Using a micro-physiological system to prolong the preservation of ex vivo lung tissue
Current in vitro and in vivo disease models have been reported to lack sufficient translation to human. Precision-Cut Lung Slices (PCLS) are viable sections of lung tissue and have been described to be a translational model for the ex vivo assessment of pharmacological and toxicological compounds. I...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
De Gruyter
2021-10-01
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| Series: | Current Directions in Biomedical Engineering |
| Subjects: | |
| Online Access: | https://doi.org/10.1515/cdbme-2021-2053 |
| _version_ | 1811194193435951104 |
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| author | Böhlen Sebastian Konzok Sebastian Labisch Jennifer Dehmel Susann Schaudien Dirk Behrens Stephan Schmieder Florian Braun Armin Sonntag Frank Sewald Katherina |
| author_facet | Böhlen Sebastian Konzok Sebastian Labisch Jennifer Dehmel Susann Schaudien Dirk Behrens Stephan Schmieder Florian Braun Armin Sonntag Frank Sewald Katherina |
| author_sort | Böhlen Sebastian |
| collection | DOAJ |
| description | Current in vitro and in vivo disease models have been reported to lack sufficient translation to human. Precision-Cut Lung Slices (PCLS) are viable sections of lung tissue and have been described to be a translational model for the ex vivo assessment of pharmacological and toxicological compounds. In most studies PCLS were cultured under static conditions. These lung sections, however, suffer from the limited viability. Here we present a novel modular microphysiological system (MPS) to prolong the cultivation of ex vivo lung tissue. A tailored MPS setup was designed using the PDMS free modular plug&play MPS construction kit. PCLS from mice were cultivated for up to one week under static versus perfused conditions. Using the MPS technology enabled a prolonged culture period with improved viability as shown by lowered lactate dehydrogenase release and improved membrane integrity. Using this technology might allow us to use PCLS for longer culture periods such as e.g. repeated dose toxicity or pharmacology studies. |
| first_indexed | 2024-04-12T00:22:56Z |
| format | Article |
| id | doaj.art-ff367e594df644449ad4974e90589a88 |
| institution | Directory Open Access Journal |
| issn | 2364-5504 |
| language | English |
| last_indexed | 2024-04-12T00:22:56Z |
| publishDate | 2021-10-01 |
| publisher | De Gruyter |
| record_format | Article |
| series | Current Directions in Biomedical Engineering |
| spelling | doaj.art-ff367e594df644449ad4974e90589a882022-12-22T03:55:41ZengDe GruyterCurrent Directions in Biomedical Engineering2364-55042021-10-017220721010.1515/cdbme-2021-2053Using a micro-physiological system to prolong the preservation of ex vivo lung tissueBöhlen Sebastian0Konzok Sebastian1Labisch Jennifer2Dehmel Susann3Schaudien Dirk4Behrens Stephan5Schmieder Florian6Braun Armin7Sonntag Frank8Sewald Katherina9Fraunhofer ITEM, Hannover, Germany, Member of the German Center for Lung research (DZL), Biomedical Research in Endstage and Obstructive Lung DiseaseHannover(Breath), GermanyFraunhofer ITEM, Hannover, Germany, Member of the German Center for Lung research (DZL), Biomedical Research in Endstage and Obstructive Lung DiseaseHannover(Breath), GermanyFraunhofer ITEM, Hannover, Germany, Member of the German Center for Lung research (DZL), Biomedical Research in Endstage and Obstructive Lung DiseaseHannover(Breath), GermanyFraunhofer ITEM, Hannover, Germany, Member of the German Center for Lung research (DZL), Biomedical Research in Endstage and Obstructive Lung DiseaseHannover(Breath), GermanyFraunhofer ITEM, Hannover, Germany, Member of the German Center for Lung research (DZL), Biomedical Research in Endstage and Obstructive Lung DiseaseHannover(Breath), GermanyFraunhofer Institute for Material and Beam Technology (IWS),Dresden, GermanyFraunhofer Institute for Material and Beam Technology (IWS),Dresden, GermanyFraunhofer ITEM, Hannover, Germany, Member of the German Center for Lung research (DZL), Biomedical Research in Endstage and Obstructive Lung DiseaseHannover(Breath), GermanyFraunhofer Institute for Material and Beam Technology (IWS),Dresden, GermanyFraunhofer ITEM, Nikolai-Fuchs-Straße 1,Hannover, GermanyCurrent in vitro and in vivo disease models have been reported to lack sufficient translation to human. Precision-Cut Lung Slices (PCLS) are viable sections of lung tissue and have been described to be a translational model for the ex vivo assessment of pharmacological and toxicological compounds. In most studies PCLS were cultured under static conditions. These lung sections, however, suffer from the limited viability. Here we present a novel modular microphysiological system (MPS) to prolong the cultivation of ex vivo lung tissue. A tailored MPS setup was designed using the PDMS free modular plug&play MPS construction kit. PCLS from mice were cultivated for up to one week under static versus perfused conditions. Using the MPS technology enabled a prolonged culture period with improved viability as shown by lowered lactate dehydrogenase release and improved membrane integrity. Using this technology might allow us to use PCLS for longer culture periods such as e.g. repeated dose toxicity or pharmacology studies.https://doi.org/10.1515/cdbme-2021-2053precision-cut lung slicesorgan-on-a-chiprespiratory disease modelslong-term cultivation |
| spellingShingle | Böhlen Sebastian Konzok Sebastian Labisch Jennifer Dehmel Susann Schaudien Dirk Behrens Stephan Schmieder Florian Braun Armin Sonntag Frank Sewald Katherina Using a micro-physiological system to prolong the preservation of ex vivo lung tissue Current Directions in Biomedical Engineering precision-cut lung slices organ-on-a-chip respiratory disease models long-term cultivation |
| title | Using a micro-physiological system to prolong the preservation of ex vivo lung tissue |
| title_full | Using a micro-physiological system to prolong the preservation of ex vivo lung tissue |
| title_fullStr | Using a micro-physiological system to prolong the preservation of ex vivo lung tissue |
| title_full_unstemmed | Using a micro-physiological system to prolong the preservation of ex vivo lung tissue |
| title_short | Using a micro-physiological system to prolong the preservation of ex vivo lung tissue |
| title_sort | using a micro physiological system to prolong the preservation of ex vivo lung tissue |
| topic | precision-cut lung slices organ-on-a-chip respiratory disease models long-term cultivation |
| url | https://doi.org/10.1515/cdbme-2021-2053 |
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