Peripheral B cells from patients with hepatitis C virus-associated lymphoma exhibit clonal expansion and an anergic-like transcriptional profile
Summary: Chronic HCV infection remains a global health concern due to its involvement in hepatic and extrahepatic diseases, including B cell non-Hodgkin lymphoma (BNHL). Clinical and epidemiological evidence support a causal role for HCV in BNHL development, although mechanistic insight is lacking....
| Main Authors: | , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2023-01-01
|
| Series: | iScience |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2589004222020740 |
| _version_ | 1797946199358046208 |
|---|---|
| author | Amanda N. Henning Myagmarjav Budeebazar Delgerbat Boldbaatar Dahgwahdorj Yagaanbuyant Davaadorj Duger Khishigjargal Batsukh Huizhi Zhou Ryan Baumann Robert D. Allison Harvey J. Alter Naranjargal Dashdorj Valeria De Giorgi |
| author_facet | Amanda N. Henning Myagmarjav Budeebazar Delgerbat Boldbaatar Dahgwahdorj Yagaanbuyant Davaadorj Duger Khishigjargal Batsukh Huizhi Zhou Ryan Baumann Robert D. Allison Harvey J. Alter Naranjargal Dashdorj Valeria De Giorgi |
| author_sort | Amanda N. Henning |
| collection | DOAJ |
| description | Summary: Chronic HCV infection remains a global health concern due to its involvement in hepatic and extrahepatic diseases, including B cell non-Hodgkin lymphoma (BNHL). Clinical and epidemiological evidence support a causal role for HCV in BNHL development, although mechanistic insight is lacking. We performed RNA-sequencing on peripheral B cells from patients with HCV alone, BNHL alone, and HCV-associated BNHL to identify unique and shared transcriptional profiles associated with transformation. In patients with HCV-associated BNHL, we observed the enrichment of an anergic-like gene signature and evidence of clonal expansion that was correlated with the expression of epigenetic regulatory genes. Our data support a role for viral-mediated clonal expansion of anergic-like B cells in HCV-associated BNHL development and suggest epigenetic dysregulation as a potential mechanism driving expansion. We propose epigenetic mechanisms may be involved in both HCV-associated lymphoma and regulation of B cell anergy, representing an attractive target for clinical interventions. |
| first_indexed | 2024-04-10T21:07:06Z |
| format | Article |
| id | doaj.art-ff30ba90c541428bb1b29db39acc5716 |
| institution | Directory Open Access Journal |
| issn | 2589-0042 |
| language | English |
| last_indexed | 2024-04-10T21:07:06Z |
| publishDate | 2023-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | iScience |
| spelling | doaj.art-ff30ba90c541428bb1b29db39acc57162023-01-22T04:41:04ZengElsevieriScience2589-00422023-01-01261105801Peripheral B cells from patients with hepatitis C virus-associated lymphoma exhibit clonal expansion and an anergic-like transcriptional profileAmanda N. Henning0Myagmarjav Budeebazar1Delgerbat Boldbaatar2Dahgwahdorj Yagaanbuyant3Davaadorj Duger4Khishigjargal Batsukh5Huizhi Zhou6Ryan Baumann7Robert D. Allison8Harvey J. Alter9Naranjargal Dashdorj10Valeria De Giorgi11Department of Transfusion Medicine, Clinical Center, National Institutes of Health, Bethesda, MD 20892, USA; Corresponding authorDepartment of Gastroenterology, Mongolian National University of Medical Sciences, Ulaanbaatar 14210, Mongolia; Liver Center, Ulaanbaatar 14230, MongoliaLiver Center, Ulaanbaatar 14230, MongoliaLiver Center, Ulaanbaatar 14230, MongoliaDepartment of Gastroenterology, Mongolian National University of Medical Sciences, Ulaanbaatar 14210, MongoliaCenter of Hematology and Bone Marrow Transplantation, First Central Hospital of Mongolia, Ulaanbaatar 14210, MongoliaDepartment of Transfusion Medicine, Clinical Center, National Institutes of Health, Bethesda, MD 20892, USADepartment of Transfusion Medicine, Clinical Center, National Institutes of Health, Bethesda, MD 20892, USABloomberg School of Public Health, Johns Hopkins University, Baltimore, MD 21205, USADepartment of Transfusion Medicine, Clinical Center, National Institutes of Health, Bethesda, MD 20892, USALiver Center, Ulaanbaatar 14230, Mongolia; Onom Foundation, Ulaanbaatar 17011, MongoliaDepartment of Transfusion Medicine, Clinical Center, National Institutes of Health, Bethesda, MD 20892, USA; Corresponding authorSummary: Chronic HCV infection remains a global health concern due to its involvement in hepatic and extrahepatic diseases, including B cell non-Hodgkin lymphoma (BNHL). Clinical and epidemiological evidence support a causal role for HCV in BNHL development, although mechanistic insight is lacking. We performed RNA-sequencing on peripheral B cells from patients with HCV alone, BNHL alone, and HCV-associated BNHL to identify unique and shared transcriptional profiles associated with transformation. In patients with HCV-associated BNHL, we observed the enrichment of an anergic-like gene signature and evidence of clonal expansion that was correlated with the expression of epigenetic regulatory genes. Our data support a role for viral-mediated clonal expansion of anergic-like B cells in HCV-associated BNHL development and suggest epigenetic dysregulation as a potential mechanism driving expansion. We propose epigenetic mechanisms may be involved in both HCV-associated lymphoma and regulation of B cell anergy, representing an attractive target for clinical interventions.http://www.sciencedirect.com/science/article/pii/S2589004222020740EpigeneticsImmunologyVirology |
| spellingShingle | Amanda N. Henning Myagmarjav Budeebazar Delgerbat Boldbaatar Dahgwahdorj Yagaanbuyant Davaadorj Duger Khishigjargal Batsukh Huizhi Zhou Ryan Baumann Robert D. Allison Harvey J. Alter Naranjargal Dashdorj Valeria De Giorgi Peripheral B cells from patients with hepatitis C virus-associated lymphoma exhibit clonal expansion and an anergic-like transcriptional profile iScience Epigenetics Immunology Virology |
| title | Peripheral B cells from patients with hepatitis C virus-associated lymphoma exhibit clonal expansion and an anergic-like transcriptional profile |
| title_full | Peripheral B cells from patients with hepatitis C virus-associated lymphoma exhibit clonal expansion and an anergic-like transcriptional profile |
| title_fullStr | Peripheral B cells from patients with hepatitis C virus-associated lymphoma exhibit clonal expansion and an anergic-like transcriptional profile |
| title_full_unstemmed | Peripheral B cells from patients with hepatitis C virus-associated lymphoma exhibit clonal expansion and an anergic-like transcriptional profile |
| title_short | Peripheral B cells from patients with hepatitis C virus-associated lymphoma exhibit clonal expansion and an anergic-like transcriptional profile |
| title_sort | peripheral b cells from patients with hepatitis c virus associated lymphoma exhibit clonal expansion and an anergic like transcriptional profile |
| topic | Epigenetics Immunology Virology |
| url | http://www.sciencedirect.com/science/article/pii/S2589004222020740 |
| work_keys_str_mv | AT amandanhenning peripheralbcellsfrompatientswithhepatitiscvirusassociatedlymphomaexhibitclonalexpansionandananergicliketranscriptionalprofile AT myagmarjavbudeebazar peripheralbcellsfrompatientswithhepatitiscvirusassociatedlymphomaexhibitclonalexpansionandananergicliketranscriptionalprofile AT delgerbatboldbaatar peripheralbcellsfrompatientswithhepatitiscvirusassociatedlymphomaexhibitclonalexpansionandananergicliketranscriptionalprofile AT dahgwahdorjyagaanbuyant peripheralbcellsfrompatientswithhepatitiscvirusassociatedlymphomaexhibitclonalexpansionandananergicliketranscriptionalprofile AT davaadorjduger peripheralbcellsfrompatientswithhepatitiscvirusassociatedlymphomaexhibitclonalexpansionandananergicliketranscriptionalprofile AT khishigjargalbatsukh peripheralbcellsfrompatientswithhepatitiscvirusassociatedlymphomaexhibitclonalexpansionandananergicliketranscriptionalprofile AT huizhizhou peripheralbcellsfrompatientswithhepatitiscvirusassociatedlymphomaexhibitclonalexpansionandananergicliketranscriptionalprofile AT ryanbaumann peripheralbcellsfrompatientswithhepatitiscvirusassociatedlymphomaexhibitclonalexpansionandananergicliketranscriptionalprofile AT robertdallison peripheralbcellsfrompatientswithhepatitiscvirusassociatedlymphomaexhibitclonalexpansionandananergicliketranscriptionalprofile AT harveyjalter peripheralbcellsfrompatientswithhepatitiscvirusassociatedlymphomaexhibitclonalexpansionandananergicliketranscriptionalprofile AT naranjargaldashdorj peripheralbcellsfrompatientswithhepatitiscvirusassociatedlymphomaexhibitclonalexpansionandananergicliketranscriptionalprofile AT valeriadegiorgi peripheralbcellsfrompatientswithhepatitiscvirusassociatedlymphomaexhibitclonalexpansionandananergicliketranscriptionalprofile |