Th17/Treg-Related Intracellular Signaling in Patients with Chronic Obstructive Pulmonary Disease: Comparison between Local and Systemic Responses
Th17/Treg imbalance plays a pivotal role in COPD development and progression. We aimed to assess Th17/Treg-related intracellular signaling at different COPD stages in local and systemic responses. Lung tissue and/or peripheral blood samples were collected and divided into non-obstructed (NOS), COPD...
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MDPI AG
2021-06-01
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| author | Juliana D. Lourenço Walcy R. Teodoro Denise F. Barbeiro Ana Paula P. Velosa Larissa E. F. Silva Júlia B. Kohler Alyne R. Moreira Marcelo V. Aun Isadora C. da Silva Frederico L. A. Fernandes Elnara M. Negri Jefferson L. Gross Iolanda F. L. C. Tibério Juliana T. Ito Fernanda D. T. Q. S. Lopes |
| author_facet | Juliana D. Lourenço Walcy R. Teodoro Denise F. Barbeiro Ana Paula P. Velosa Larissa E. F. Silva Júlia B. Kohler Alyne R. Moreira Marcelo V. Aun Isadora C. da Silva Frederico L. A. Fernandes Elnara M. Negri Jefferson L. Gross Iolanda F. L. C. Tibério Juliana T. Ito Fernanda D. T. Q. S. Lopes |
| author_sort | Juliana D. Lourenço |
| collection | DOAJ |
| description | Th17/Treg imbalance plays a pivotal role in COPD development and progression. We aimed to assess Th17/Treg-related intracellular signaling at different COPD stages in local and systemic responses. Lung tissue and/or peripheral blood samples were collected and divided into non-obstructed (NOS), COPD stages I and II, and COPD stages III and IV groups. Gene expression of <i>STAT3</i> and <i>-5</i>, <i>RORγt</i>, <i>Foxp3</i>, interleukin <i>(IL)-6</i>, <i>-17</i>, <i>-10</i>, and <i>TGF-β</i> was assessed by RT-qPCR. IL-6, -17, -10, and TGF-β levels were determined by ELISA. We observed increased <i>STAT3</i>, <i>RORγt</i>, <i>Foxp3</i>, <i>IL-6</i>, and <i>TGF-β</i> gene expression and IL-6 levels in the lungs of COPD I and II patients compared to those of NOS patients. Regarding the systemic response, we observed increased <i>STAT3</i>, <i>RORγt</i>, <i>IL-6</i>, and <i>TGF-β</i> gene expression in the COPD III and IV group and increased IL-6 levels in the COPD I and II group. <i>STAT5</i> was increased in COPD III and IV patients, although there was a decrease in <i>Foxp3</i> expression and IL-10 levels in the COPD I and II and COPD III and IV groups, respectively. We demonstrated that an increase in Th17 intracellular signaling in the lungs precedes this increase in the systemic response, whereas Treg intracellular signaling varies between the compartments analyzed in different COPD stages. |
| first_indexed | 2024-03-10T10:11:16Z |
| format | Article |
| id | doaj.art-ff2e7bdbdf294f9ba38c9d1992c3ae28 |
| institution | Directory Open Access Journal |
| issn | 2073-4409 |
| language | English |
| last_indexed | 2024-03-10T10:11:16Z |
| publishDate | 2021-06-01 |
| publisher | MDPI AG |
| record_format | Article |
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| spelling | doaj.art-ff2e7bdbdf294f9ba38c9d1992c3ae282023-11-22T01:11:56ZengMDPI AGCells2073-44092021-06-01107156910.3390/cells10071569Th17/Treg-Related Intracellular Signaling in Patients with Chronic Obstructive Pulmonary Disease: Comparison between Local and Systemic ResponsesJuliana D. Lourenço0Walcy R. Teodoro1Denise F. Barbeiro2Ana Paula P. Velosa3Larissa E. F. Silva4Júlia B. Kohler5Alyne R. Moreira6Marcelo V. Aun7Isadora C. da Silva8Frederico L. A. Fernandes9Elnara M. Negri10Jefferson L. Gross11Iolanda F. L. C. Tibério12Juliana T. Ito13Fernanda D. T. Q. S. Lopes14Laboratory of Experimental Therapeutics, Department of Clinical Medicine, School of Medicine, University of Sao Paulo, Av. Dr. Arnaldo 455—Room 1220, Sao Paulo 01246-903, BrazilRheumatology Division of the Hospital das Clinicas FMUSP, Faculdade de Medicina, Universidade de Sao Paulo, Av. Dr. Arnaldo 455—Room 3124, Sao Paulo 01246-903, BrazilLaboratory of Clinical Emergencies, Department of Clinical Medicine, School of Medicine, University of Sao Paulo, Av. Dr. Arnaldo 455—Room 3132, Sao Paulo 01246-903, BrazilRheumatology Division of the Hospital das Clinicas FMUSP, Faculdade de Medicina, Universidade de Sao Paulo, Av. Dr. Arnaldo 455—Room 3124, Sao Paulo 01246-903, BrazilLaboratory of Experimental Therapeutics, Department of Clinical Medicine, School of Medicine, University of Sao Paulo, Av. Dr. Arnaldo 455—Room 1220, Sao Paulo 01246-903, BrazilLaboratory of Experimental Therapeutics, Department of Clinical Medicine, School of Medicine, University of Sao Paulo, Av. Dr. Arnaldo 455—Room 1220, Sao Paulo 01246-903, BrazilLaboratory of Experimental Therapeutics, Department of Clinical Medicine, School of Medicine, University of Sao Paulo, Av. Dr. Arnaldo 455—Room 1220, Sao Paulo 01246-903, BrazilHost & Defense Unit, Faculdade Israelita de Ciências da Saúde Albert Einstein School of Medicine, Av. Prof. Francisco Morato 4.293, Sao Paulo 05521-200, BrazilPulmonary Division—Heart Institute of Hospital das Clinicas, School of Medicine of University of Sao Paulo, Av. Dr. Enéas Carvalho Aguiar 44, Sao Paulo 05403-000, BrazilPulmonary Division—Heart Institute of Hospital das Clinicas, School of Medicine of University of Sao Paulo, Av. Dr. Enéas Carvalho Aguiar 44, Sao Paulo 05403-000, BrazilLaboratory of Cellular Biology, Department of Pathology, School of Medicine, University of Sao Paulo, Av. Dr. Arnaldo 455—Room 4349, Sao Paulo 01246-903, BrazilDepartment of Thoracic Surgery, A C Camargo Cancer Center, R. Prof Antonio Prudente 211, Sao Paulo 01509-010, BrazilLaboratory of Experimental Therapeutics, Department of Clinical Medicine, School of Medicine, University of Sao Paulo, Av. Dr. Arnaldo 455—Room 1220, Sao Paulo 01246-903, BrazilLaboratory of Experimental Therapeutics, Department of Clinical Medicine, School of Medicine, University of Sao Paulo, Av. Dr. Arnaldo 455—Room 1220, Sao Paulo 01246-903, BrazilLaboratory of Experimental Therapeutics, Department of Clinical Medicine, School of Medicine, University of Sao Paulo, Av. Dr. Arnaldo 455—Room 1220, Sao Paulo 01246-903, BrazilTh17/Treg imbalance plays a pivotal role in COPD development and progression. We aimed to assess Th17/Treg-related intracellular signaling at different COPD stages in local and systemic responses. Lung tissue and/or peripheral blood samples were collected and divided into non-obstructed (NOS), COPD stages I and II, and COPD stages III and IV groups. Gene expression of <i>STAT3</i> and <i>-5</i>, <i>RORγt</i>, <i>Foxp3</i>, interleukin <i>(IL)-6</i>, <i>-17</i>, <i>-10</i>, and <i>TGF-β</i> was assessed by RT-qPCR. IL-6, -17, -10, and TGF-β levels were determined by ELISA. We observed increased <i>STAT3</i>, <i>RORγt</i>, <i>Foxp3</i>, <i>IL-6</i>, and <i>TGF-β</i> gene expression and IL-6 levels in the lungs of COPD I and II patients compared to those of NOS patients. Regarding the systemic response, we observed increased <i>STAT3</i>, <i>RORγt</i>, <i>IL-6</i>, and <i>TGF-β</i> gene expression in the COPD III and IV group and increased IL-6 levels in the COPD I and II group. <i>STAT5</i> was increased in COPD III and IV patients, although there was a decrease in <i>Foxp3</i> expression and IL-10 levels in the COPD I and II and COPD III and IV groups, respectively. We demonstrated that an increase in Th17 intracellular signaling in the lungs precedes this increase in the systemic response, whereas Treg intracellular signaling varies between the compartments analyzed in different COPD stages.https://www.mdpi.com/2073-4409/10/7/1569COPDTregTh17STATintracellular signaling |
| spellingShingle | Juliana D. Lourenço Walcy R. Teodoro Denise F. Barbeiro Ana Paula P. Velosa Larissa E. F. Silva Júlia B. Kohler Alyne R. Moreira Marcelo V. Aun Isadora C. da Silva Frederico L. A. Fernandes Elnara M. Negri Jefferson L. Gross Iolanda F. L. C. Tibério Juliana T. Ito Fernanda D. T. Q. S. Lopes Th17/Treg-Related Intracellular Signaling in Patients with Chronic Obstructive Pulmonary Disease: Comparison between Local and Systemic Responses Cells COPD Treg Th17 STAT intracellular signaling |
| title | Th17/Treg-Related Intracellular Signaling in Patients with Chronic Obstructive Pulmonary Disease: Comparison between Local and Systemic Responses |
| title_full | Th17/Treg-Related Intracellular Signaling in Patients with Chronic Obstructive Pulmonary Disease: Comparison between Local and Systemic Responses |
| title_fullStr | Th17/Treg-Related Intracellular Signaling in Patients with Chronic Obstructive Pulmonary Disease: Comparison between Local and Systemic Responses |
| title_full_unstemmed | Th17/Treg-Related Intracellular Signaling in Patients with Chronic Obstructive Pulmonary Disease: Comparison between Local and Systemic Responses |
| title_short | Th17/Treg-Related Intracellular Signaling in Patients with Chronic Obstructive Pulmonary Disease: Comparison between Local and Systemic Responses |
| title_sort | th17 treg related intracellular signaling in patients with chronic obstructive pulmonary disease comparison between local and systemic responses |
| topic | COPD Treg Th17 STAT intracellular signaling |
| url | https://www.mdpi.com/2073-4409/10/7/1569 |
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