Study of Androgenic Plant Families of Alloplasmic Introgression Lines (<i>H. vulgare</i>) –<i>T. aestivum</i> and the Use of Sister DH Lines in Breeding
One of the limitations in obtaining the genetic diversity of doubled haploid (DH) lines via anther culture is the development of families of regenerants, and each family represents a clone. This work examines the results of studying this phenomenon in anther culture of alloplasmic (<i>H. vulga...
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MDPI AG
2020-06-01
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| Series: | Plants |
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| Online Access: | https://www.mdpi.com/2223-7747/9/6/764 |
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| author | Lidiya Pershina Nataliya Trubacheeva Ekaterina Badaeva Igor Belan Ludmila Rosseeva |
| author_facet | Lidiya Pershina Nataliya Trubacheeva Ekaterina Badaeva Igor Belan Ludmila Rosseeva |
| author_sort | Lidiya Pershina |
| collection | DOAJ |
| description | One of the limitations in obtaining the genetic diversity of doubled haploid (DH) lines via anther culture is the development of families of regenerants, and each family represents a clone. This work examines the results of studying this phenomenon in anther culture of alloplasmic (<i>H. vulgare</i>)–<i>T. aestivum</i> and euplasmic lines with 1RS.1BL and 7DL-7Ai translocations and hybrids between them. Parameters of androgenesis such as the number of embryo-like structures, the total number of regenerants, and the number of green regenerants per 100 anthers varied depending on the genotype. In all genotypes from embryo-like structures, predominant development of families of plantlets rather than single plantlets was found. The source of family plantlets was polyembryos. About 75% of families consisted of regenerants at the same fertility level. On average, 37.74%4% of the R0 plants were fertile. The sister DH lines of three hybrid combinations were formed from seeds of R1 plants (2<i>n</i> = 42) with high fertility and in the presence of wheat–alien translocations. After four years of breeding trials, the sister DH lines of three families with fungal disease resistance increased yield, and some parameters of grain quality exceeding the controls were identified as promising for breeding. |
| first_indexed | 2024-03-10T19:03:46Z |
| format | Article |
| id | doaj.art-ff30dd482d2747e18d8aec4a681a9281 |
| institution | Directory Open Access Journal |
| issn | 2223-7747 |
| language | English |
| last_indexed | 2024-03-10T19:03:46Z |
| publishDate | 2020-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Plants |
| spelling | doaj.art-ff30dd482d2747e18d8aec4a681a92812023-11-20T04:18:37ZengMDPI AGPlants2223-77472020-06-019676410.3390/plants9060764Study of Androgenic Plant Families of Alloplasmic Introgression Lines (<i>H. vulgare</i>) –<i>T. aestivum</i> and the Use of Sister DH Lines in BreedingLidiya Pershina0Nataliya Trubacheeva1Ekaterina Badaeva2Igor Belan3Ludmila Rosseeva4Institute of Cytology and Genetics, SB RAS, 630090 Novosibirsk, RussiaInstitute of Cytology and Genetics, SB RAS, 630090 Novosibirsk, RussiaVavilov Institute of General Genetics RAS, 119991 Moscow, RussiaOmsk Agricultural Scientific Center, 644012 Omsk, RussiaOmsk Agricultural Scientific Center, 644012 Omsk, RussiaOne of the limitations in obtaining the genetic diversity of doubled haploid (DH) lines via anther culture is the development of families of regenerants, and each family represents a clone. This work examines the results of studying this phenomenon in anther culture of alloplasmic (<i>H. vulgare</i>)–<i>T. aestivum</i> and euplasmic lines with 1RS.1BL and 7DL-7Ai translocations and hybrids between them. Parameters of androgenesis such as the number of embryo-like structures, the total number of regenerants, and the number of green regenerants per 100 anthers varied depending on the genotype. In all genotypes from embryo-like structures, predominant development of families of plantlets rather than single plantlets was found. The source of family plantlets was polyembryos. About 75% of families consisted of regenerants at the same fertility level. On average, 37.74%4% of the R0 plants were fertile. The sister DH lines of three hybrid combinations were formed from seeds of R1 plants (2<i>n</i> = 42) with high fertility and in the presence of wheat–alien translocations. After four years of breeding trials, the sister DH lines of three families with fungal disease resistance increased yield, and some parameters of grain quality exceeding the controls were identified as promising for breeding.https://www.mdpi.com/2223-7747/9/6/764anther culturefamilies of regenerants(<i>H. vulgare</i>)–<i>T. aestivum</i> linesbreeding |
| spellingShingle | Lidiya Pershina Nataliya Trubacheeva Ekaterina Badaeva Igor Belan Ludmila Rosseeva Study of Androgenic Plant Families of Alloplasmic Introgression Lines (<i>H. vulgare</i>) –<i>T. aestivum</i> and the Use of Sister DH Lines in Breeding Plants anther culture families of regenerants (<i>H. vulgare</i>)–<i>T. aestivum</i> lines breeding |
| title | Study of Androgenic Plant Families of Alloplasmic Introgression Lines (<i>H. vulgare</i>) –<i>T. aestivum</i> and the Use of Sister DH Lines in Breeding |
| title_full | Study of Androgenic Plant Families of Alloplasmic Introgression Lines (<i>H. vulgare</i>) –<i>T. aestivum</i> and the Use of Sister DH Lines in Breeding |
| title_fullStr | Study of Androgenic Plant Families of Alloplasmic Introgression Lines (<i>H. vulgare</i>) –<i>T. aestivum</i> and the Use of Sister DH Lines in Breeding |
| title_full_unstemmed | Study of Androgenic Plant Families of Alloplasmic Introgression Lines (<i>H. vulgare</i>) –<i>T. aestivum</i> and the Use of Sister DH Lines in Breeding |
| title_short | Study of Androgenic Plant Families of Alloplasmic Introgression Lines (<i>H. vulgare</i>) –<i>T. aestivum</i> and the Use of Sister DH Lines in Breeding |
| title_sort | study of androgenic plant families of alloplasmic introgression lines i h vulgare i i t aestivum i and the use of sister dh lines in breeding |
| topic | anther culture families of regenerants (<i>H. vulgare</i>)–<i>T. aestivum</i> lines breeding |
| url | https://www.mdpi.com/2223-7747/9/6/764 |
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