Molecular evidence of early vertical transmission of Leishmania ( Leishmania ) infantum in a dog
ABSTRACT: Visceral leishmaniasis (VL) is caused by the protozoon Leishmania infantum . Transmission of this parasite to hosts occurs mainly through the bite of infected sand flies. However, alternative infection routes have been hypothesized, especially in areas where the biological vector is absen...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Universidade Federal de Santa Maria
|
| Series: | Ciência Rural |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782017000100553&lng=en&tlng=en |
| _version_ | 1811264739407298560 |
|---|---|
| author | Vinícius Vasconcelos Gomes de Oliveira Rafael Antonio Nascimento Ramos Carlos Alberto do Nascimento Ramos Neurisvan Ramos Guerra Frederico Celso Lyra Maia Leucio Câmara Alves Valdemiro Amaro da Silva Junior |
| author_facet | Vinícius Vasconcelos Gomes de Oliveira Rafael Antonio Nascimento Ramos Carlos Alberto do Nascimento Ramos Neurisvan Ramos Guerra Frederico Celso Lyra Maia Leucio Câmara Alves Valdemiro Amaro da Silva Junior |
| author_sort | Vinícius Vasconcelos Gomes de Oliveira |
| collection | DOAJ |
| description | ABSTRACT: Visceral leishmaniasis (VL) is caused by the protozoon Leishmania infantum . Transmission of this parasite to hosts occurs mainly through the bite of infected sand flies. However, alternative infection routes have been hypothesized, especially in areas where the biological vector is absent. The exact time of infection and whether in utero transmission occurs have still not been fully elucidated. This report demonstrates molecular evidence of vertical transmission of L. infantum from a pregnant dog to the embryo. Samples (e.g. vulva, vagina, cervix, uterine body, uterine horn and ovaries) from a female naturally infected by L. infantum and from her embryo were molecularly analyzed by means of qPCR and cPCR followed by DNA sequencing. The gestational age was estimated to be 23±1 day. Through qPCR, the presence of L. infantum DNA was detected in all the samples analyzed (n=7), including the embryo, conversely through cPCR, only four samples (vagina, cervix, uterine body and embryo) were positive. This study demonstrated that transmission of L. infantum from a pregnant dog to the embryo might occur in the early days of pregnancy. In conclusion, this is the first report showing L. infantum infecting a canine embryo. |
| first_indexed | 2024-04-12T20:09:48Z |
| format | Article |
| id | doaj.art-ff340a223fcb46719f04a0f5609c2a19 |
| institution | Directory Open Access Journal |
| issn | 1678-4596 |
| language | English |
| last_indexed | 2024-04-12T20:09:48Z |
| publisher | Universidade Federal de Santa Maria |
| record_format | Article |
| series | Ciência Rural |
| spelling | doaj.art-ff340a223fcb46719f04a0f5609c2a192022-12-22T03:18:18ZengUniversidade Federal de Santa MariaCiência Rural1678-459647110.1590/0103-8478cr20160553S0103-84782017000100553Molecular evidence of early vertical transmission of Leishmania ( Leishmania ) infantum in a dogVinícius Vasconcelos Gomes de OliveiraRafael Antonio Nascimento RamosCarlos Alberto do Nascimento RamosNeurisvan Ramos GuerraFrederico Celso Lyra MaiaLeucio Câmara AlvesValdemiro Amaro da Silva JuniorABSTRACT: Visceral leishmaniasis (VL) is caused by the protozoon Leishmania infantum . Transmission of this parasite to hosts occurs mainly through the bite of infected sand flies. However, alternative infection routes have been hypothesized, especially in areas where the biological vector is absent. The exact time of infection and whether in utero transmission occurs have still not been fully elucidated. This report demonstrates molecular evidence of vertical transmission of L. infantum from a pregnant dog to the embryo. Samples (e.g. vulva, vagina, cervix, uterine body, uterine horn and ovaries) from a female naturally infected by L. infantum and from her embryo were molecularly analyzed by means of qPCR and cPCR followed by DNA sequencing. The gestational age was estimated to be 23±1 day. Through qPCR, the presence of L. infantum DNA was detected in all the samples analyzed (n=7), including the embryo, conversely through cPCR, only four samples (vagina, cervix, uterine body and embryo) were positive. This study demonstrated that transmission of L. infantum from a pregnant dog to the embryo might occur in the early days of pregnancy. In conclusion, this is the first report showing L. infantum infecting a canine embryo.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782017000100553&lng=en&tlng=enleishmaniasisdogvertical infectionembryo |
| spellingShingle | Vinícius Vasconcelos Gomes de Oliveira Rafael Antonio Nascimento Ramos Carlos Alberto do Nascimento Ramos Neurisvan Ramos Guerra Frederico Celso Lyra Maia Leucio Câmara Alves Valdemiro Amaro da Silva Junior Molecular evidence of early vertical transmission of Leishmania ( Leishmania ) infantum in a dog Ciência Rural leishmaniasis dog vertical infection embryo |
| title | Molecular evidence of early vertical transmission of Leishmania ( Leishmania ) infantum in a dog |
| title_full | Molecular evidence of early vertical transmission of Leishmania ( Leishmania ) infantum in a dog |
| title_fullStr | Molecular evidence of early vertical transmission of Leishmania ( Leishmania ) infantum in a dog |
| title_full_unstemmed | Molecular evidence of early vertical transmission of Leishmania ( Leishmania ) infantum in a dog |
| title_short | Molecular evidence of early vertical transmission of Leishmania ( Leishmania ) infantum in a dog |
| title_sort | molecular evidence of early vertical transmission of leishmania leishmania infantum in a dog |
| topic | leishmaniasis dog vertical infection embryo |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782017000100553&lng=en&tlng=en |
| work_keys_str_mv | AT viniciusvasconcelosgomesdeoliveira molecularevidenceofearlyverticaltransmissionofleishmanialeishmaniainfantuminadog AT rafaelantonionascimentoramos molecularevidenceofearlyverticaltransmissionofleishmanialeishmaniainfantuminadog AT carlosalbertodonascimentoramos molecularevidenceofearlyverticaltransmissionofleishmanialeishmaniainfantuminadog AT neurisvanramosguerra molecularevidenceofearlyverticaltransmissionofleishmanialeishmaniainfantuminadog AT fredericocelsolyramaia molecularevidenceofearlyverticaltransmissionofleishmanialeishmaniainfantuminadog AT leuciocamaraalves molecularevidenceofearlyverticaltransmissionofleishmanialeishmaniainfantuminadog AT valdemiroamarodasilvajunior molecularevidenceofearlyverticaltransmissionofleishmanialeishmaniainfantuminadog |