Genetic characterization of KHM-1 metallo-β-lactamase-producing Enterobacterales isolates from inpatient sources in Osaka, Japan
ABSTRACT: Objectives: KHM-1-metallo-β-lactamase-producing Enterobacterales strains, of which only a few have been found, were isolated from four inpatients in Osaka, Japan during 2016 to 2020. We compared whole genomes of the four KHM-1-producing isolates, including one Enterobacter hormaechei subs...
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| Format: | Article |
| Language: | English |
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Elsevier
2024-06-01
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| Series: | Journal of Global Antimicrobial Resistance |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2213716524000456 |
| _version_ | 1797238085581275136 |
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| author | Kaoru Umeda Masaki Anraku Takahiro Yamaguchi Hiromi Nakamura Ryuji Kawahara |
| author_facet | Kaoru Umeda Masaki Anraku Takahiro Yamaguchi Hiromi Nakamura Ryuji Kawahara |
| author_sort | Kaoru Umeda |
| collection | DOAJ |
| description | ABSTRACT: Objectives: KHM-1-metallo-β-lactamase-producing Enterobacterales strains, of which only a few have been found, were isolated from four inpatients in Osaka, Japan during 2016 to 2020. We compared whole genomes of the four KHM-1-producing isolates, including one Enterobacter hormaechei subsp. hoffmannii, one Escherichia coli, and two Citrobacter freundii. Methods: These isolates were characterized by whole-genome sequencing, comparative analysis of blaKHM-1-encoding plasmids with earlier reported plasmids, and antimicrobial susceptibility tests. Results: Multilocus sequence typing classified the E. hormaechei subsp. hoffmannii isolate to ST78, the E. coli isolate to ST354, and the two C. freundii isolates to ST95. These isolates harboured various antimicrobial resistance genes aside from blaKHM-1 on their chromosomes and plasmids. In all four isolates, blaKHM-1 was located on 137 kbp to 213 kbp plasmids of IncC replicon type. Although there were common resistance genes such as blaKHM-1-ISEc68, class I integron cassette, and fosG, the four blaKHM-1-encoding plasmids were distinguishable into two lineages based on differences of the resistance gene components and their surrounding regions. Conclusion: Because no epidemiological contact was observed among the inpatients, the blaKHM-1-encoding IncC plasmids might have spread horizontally to multiple bacterial species through repeated recombination and insertion. |
| first_indexed | 2024-04-24T17:30:02Z |
| format | Article |
| id | doaj.art-ff2b0908cbe74bd781555c837991c25b |
| institution | Directory Open Access Journal |
| issn | 2213-7165 |
| language | English |
| last_indexed | 2024-04-24T17:30:02Z |
| publishDate | 2024-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Global Antimicrobial Resistance |
| spelling | doaj.art-ff2b0908cbe74bd781555c837991c25b2024-03-28T06:37:51ZengElsevierJournal of Global Antimicrobial Resistance2213-71652024-06-01374852Genetic characterization of KHM-1 metallo-β-lactamase-producing Enterobacterales isolates from inpatient sources in Osaka, JapanKaoru Umeda0Masaki Anraku1Takahiro Yamaguchi2Hiromi Nakamura3Ryuji Kawahara4Corresponding author.; Division of Microbiology, Bacteriology Section, Osaka Institute of Public Health, Nakamichi, Osaka, JapanDivision of Microbiology, Bacteriology Section, Osaka Institute of Public Health, Nakamichi, Osaka, JapanDivision of Microbiology, Bacteriology Section, Osaka Institute of Public Health, Nakamichi, Osaka, JapanDivision of Microbiology, Bacteriology Section, Osaka Institute of Public Health, Nakamichi, Osaka, JapanDivision of Microbiology, Bacteriology Section, Osaka Institute of Public Health, Nakamichi, Osaka, JapanABSTRACT: Objectives: KHM-1-metallo-β-lactamase-producing Enterobacterales strains, of which only a few have been found, were isolated from four inpatients in Osaka, Japan during 2016 to 2020. We compared whole genomes of the four KHM-1-producing isolates, including one Enterobacter hormaechei subsp. hoffmannii, one Escherichia coli, and two Citrobacter freundii. Methods: These isolates were characterized by whole-genome sequencing, comparative analysis of blaKHM-1-encoding plasmids with earlier reported plasmids, and antimicrobial susceptibility tests. Results: Multilocus sequence typing classified the E. hormaechei subsp. hoffmannii isolate to ST78, the E. coli isolate to ST354, and the two C. freundii isolates to ST95. These isolates harboured various antimicrobial resistance genes aside from blaKHM-1 on their chromosomes and plasmids. In all four isolates, blaKHM-1 was located on 137 kbp to 213 kbp plasmids of IncC replicon type. Although there were common resistance genes such as blaKHM-1-ISEc68, class I integron cassette, and fosG, the four blaKHM-1-encoding plasmids were distinguishable into two lineages based on differences of the resistance gene components and their surrounding regions. Conclusion: Because no epidemiological contact was observed among the inpatients, the blaKHM-1-encoding IncC plasmids might have spread horizontally to multiple bacterial species through repeated recombination and insertion.http://www.sciencedirect.com/science/article/pii/S2213716524000456KHM-1blaKHM-1IncC plasmidMetallo-β-lactamaseMultidrug resistanceWhole-genome analysis |
| spellingShingle | Kaoru Umeda Masaki Anraku Takahiro Yamaguchi Hiromi Nakamura Ryuji Kawahara Genetic characterization of KHM-1 metallo-β-lactamase-producing Enterobacterales isolates from inpatient sources in Osaka, Japan Journal of Global Antimicrobial Resistance KHM-1 blaKHM-1 IncC plasmid Metallo-β-lactamase Multidrug resistance Whole-genome analysis |
| title | Genetic characterization of KHM-1 metallo-β-lactamase-producing Enterobacterales isolates from inpatient sources in Osaka, Japan |
| title_full | Genetic characterization of KHM-1 metallo-β-lactamase-producing Enterobacterales isolates from inpatient sources in Osaka, Japan |
| title_fullStr | Genetic characterization of KHM-1 metallo-β-lactamase-producing Enterobacterales isolates from inpatient sources in Osaka, Japan |
| title_full_unstemmed | Genetic characterization of KHM-1 metallo-β-lactamase-producing Enterobacterales isolates from inpatient sources in Osaka, Japan |
| title_short | Genetic characterization of KHM-1 metallo-β-lactamase-producing Enterobacterales isolates from inpatient sources in Osaka, Japan |
| title_sort | genetic characterization of khm 1 metallo β lactamase producing enterobacterales isolates from inpatient sources in osaka japan |
| topic | KHM-1 blaKHM-1 IncC plasmid Metallo-β-lactamase Multidrug resistance Whole-genome analysis |
| url | http://www.sciencedirect.com/science/article/pii/S2213716524000456 |
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