High-performance liquid chromatography ultraviolet-photodiode array detection method for aflatoxin B1 in cattle feed supplements
Aim: The objective of the current study is to determine the concentration of aflatoxin B1 using high-performance liquid chromatography (HPLC) with a photodiode array (PDA) detector. Materials and Methods: Aflatoxin B1 certified reference grade from Trilogy Analytical Laboratory dissolved acetonit...
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| Format: | Article |
| Language: | English |
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Veterinary World
2017-08-01
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| Series: | Veterinary World |
| Subjects: | |
| Online Access: | http://www.veterinaryworld.org/Vol.10/August-2017/17.pdf |
| _version_ | 1818621991149633536 |
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| author | Lazuardi Mochamad Bambang Hermanto |
| author_facet | Lazuardi Mochamad Bambang Hermanto |
| author_sort | Lazuardi Mochamad |
| collection | DOAJ |
| description | Aim: The objective of the current study is to determine the concentration of aflatoxin B1 using high-performance liquid chromatography (HPLC) with a photodiode array (PDA) detector.
Materials and Methods: Aflatoxin B1 certified reference grade from Trilogy Analytical Laboratory dissolved acetonitrile (ACN) at 10 μg/mL was used for standard assessment. HPLC instruments such as ultraviolet-PDA detector used a Shimadzu LC-6AD pump with DGU-20A5 degasser, communication module-20A, and PDA detector SPD-M20A with FRC-10A fraction collector. The HPLC was set isocratic method at 354 nm with a reverse-phase ODS C18 column (LiChrospher® 100 RP-18; diameter, 5 μm) under a 20°C controlled column chamber. Rheodyne® sample loops were performed in 20 μL capacities. The mobile phase was performed at fraction 63:26:11 H2O: methanol:ACN at pH 6.8. A total of 1 kg of feed contained 10% bread crumbs and 30% concentrated, 40% forage, and 20% soybean dregs were using commercials samples. Samples were extracted by ACN and separated with solid phase extraction ODS 1 mL than elution with mobile phase to collect at drying samples performed. The samples were ready to use after added 1 mL mobile phase than injected into the system of HPLC.
Results: We found that the retention time of aflatoxin B1 was approximately 10.858 min. Linearity of 0.01-0.08 μg/mL aflatoxin B1 dissolved in mobile phase was obtained at R2=0.9. These results demonstrate that these methods can be used to analyze aflatoxin B1 and gain 89-99% recovery. The limit of detection of this assay was obtained at 3.5 x 10-6 μg/mL.
Conclusion: This method was easy to apply and suitable to analyzing at small concentrations of aflatoxin B1 in formulated product of feed cattle. |
| first_indexed | 2024-12-16T18:18:04Z |
| format | Article |
| id | doaj.art-ff2ff99e6c29401db5d12a78edd53445 |
| institution | Directory Open Access Journal |
| issn | 0972-8988 2231-0916 |
| language | English |
| last_indexed | 2024-12-16T18:18:04Z |
| publishDate | 2017-08-01 |
| publisher | Veterinary World |
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| series | Veterinary World |
| spelling | doaj.art-ff2ff99e6c29401db5d12a78edd534452022-12-21T22:21:38ZengVeterinary WorldVeterinary World0972-89882231-09162017-08-0110893293810.14202/vetworld.2017.932-938High-performance liquid chromatography ultraviolet-photodiode array detection method for aflatoxin B1 in cattle feed supplementsLazuardi Mochamad0Bambang Hermanto1Department of Basic Science, Veterinary Pharmacy Subdivision, Faculty of Veterinary Medicine, Airlangga University, Surabaya, Indonesia.Department of Pharmacology, Medical Faculty Airlangga University, Prof. Dr. Moestopo 47, Pacar Kembang, Surabaya, Indonesia.Aim: The objective of the current study is to determine the concentration of aflatoxin B1 using high-performance liquid chromatography (HPLC) with a photodiode array (PDA) detector. Materials and Methods: Aflatoxin B1 certified reference grade from Trilogy Analytical Laboratory dissolved acetonitrile (ACN) at 10 μg/mL was used for standard assessment. HPLC instruments such as ultraviolet-PDA detector used a Shimadzu LC-6AD pump with DGU-20A5 degasser, communication module-20A, and PDA detector SPD-M20A with FRC-10A fraction collector. The HPLC was set isocratic method at 354 nm with a reverse-phase ODS C18 column (LiChrospher® 100 RP-18; diameter, 5 μm) under a 20°C controlled column chamber. Rheodyne® sample loops were performed in 20 μL capacities. The mobile phase was performed at fraction 63:26:11 H2O: methanol:ACN at pH 6.8. A total of 1 kg of feed contained 10% bread crumbs and 30% concentrated, 40% forage, and 20% soybean dregs were using commercials samples. Samples were extracted by ACN and separated with solid phase extraction ODS 1 mL than elution with mobile phase to collect at drying samples performed. The samples were ready to use after added 1 mL mobile phase than injected into the system of HPLC. Results: We found that the retention time of aflatoxin B1 was approximately 10.858 min. Linearity of 0.01-0.08 μg/mL aflatoxin B1 dissolved in mobile phase was obtained at R2=0.9. These results demonstrate that these methods can be used to analyze aflatoxin B1 and gain 89-99% recovery. The limit of detection of this assay was obtained at 3.5 x 10-6 μg/mL. Conclusion: This method was easy to apply and suitable to analyzing at small concentrations of aflatoxin B1 in formulated product of feed cattle.http://www.veterinaryworld.org/Vol.10/August-2017/17.pdffeeds supplementshigh-performance liquid chromatography photodiode array detectorisocratic methodsmycotoxin |
| spellingShingle | Lazuardi Mochamad Bambang Hermanto High-performance liquid chromatography ultraviolet-photodiode array detection method for aflatoxin B1 in cattle feed supplements Veterinary World feeds supplements high-performance liquid chromatography photodiode array detector isocratic methods mycotoxin |
| title | High-performance liquid chromatography ultraviolet-photodiode array detection method for aflatoxin B1 in cattle feed supplements |
| title_full | High-performance liquid chromatography ultraviolet-photodiode array detection method for aflatoxin B1 in cattle feed supplements |
| title_fullStr | High-performance liquid chromatography ultraviolet-photodiode array detection method for aflatoxin B1 in cattle feed supplements |
| title_full_unstemmed | High-performance liquid chromatography ultraviolet-photodiode array detection method for aflatoxin B1 in cattle feed supplements |
| title_short | High-performance liquid chromatography ultraviolet-photodiode array detection method for aflatoxin B1 in cattle feed supplements |
| title_sort | high performance liquid chromatography ultraviolet photodiode array detection method for aflatoxin b1 in cattle feed supplements |
| topic | feeds supplements high-performance liquid chromatography photodiode array detector isocratic methods mycotoxin |
| url | http://www.veterinaryworld.org/Vol.10/August-2017/17.pdf |
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