Application and Expansion of Virus-Induced Gene Silencing for Functional Studies in Vegetables
Increased consumption of vegetables has been recommended worldwide as a part of a healthy diet; therefore, determining gene function among breeding materials is crucial for vegetable improvement to meet the sustainable development of new vegetable varieties. However, genetic transformation is time-c...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-08-01
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| Series: | Horticulturae |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2311-7524/9/8/934 |
| _version_ | 1797584598817832960 |
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| author | Zheng Wang Shoujun Cao Xinyang Xu Yanjun He Weisong Shou Eduardo D. Munaiz Chao Yu Jia Shen |
| author_facet | Zheng Wang Shoujun Cao Xinyang Xu Yanjun He Weisong Shou Eduardo D. Munaiz Chao Yu Jia Shen |
| author_sort | Zheng Wang |
| collection | DOAJ |
| description | Increased consumption of vegetables has been recommended worldwide as a part of a healthy diet; therefore, determining gene function among breeding materials is crucial for vegetable improvement to meet the sustainable development of new vegetable varieties. However, genetic transformation is time-consuming and laborious, which limits the exploration of gene function for various vegetable crops. Virus-Induced Gene Silencing (VIGS) can perform large-scale and rapid gene silencing in plants due to a reduction in the experimental period and its independence from the stable genetic transformation, providing an excellent opportunity for functional research. VIGS can accelerate model plant research and make it easier to analyze gene function and validation in vegetable crops. Moreover, with the advent of technologies such as virus-mediated heterologous protein expression and the development of CRISPR/Cas9 technology, virus-mediated genetic tools have ushered in a new era in genetics and crop improvement. This study summarizes recent achievements in VIGS and Virus-Induced Gene Editing (VIGE) in vegetables. We also identify several challenges in the current state of VIGS technology in vegetables, serving as a guide for future research. |
| first_indexed | 2024-03-10T23:53:56Z |
| format | Article |
| id | doaj.art-ff2b39acbce5468f9416129cf16daf4e |
| institution | Directory Open Access Journal |
| issn | 2311-7524 |
| language | English |
| last_indexed | 2024-03-10T23:53:56Z |
| publishDate | 2023-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Horticulturae |
| spelling | doaj.art-ff2b39acbce5468f9416129cf16daf4e2023-11-19T01:22:00ZengMDPI AGHorticulturae2311-75242023-08-019893410.3390/horticulturae9080934Application and Expansion of Virus-Induced Gene Silencing for Functional Studies in VegetablesZheng Wang0Shoujun Cao1Xinyang Xu2Yanjun He3Weisong Shou4Eduardo D. Munaiz5Chao Yu6Jia Shen7College of Horticulture Science, Zhejiang Agriculture and Forestry University, Hangzhou 311300, ChinaYantai Agricultural Science Academy of Shandong Province, Yantai 265500, ChinaZhejiang Academy of Agricultural Sciences, Hangzhou 310021, ChinaZhejiang Academy of Agricultural Sciences, Hangzhou 310021, ChinaZhejiang Academy of Agricultural Sciences, Hangzhou 310021, ChinaUniLaSalle, AGHYLE UP 2018.C101, 60000 Beauvais, FranceCollege of Horticulture Science, Zhejiang Agriculture and Forestry University, Hangzhou 311300, ChinaZhejiang Academy of Agricultural Sciences, Hangzhou 310021, ChinaIncreased consumption of vegetables has been recommended worldwide as a part of a healthy diet; therefore, determining gene function among breeding materials is crucial for vegetable improvement to meet the sustainable development of new vegetable varieties. However, genetic transformation is time-consuming and laborious, which limits the exploration of gene function for various vegetable crops. Virus-Induced Gene Silencing (VIGS) can perform large-scale and rapid gene silencing in plants due to a reduction in the experimental period and its independence from the stable genetic transformation, providing an excellent opportunity for functional research. VIGS can accelerate model plant research and make it easier to analyze gene function and validation in vegetable crops. Moreover, with the advent of technologies such as virus-mediated heterologous protein expression and the development of CRISPR/Cas9 technology, virus-mediated genetic tools have ushered in a new era in genetics and crop improvement. This study summarizes recent achievements in VIGS and Virus-Induced Gene Editing (VIGE) in vegetables. We also identify several challenges in the current state of VIGS technology in vegetables, serving as a guide for future research.https://www.mdpi.com/2311-7524/9/8/934VIGSVIGEVIGOCRISPR/Cas9VSRs |
| spellingShingle | Zheng Wang Shoujun Cao Xinyang Xu Yanjun He Weisong Shou Eduardo D. Munaiz Chao Yu Jia Shen Application and Expansion of Virus-Induced Gene Silencing for Functional Studies in Vegetables Horticulturae VIGS VIGE VIGO CRISPR/Cas9 VSRs |
| title | Application and Expansion of Virus-Induced Gene Silencing for Functional Studies in Vegetables |
| title_full | Application and Expansion of Virus-Induced Gene Silencing for Functional Studies in Vegetables |
| title_fullStr | Application and Expansion of Virus-Induced Gene Silencing for Functional Studies in Vegetables |
| title_full_unstemmed | Application and Expansion of Virus-Induced Gene Silencing for Functional Studies in Vegetables |
| title_short | Application and Expansion of Virus-Induced Gene Silencing for Functional Studies in Vegetables |
| title_sort | application and expansion of virus induced gene silencing for functional studies in vegetables |
| topic | VIGS VIGE VIGO CRISPR/Cas9 VSRs |
| url | https://www.mdpi.com/2311-7524/9/8/934 |
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