Modulation of TNF-α plasma levels in coronary artery disease [CAD] and non-CAD male patients by lncRNA UCA1 and aspirin
The aim of this study was to investigate the expression of long non-coding RNA urothelial carcinoma-associated 1 (UCA1) and its role in TNF-α production as one of the main inflammatory cytokines, in peripheral blood mononuclear cells (PBMCs) of patients with coronary artery disease (CAD) and healthy...
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| Format: | Article |
| Language: | English |
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University of Belgrade, University of Novi Sad
2020-01-01
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| Series: | Archives of Biological Sciences |
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| Online Access: | http://www.doiserbia.nb.rs/img/doi/0354-4664/2020/0354-46642000040N.pdf |
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| author | Nowrouzi-Sohrabi Peyman Seghatoleslam Atefeh Izadpanah Peyman Erfani Mehran Ahmadvand Hassan Kalani Mehdi |
| author_facet | Nowrouzi-Sohrabi Peyman Seghatoleslam Atefeh Izadpanah Peyman Erfani Mehran Ahmadvand Hassan Kalani Mehdi |
| author_sort | Nowrouzi-Sohrabi Peyman |
| collection | DOAJ |
| description | The aim of this study was to investigate the expression of long non-coding RNA urothelial carcinoma-associated 1 (UCA1) and its role in TNF-α production as one of the main inflammatory cytokines, in peripheral blood mononuclear cells (PBMCs) of patients with coronary artery disease (CAD) and healthy non-CAD (NCAD) individuals as the control group. Fifteen CAD and 15 NCAD individuals were enrolled in the study. UCA1 expression in PBMCs and the plasma concentrations of interleukin (IL)-6, IL-22 and tumor necrosis factor (TNF)-α were assessed by real-time PCR and flowcytometry, respectively. UCA1 expression was not significantly different between the CAD and NCAD groups, however, its level was higher in PBMCs of regular aspirin users of both study groups. Furthermore, aspirin users showed a significantly lower plasma level of TNF-α in comparison to non-aspirin users. In addition, UCA1 expression was negatively correlated with the level of TNF-α in the total sample of the examined population. It seems that the increased levels of UCA1 may be an underlying mechanism for downregulation of TNF-α in aspirin users. |
| first_indexed | 2024-12-17T05:57:07Z |
| format | Article |
| id | doaj.art-ff31ac66f2ea426d86f3fa0b223acf07 |
| institution | Directory Open Access Journal |
| issn | 0354-4664 1821-4339 |
| language | English |
| last_indexed | 2024-12-17T05:57:07Z |
| publishDate | 2020-01-01 |
| publisher | University of Belgrade, University of Novi Sad |
| record_format | Article |
| series | Archives of Biological Sciences |
| spelling | doaj.art-ff31ac66f2ea426d86f3fa0b223acf072022-12-21T22:00:59ZengUniversity of Belgrade, University of Novi SadArchives of Biological Sciences0354-46641821-43392020-01-0172446547110.2298/ABS200616040N0354-46642000040NModulation of TNF-α plasma levels in coronary artery disease [CAD] and non-CAD male patients by lncRNA UCA1 and aspirinNowrouzi-Sohrabi Peyman0Seghatoleslam Atefeh1Izadpanah Peyman2Erfani Mehran3Ahmadvand Hassan4Kalani Mehdi5Department of Biochemistry, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran + Student Research Committee, Shiraz University of Medical Sciences, Shiraz, IranDepartment of Biochemistry, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran + Research Center for Traditional Medicine and History of Medicine, Medical School, Shiraz University of Medical Sciences, Shiraz, IranCardiology Department, Shiraz University of Medical sciences, Shiraz, IranDepartment of Medical Laboratory Sciences, Faculty of Medical Sciences, Islamic Azad University, Arak Branch, Arak, IranDepartment of Biochemistry, Faculty of Medicine, Lorestan University of Medical Sciences, Khorramabad, Iran + Razi Herbal Researches Center, Lorestan University of Medical Sciences, Khorramabad, IranProfessor Alborzi Clinical Microbiology Research Center, Shiraz University of Medical Sciences, Shiraz, IranThe aim of this study was to investigate the expression of long non-coding RNA urothelial carcinoma-associated 1 (UCA1) and its role in TNF-α production as one of the main inflammatory cytokines, in peripheral blood mononuclear cells (PBMCs) of patients with coronary artery disease (CAD) and healthy non-CAD (NCAD) individuals as the control group. Fifteen CAD and 15 NCAD individuals were enrolled in the study. UCA1 expression in PBMCs and the plasma concentrations of interleukin (IL)-6, IL-22 and tumor necrosis factor (TNF)-α were assessed by real-time PCR and flowcytometry, respectively. UCA1 expression was not significantly different between the CAD and NCAD groups, however, its level was higher in PBMCs of regular aspirin users of both study groups. Furthermore, aspirin users showed a significantly lower plasma level of TNF-α in comparison to non-aspirin users. In addition, UCA1 expression was negatively correlated with the level of TNF-α in the total sample of the examined population. It seems that the increased levels of UCA1 may be an underlying mechanism for downregulation of TNF-α in aspirin users.http://www.doiserbia.nb.rs/img/doi/0354-4664/2020/0354-46642000040N.pdfaspirinnoncoding rnauca1tnf-αcoronary artery disease |
| spellingShingle | Nowrouzi-Sohrabi Peyman Seghatoleslam Atefeh Izadpanah Peyman Erfani Mehran Ahmadvand Hassan Kalani Mehdi Modulation of TNF-α plasma levels in coronary artery disease [CAD] and non-CAD male patients by lncRNA UCA1 and aspirin Archives of Biological Sciences aspirin noncoding rna uca1 tnf-α coronary artery disease |
| title | Modulation of TNF-α plasma levels in coronary artery disease [CAD] and non-CAD male patients by lncRNA UCA1 and aspirin |
| title_full | Modulation of TNF-α plasma levels in coronary artery disease [CAD] and non-CAD male patients by lncRNA UCA1 and aspirin |
| title_fullStr | Modulation of TNF-α plasma levels in coronary artery disease [CAD] and non-CAD male patients by lncRNA UCA1 and aspirin |
| title_full_unstemmed | Modulation of TNF-α plasma levels in coronary artery disease [CAD] and non-CAD male patients by lncRNA UCA1 and aspirin |
| title_short | Modulation of TNF-α plasma levels in coronary artery disease [CAD] and non-CAD male patients by lncRNA UCA1 and aspirin |
| title_sort | modulation of tnf α plasma levels in coronary artery disease cad and non cad male patients by lncrna uca1 and aspirin |
| topic | aspirin noncoding rna uca1 tnf-α coronary artery disease |
| url | http://www.doiserbia.nb.rs/img/doi/0354-4664/2020/0354-46642000040N.pdf |
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