Differential Expression and Localization of EHBP1L1 during the First Wave of Rat Spermatogenesis Suggest Its Involvement in Acrosome Biogenesis
The identification and characterization of new proteins involved in spermatogenesis is fundamental, considering that good-quality gametes are basic in ensuring proper reproduction. Here, we further analyzed the temporal and spatial localization during the first spermatogenic wave of rat testis of EH...
| Những tác giả chính: | , |
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| Định dạng: | Bài viết |
| Ngôn ngữ: | English |
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MDPI AG
2022-01-01
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| Loạt: | Biomedicines |
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| Truy cập trực tuyến: | https://www.mdpi.com/2227-9059/10/1/181 |
| _version_ | 1827666430285316096 |
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| author | Massimo Venditti Sergio Minucci |
| author_facet | Massimo Venditti Sergio Minucci |
| author_sort | Massimo Venditti |
| collection | DOAJ |
| description | The identification and characterization of new proteins involved in spermatogenesis is fundamental, considering that good-quality gametes are basic in ensuring proper reproduction. Here, we further analyzed the temporal and spatial localization during the first spermatogenic wave of rat testis of EHBP1L1, which is involved in vesicular trafficking due to the CH and bMERB domains, which bind to actin and Rab8/10, respectively. Western blot and immunofluorescence analyses showed that EHBP1L1 protein expression started at 21 days post-partum (dpp) concomitantly with the appearance of primary spermatocytes (I SPC). In subsequent stages, EHBP1L1 specifically localized together with actin in the perinuclear cytoplasm close to the acrosomal and Golgian regions of spermatids (SPT) during the different phases of acrosome biogenesis (AB). Moreover, it was completely absent in elongated SPT and in mature spermatozoa, suggesting that its role was completed in previous stages. The combined data, also supported by our previous report demonstrating that <i>EHBP1L1</i> mRNA was expressed by primary (I) and secondary (II) SPC, lead us to hypothesize its specific role during AB. Although these results are suggestive, further studies are needed to better clarify the underlying molecular mechanisms of AB, with the aim to use EHBP1L1 as a potential new marker for spermatogenesis. |
| first_indexed | 2024-03-10T01:51:34Z |
| format | Article |
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| institution | Directory Open Access Journal |
| issn | 2227-9059 |
| language | English |
| last_indexed | 2024-03-10T01:51:34Z |
| publishDate | 2022-01-01 |
| publisher | MDPI AG |
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| series | Biomedicines |
| spelling | doaj.art-ff26936fc36e4b238c792caccafc00ca2023-11-23T13:05:10ZengMDPI AGBiomedicines2227-90592022-01-0110118110.3390/biomedicines10010181Differential Expression and Localization of EHBP1L1 during the First Wave of Rat Spermatogenesis Suggest Its Involvement in Acrosome BiogenesisMassimo Venditti0Sergio Minucci1Dipartimento di Medicina Sperimentale, Università degli Studi della Campania “Luigi Vanvitelli”, Via Costantinopoli 16, 80138 Napoli, ItalyDipartimento di Medicina Sperimentale, Università degli Studi della Campania “Luigi Vanvitelli”, Via Costantinopoli 16, 80138 Napoli, ItalyThe identification and characterization of new proteins involved in spermatogenesis is fundamental, considering that good-quality gametes are basic in ensuring proper reproduction. Here, we further analyzed the temporal and spatial localization during the first spermatogenic wave of rat testis of EHBP1L1, which is involved in vesicular trafficking due to the CH and bMERB domains, which bind to actin and Rab8/10, respectively. Western blot and immunofluorescence analyses showed that EHBP1L1 protein expression started at 21 days post-partum (dpp) concomitantly with the appearance of primary spermatocytes (I SPC). In subsequent stages, EHBP1L1 specifically localized together with actin in the perinuclear cytoplasm close to the acrosomal and Golgian regions of spermatids (SPT) during the different phases of acrosome biogenesis (AB). Moreover, it was completely absent in elongated SPT and in mature spermatozoa, suggesting that its role was completed in previous stages. The combined data, also supported by our previous report demonstrating that <i>EHBP1L1</i> mRNA was expressed by primary (I) and secondary (II) SPC, lead us to hypothesize its specific role during AB. Although these results are suggestive, further studies are needed to better clarify the underlying molecular mechanisms of AB, with the aim to use EHBP1L1 as a potential new marker for spermatogenesis.https://www.mdpi.com/2227-9059/10/1/181EHBP1L1acrosomerat testisspermatogenesisGolgi apparatuscytoskeleton |
| spellingShingle | Massimo Venditti Sergio Minucci Differential Expression and Localization of EHBP1L1 during the First Wave of Rat Spermatogenesis Suggest Its Involvement in Acrosome Biogenesis Biomedicines EHBP1L1 acrosome rat testis spermatogenesis Golgi apparatus cytoskeleton |
| title | Differential Expression and Localization of EHBP1L1 during the First Wave of Rat Spermatogenesis Suggest Its Involvement in Acrosome Biogenesis |
| title_full | Differential Expression and Localization of EHBP1L1 during the First Wave of Rat Spermatogenesis Suggest Its Involvement in Acrosome Biogenesis |
| title_fullStr | Differential Expression and Localization of EHBP1L1 during the First Wave of Rat Spermatogenesis Suggest Its Involvement in Acrosome Biogenesis |
| title_full_unstemmed | Differential Expression and Localization of EHBP1L1 during the First Wave of Rat Spermatogenesis Suggest Its Involvement in Acrosome Biogenesis |
| title_short | Differential Expression and Localization of EHBP1L1 during the First Wave of Rat Spermatogenesis Suggest Its Involvement in Acrosome Biogenesis |
| title_sort | differential expression and localization of ehbp1l1 during the first wave of rat spermatogenesis suggest its involvement in acrosome biogenesis |
| topic | EHBP1L1 acrosome rat testis spermatogenesis Golgi apparatus cytoskeleton |
| url | https://www.mdpi.com/2227-9059/10/1/181 |
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