The curious case of genome packaging and assembly in RNA viruses infecting plants
Genome packaging is the crucial step for maturation of plant viruses containing an RNA genome. Viruses exhibit a remarkable degree of packaging specificity, despite the probability of co-packaging cellular RNAs. Three different types of viral genome packaging systems are reported so far. The recentl...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2023-06-01
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| Series: | Frontiers in Genetics |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fgene.2023.1198647/full |
| _version_ | 1797809131678072832 |
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| author | Tushar Ranjan Ravi Ranjan Kumar Mohammad Ansar Jitesh Kumar Auroshikha Mohanty Anamika Kumari Khushbu Jain Kumari Rajani Sailabala Dei Mohammad Feza Ahmad |
| author_facet | Tushar Ranjan Ravi Ranjan Kumar Mohammad Ansar Jitesh Kumar Auroshikha Mohanty Anamika Kumari Khushbu Jain Kumari Rajani Sailabala Dei Mohammad Feza Ahmad |
| author_sort | Tushar Ranjan |
| collection | DOAJ |
| description | Genome packaging is the crucial step for maturation of plant viruses containing an RNA genome. Viruses exhibit a remarkable degree of packaging specificity, despite the probability of co-packaging cellular RNAs. Three different types of viral genome packaging systems are reported so far. The recently upgraded type I genome packaging system involves nucleation and encapsidation of RNA genomes in an energy-dependent manner, which have been observed in most of the plant RNA viruses with a smaller genome size, while type II and III packaging systems, majorly discovered in bacteriophages and large eukaryotic DNA viruses, involve genome translocation and packaging inside the prohead in an energy-dependent manner, i.e., utilizing ATP. Although ATP is essential for all three packaging systems, each machinery system employs a unique mode of ATP hydrolysis and genome packaging mechanism. Plant RNA viruses are serious threats to agricultural and horticultural crops and account for huge economic losses. Developing control strategies against plant RNA viruses requires a deep understanding of their genome assembly and packaging mechanism. On the basis of our previous studies and meticulously planned experiments, we have revealed their molecular mechanisms and proposed a hypothetical model for the type I packaging system with an emphasis on smaller plant RNA viruses. Here, in this review, we apprise researchers the technical breakthroughs that have facilitated the dissection of genome packaging and virion assembly processes in plant RNA viruses. |
| first_indexed | 2024-03-13T06:47:59Z |
| format | Article |
| id | doaj.art-ff28630c64e84da195481ade5218f045 |
| institution | Directory Open Access Journal |
| issn | 1664-8021 |
| language | English |
| last_indexed | 2024-03-13T06:47:59Z |
| publishDate | 2023-06-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Genetics |
| spelling | doaj.art-ff28630c64e84da195481ade5218f0452023-06-08T04:34:34ZengFrontiers Media S.A.Frontiers in Genetics1664-80212023-06-011410.3389/fgene.2023.11986471198647The curious case of genome packaging and assembly in RNA viruses infecting plantsTushar Ranjan0Ravi Ranjan Kumar1Mohammad Ansar2Jitesh Kumar3Auroshikha Mohanty4Anamika Kumari5Khushbu Jain6Kumari Rajani7Sailabala Dei8Mohammad Feza Ahmad9Department of Molecular Biology and Genetic Engineering, Bihar Agricultural University, Bhagalpur, Bihar, IndiaDepartment of Molecular Biology and Genetic Engineering, Bihar Agricultural University, Bhagalpur, Bihar, IndiaDepartment of Plant Pathology, Bihar Agricultural University, Bhagalpur, Bihar, IndiaDepartment of Molecular Biology and Genetic Engineering, Bihar Agricultural University, Bhagalpur, Bihar, IndiaDepartment of Molecular Biology and Genetic Engineering, Bihar Agricultural University, Bhagalpur, Bihar, IndiaDepartment of Molecular Biology and Genetic Engineering, Bihar Agricultural University, Bhagalpur, Bihar, IndiaDepartment of Molecular Biology and Genetic Engineering, Bihar Agricultural University, Bhagalpur, Bihar, IndiaDepartment of Seed Science and Technology, Bihar Agricultural University, Bhagalpur, Bihar, IndiaDeputy Director Research, Bihar Agricultural University, Bhagalpur, Bihar, IndiaDepartment of Horticulture, Bihar Agricultural University, Bhagalpur, Bihar, IndiaGenome packaging is the crucial step for maturation of plant viruses containing an RNA genome. Viruses exhibit a remarkable degree of packaging specificity, despite the probability of co-packaging cellular RNAs. Three different types of viral genome packaging systems are reported so far. The recently upgraded type I genome packaging system involves nucleation and encapsidation of RNA genomes in an energy-dependent manner, which have been observed in most of the plant RNA viruses with a smaller genome size, while type II and III packaging systems, majorly discovered in bacteriophages and large eukaryotic DNA viruses, involve genome translocation and packaging inside the prohead in an energy-dependent manner, i.e., utilizing ATP. Although ATP is essential for all three packaging systems, each machinery system employs a unique mode of ATP hydrolysis and genome packaging mechanism. Plant RNA viruses are serious threats to agricultural and horticultural crops and account for huge economic losses. Developing control strategies against plant RNA viruses requires a deep understanding of their genome assembly and packaging mechanism. On the basis of our previous studies and meticulously planned experiments, we have revealed their molecular mechanisms and proposed a hypothetical model for the type I packaging system with an emphasis on smaller plant RNA viruses. Here, in this review, we apprise researchers the technical breakthroughs that have facilitated the dissection of genome packaging and virion assembly processes in plant RNA viruses.https://www.frontiersin.org/articles/10.3389/fgene.2023.1198647/fullviral genome packagingenergy-dependentcapsid proteinATPase foldvirus assemblyRNA virus |
| spellingShingle | Tushar Ranjan Ravi Ranjan Kumar Mohammad Ansar Jitesh Kumar Auroshikha Mohanty Anamika Kumari Khushbu Jain Kumari Rajani Sailabala Dei Mohammad Feza Ahmad The curious case of genome packaging and assembly in RNA viruses infecting plants Frontiers in Genetics viral genome packaging energy-dependent capsid protein ATPase fold virus assembly RNA virus |
| title | The curious case of genome packaging and assembly in RNA viruses infecting plants |
| title_full | The curious case of genome packaging and assembly in RNA viruses infecting plants |
| title_fullStr | The curious case of genome packaging and assembly in RNA viruses infecting plants |
| title_full_unstemmed | The curious case of genome packaging and assembly in RNA viruses infecting plants |
| title_short | The curious case of genome packaging and assembly in RNA viruses infecting plants |
| title_sort | curious case of genome packaging and assembly in rna viruses infecting plants |
| topic | viral genome packaging energy-dependent capsid protein ATPase fold virus assembly RNA virus |
| url | https://www.frontiersin.org/articles/10.3389/fgene.2023.1198647/full |
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