boiss. et Reut. (<i>Lamiaceae</i>) Populations: Correlation to Bioactive Activities
Twelve Tunisian <i>Thymus algeriensis</i> populations growing wild in different bioclimatic zones, extending from the subhumid to the upper-arid bioclimates, were compared regarding their phenolic composition and their ability to serve as antioxidant, anti-acetylcholinesterase, and antib...
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| Format: | Article |
| Language: | English |
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MDPI AG
2019-10-01
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| Series: | Antioxidants |
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| Online Access: | https://www.mdpi.com/2076-3921/8/11/515 |
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| author | Rym Jaouadi Artur M. S. Silva Mohamed Boussaid Imen B. H. Yahia Susana M. Cardoso Yosr Zaouali |
| author_facet | Rym Jaouadi Artur M. S. Silva Mohamed Boussaid Imen B. H. Yahia Susana M. Cardoso Yosr Zaouali |
| author_sort | Rym Jaouadi |
| collection | DOAJ |
| description | Twelve Tunisian <i>Thymus algeriensis</i> populations growing wild in different bioclimatic zones, extending from the subhumid to the upper-arid bioclimates, were compared regarding their phenolic composition and their ability to serve as antioxidant, anti-acetylcholinesterase, and antibacterial agents. A significant variation of phenol profile was observed between the analyzed populations, as assessed by ultra-high-performance liquid chromatography coupled with a diode array detector and an electrospray mass spectrometer (UHPLC-DAD-ESI/MS<sup>n</sup>) technique. Rosmarinic acid was the main phenolic compound in most populations (383.8−1157.8 µg/mL extract), but still, those from the upper-arid bioclimatic zone were distinguished by the presence of carvacrol (1374.7 and 2221.6 µg/mL extract), which was absent in the remaining ones. <i>T. algeriensis</i> methanolic extracts were found to possess a substantial antioxidant and anti-acetylcholinesterase activities, with significant variation observed between populations, which were correlated to their phenolic contents. The antibacterial activity of the extracts tested against seven bacteria was revealed only by populations collected from upper-arid bioclimate and mainly associated with the presence of carvacrol. Extracts revealed a bacteriostatic effect against all bacteria (MIC = 1.4 mg/mL). Yet, the bactericidal activity (MBC = 1.4mg/mL) was restricted to the gram-negative bacteria <i>Escherchia coli.</i> |
| first_indexed | 2024-03-12T11:16:15Z |
| format | Article |
| id | doaj.art-ff248fb658284330b605931b034403cf |
| institution | Directory Open Access Journal |
| issn | 2076-3921 |
| language | English |
| last_indexed | 2024-03-12T11:16:15Z |
| publishDate | 2019-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Antioxidants |
| spelling | doaj.art-ff248fb658284330b605931b034403cf2023-09-02T02:04:30ZengMDPI AGAntioxidants2076-39212019-10-0181151510.3390/antiox8110515antiox8110515Differentiation of Phenolic Composition Among Tunisian <i>Thymus algeriensis</i> Boiss. et Reut. (<i>Lamiaceae</i>) Populations: Correlation to Bioactive ActivitiesRym Jaouadi0Artur M. S. Silva1Mohamed Boussaid2Imen B. H. Yahia3Susana M. Cardoso4Yosr Zaouali5Laboratory of Plant Biotechnology, Department of Biology, National Institute of Applied Science and Technology, B.P. 676, Tunis CEDEX 1080, TunisiaQOPNA & LAQV-REQUIMTE, Department of Chemistry, University of Aveiro, 3810-193 Aveiro, PortugalLaboratory of Plant Biotechnology, Department of Biology, National Institute of Applied Science and Technology, B.P. 676, Tunis CEDEX 1080, TunisiaLaboratory of Plant Biotechnology, Department of Biology, National Institute of Applied Science and Technology, B.P. 676, Tunis CEDEX 1080, TunisiaQOPNA & LAQV-REQUIMTE, Department of Chemistry, University of Aveiro, 3810-193 Aveiro, PortugalLaboratory of Plant Biotechnology, Department of Biology, National Institute of Applied Science and Technology, B.P. 676, Tunis CEDEX 1080, TunisiaTwelve Tunisian <i>Thymus algeriensis</i> populations growing wild in different bioclimatic zones, extending from the subhumid to the upper-arid bioclimates, were compared regarding their phenolic composition and their ability to serve as antioxidant, anti-acetylcholinesterase, and antibacterial agents. A significant variation of phenol profile was observed between the analyzed populations, as assessed by ultra-high-performance liquid chromatography coupled with a diode array detector and an electrospray mass spectrometer (UHPLC-DAD-ESI/MS<sup>n</sup>) technique. Rosmarinic acid was the main phenolic compound in most populations (383.8−1157.8 µg/mL extract), but still, those from the upper-arid bioclimatic zone were distinguished by the presence of carvacrol (1374.7 and 2221.6 µg/mL extract), which was absent in the remaining ones. <i>T. algeriensis</i> methanolic extracts were found to possess a substantial antioxidant and anti-acetylcholinesterase activities, with significant variation observed between populations, which were correlated to their phenolic contents. The antibacterial activity of the extracts tested against seven bacteria was revealed only by populations collected from upper-arid bioclimate and mainly associated with the presence of carvacrol. Extracts revealed a bacteriostatic effect against all bacteria (MIC = 1.4 mg/mL). Yet, the bactericidal activity (MBC = 1.4mg/mL) was restricted to the gram-negative bacteria <i>Escherchia coli.</i>https://www.mdpi.com/2076-3921/8/11/515thymeuhplc-dad-esi/ms<sup>n</sup>phenolic compoundsantioxidant activityanti-acetylcholinesterase activityantibacterial activity |
| spellingShingle | Rym Jaouadi Artur M. S. Silva Mohamed Boussaid Imen B. H. Yahia Susana M. Cardoso Yosr Zaouali Differentiation of Phenolic Composition Among Tunisian <i>Thymus algeriensis</i> Boiss. et Reut. (<i>Lamiaceae</i>) Populations: Correlation to Bioactive Activities Antioxidants thyme uhplc-dad-esi/ms<sup>n</sup> phenolic compounds antioxidant activity anti-acetylcholinesterase activity antibacterial activity |
| title | Differentiation of Phenolic Composition Among Tunisian <i>Thymus algeriensis</i> Boiss. et Reut. (<i>Lamiaceae</i>) Populations: Correlation to Bioactive Activities |
| title_full | Differentiation of Phenolic Composition Among Tunisian <i>Thymus algeriensis</i> Boiss. et Reut. (<i>Lamiaceae</i>) Populations: Correlation to Bioactive Activities |
| title_fullStr | Differentiation of Phenolic Composition Among Tunisian <i>Thymus algeriensis</i> Boiss. et Reut. (<i>Lamiaceae</i>) Populations: Correlation to Bioactive Activities |
| title_full_unstemmed | Differentiation of Phenolic Composition Among Tunisian <i>Thymus algeriensis</i> Boiss. et Reut. (<i>Lamiaceae</i>) Populations: Correlation to Bioactive Activities |
| title_short | Differentiation of Phenolic Composition Among Tunisian <i>Thymus algeriensis</i> Boiss. et Reut. (<i>Lamiaceae</i>) Populations: Correlation to Bioactive Activities |
| title_sort | differentiation of phenolic composition among tunisian i thymus algeriensis i boiss et reut i lamiaceae i populations correlation to bioactive activities |
| topic | thyme uhplc-dad-esi/ms<sup>n</sup> phenolic compounds antioxidant activity anti-acetylcholinesterase activity antibacterial activity |
| url | https://www.mdpi.com/2076-3921/8/11/515 |
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