Transcriptomic analysis of LMH cells in response to the overexpression of a protein of Eimeria tenella encoded by the locus ETH_00028350
A protein of Eimeria tenella (encoded by the locus ETH_00028350) homologous to Toxoplasma gondii dense granule protein 9, designated as EtHGRA9 hereafter, was reported to be expressed in all life cycle stages of E. tenella. However, no data are currently available regarding its functional properties...
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Frontiers Media S.A.
2022-11-01
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| Series: | Frontiers in Veterinary Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fvets.2022.1053701/full |
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| author | Xiao-Jing Wu Jin Gao Bing-Jin Mu Lin-Mei Yu Zi-Rui Wang Wen-Bin Zheng Wen-Wei Gao Xing-Quan Zhu Xing-Quan Zhu Qing Liu |
| author_facet | Xiao-Jing Wu Jin Gao Bing-Jin Mu Lin-Mei Yu Zi-Rui Wang Wen-Bin Zheng Wen-Wei Gao Xing-Quan Zhu Xing-Quan Zhu Qing Liu |
| author_sort | Xiao-Jing Wu |
| collection | DOAJ |
| description | A protein of Eimeria tenella (encoded by the locus ETH_00028350) homologous to Toxoplasma gondii dense granule protein 9, designated as EtHGRA9 hereafter, was reported to be expressed in all life cycle stages of E. tenella. However, no data are currently available regarding its functional properties. In the present study, a recombinant vector harboring a 741 bp gene segment encoding the mature form of EtHGRA9 was constructed and transfected into leghorn male hepatoma (LMH) cells. Then, transcriptomic analysis of the transfected LMH cells was carried out by using a high-throughput RNA-seq technology. The LMH cells overexpressing EtHGRA9 was validated by means of Western blotting as well as indirect immunofluorescence staining. The results demonstrated that the expression of 547 genes (275 upregulated genes and 272 downregulated genes) was altered by EtHGRA9. The quantitative real-time polymerase chain reaction (qRT-PCR) validation of the ten genes with differential expression between the two groups was consistent with the transcriptome analysis. According to pathway enrichment analysis for the obtained differentially expressed genes, seven pathways were significantly affected by EtHGRA9, such as cytokine-cytokine receptor interaction, MAPK signaling pathway, and protein processing in endoplasmic reticulum. Our data reveal several possible roles of EtHGRA9 in immune or inflammatory responses, which paves the way for a better understanding of the molecular interplay between E. tenella and its host. |
| first_indexed | 2024-04-12T07:27:41Z |
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| id | doaj.art-ff25cc48bfe7435b91a67fa8f8b1eb62 |
| institution | Directory Open Access Journal |
| issn | 2297-1769 |
| language | English |
| last_indexed | 2024-04-12T07:27:41Z |
| publishDate | 2022-11-01 |
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| series | Frontiers in Veterinary Science |
| spelling | doaj.art-ff25cc48bfe7435b91a67fa8f8b1eb622022-12-22T03:42:09ZengFrontiers Media S.A.Frontiers in Veterinary Science2297-17692022-11-01910.3389/fvets.2022.10537011053701Transcriptomic analysis of LMH cells in response to the overexpression of a protein of Eimeria tenella encoded by the locus ETH_00028350Xiao-Jing Wu0Jin Gao1Bing-Jin Mu2Lin-Mei Yu3Zi-Rui Wang4Wen-Bin Zheng5Wen-Wei Gao6Xing-Quan Zhu7Xing-Quan Zhu8Qing Liu9Laboratory of Parasitic Diseases, College of Veterinary Medicine, Shanxi Agricultural University, Taigu, Shanxi, ChinaLaboratory of Parasitic Diseases, College of Veterinary Medicine, Shanxi Agricultural University, Taigu, Shanxi, ChinaLaboratory of Parasitic Diseases, College of Veterinary Medicine, Shanxi Agricultural University, Taigu, Shanxi, ChinaLaboratory of Parasitic Diseases, College of Veterinary Medicine, Shanxi Agricultural University, Taigu, Shanxi, ChinaLaboratory of Parasitic Diseases, College of Veterinary Medicine, Shanxi Agricultural University, Taigu, Shanxi, ChinaLaboratory of Parasitic Diseases, College of Veterinary Medicine, Shanxi Agricultural University, Taigu, Shanxi, ChinaLaboratory of Parasitic Diseases, College of Veterinary Medicine, Shanxi Agricultural University, Taigu, Shanxi, ChinaLaboratory of Parasitic Diseases, College of Veterinary Medicine, Shanxi Agricultural University, Taigu, Shanxi, ChinaKey Laboratory of Veterinary Public Health of Higher Education of Yunnan Province, College of Veterinary Medicine, Yunnan Agricultural University, Kunming, Yunnan, ChinaLaboratory of Parasitic Diseases, College of Veterinary Medicine, Shanxi Agricultural University, Taigu, Shanxi, ChinaA protein of Eimeria tenella (encoded by the locus ETH_00028350) homologous to Toxoplasma gondii dense granule protein 9, designated as EtHGRA9 hereafter, was reported to be expressed in all life cycle stages of E. tenella. However, no data are currently available regarding its functional properties. In the present study, a recombinant vector harboring a 741 bp gene segment encoding the mature form of EtHGRA9 was constructed and transfected into leghorn male hepatoma (LMH) cells. Then, transcriptomic analysis of the transfected LMH cells was carried out by using a high-throughput RNA-seq technology. The LMH cells overexpressing EtHGRA9 was validated by means of Western blotting as well as indirect immunofluorescence staining. The results demonstrated that the expression of 547 genes (275 upregulated genes and 272 downregulated genes) was altered by EtHGRA9. The quantitative real-time polymerase chain reaction (qRT-PCR) validation of the ten genes with differential expression between the two groups was consistent with the transcriptome analysis. According to pathway enrichment analysis for the obtained differentially expressed genes, seven pathways were significantly affected by EtHGRA9, such as cytokine-cytokine receptor interaction, MAPK signaling pathway, and protein processing in endoplasmic reticulum. Our data reveal several possible roles of EtHGRA9 in immune or inflammatory responses, which paves the way for a better understanding of the molecular interplay between E. tenella and its host.https://www.frontiersin.org/articles/10.3389/fvets.2022.1053701/fullEimeria tenellaLMH cellsdense granule protein 9overexpressiontranscriptome |
| spellingShingle | Xiao-Jing Wu Jin Gao Bing-Jin Mu Lin-Mei Yu Zi-Rui Wang Wen-Bin Zheng Wen-Wei Gao Xing-Quan Zhu Xing-Quan Zhu Qing Liu Transcriptomic analysis of LMH cells in response to the overexpression of a protein of Eimeria tenella encoded by the locus ETH_00028350 Frontiers in Veterinary Science Eimeria tenella LMH cells dense granule protein 9 overexpression transcriptome |
| title | Transcriptomic analysis of LMH cells in response to the overexpression of a protein of Eimeria tenella encoded by the locus ETH_00028350 |
| title_full | Transcriptomic analysis of LMH cells in response to the overexpression of a protein of Eimeria tenella encoded by the locus ETH_00028350 |
| title_fullStr | Transcriptomic analysis of LMH cells in response to the overexpression of a protein of Eimeria tenella encoded by the locus ETH_00028350 |
| title_full_unstemmed | Transcriptomic analysis of LMH cells in response to the overexpression of a protein of Eimeria tenella encoded by the locus ETH_00028350 |
| title_short | Transcriptomic analysis of LMH cells in response to the overexpression of a protein of Eimeria tenella encoded by the locus ETH_00028350 |
| title_sort | transcriptomic analysis of lmh cells in response to the overexpression of a protein of eimeria tenella encoded by the locus eth 00028350 |
| topic | Eimeria tenella LMH cells dense granule protein 9 overexpression transcriptome |
| url | https://www.frontiersin.org/articles/10.3389/fvets.2022.1053701/full |
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