The Sensitivity of Endodontic Enterococcus spp. Strains to Geranium Essential Oil
Enterococci are able to survive endodontic procedures and contribute to the failure of endodontic therapy. Thus, it is essential to identify novel ways of eradicating them from infected root canals. One such approach may be the use of antimicrobials such as plant essential oils. Enterococcal strains...
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| Format: | Article |
| Language: | English |
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MDPI AG
2015-12-01
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| Series: | Molecules |
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| Online Access: | http://www.mdpi.com/1420-3049/20/12/19888 |
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| author | Monika E. Łysakowska Monika Sienkiewicz Katarzyna Banaszek Jerzy Sokołowski |
| author_facet | Monika E. Łysakowska Monika Sienkiewicz Katarzyna Banaszek Jerzy Sokołowski |
| author_sort | Monika E. Łysakowska |
| collection | DOAJ |
| description | Enterococci are able to survive endodontic procedures and contribute to the failure of endodontic therapy. Thus, it is essential to identify novel ways of eradicating them from infected root canals. One such approach may be the use of antimicrobials such as plant essential oils. Enterococcal strains were isolated from endodontically treated teeth by standard microbiological methods. Susceptibility to antibiotics was evaluated by the disc-diffusion method. The minimal inhibitory concentration (MIC) of geranium essential oil was investigated by microdilution in 96-well microplates in Mueller Hinton Broth II. Biofilm eradication concentrations were checked in dentin tests. Geranium essential oil inhibited enterococcal strains at concentrations ranging from 1.8–4.5 mg/mL. No correlation was shown between resistance to antibiotics and the MICs of the test antimicrobials. The MICs of the test oil were lower than those found to show cytotoxic effects on the HMEC-1 cell line. Geranium essential oil eradicated enterococcal biofilm at concentrations of 150 mg/mL. Geranium essential oil inhibits the growth of endodontic enterococcal species at lower concentrations than those required to reach IC50 against the HMEC-1 cell line, and is effective against bacteria protected in biofilm at higher concentrations. In addition, bacteria do not develop resistance to essential oils. Hence, geranium essential oil represents a possible alternative to other antimicrobials during endodontic procedures. |
| first_indexed | 2024-04-12T08:27:04Z |
| format | Article |
| id | doaj.art-ff3750bb6f2f478c81aec89daba3e213 |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-04-12T08:27:04Z |
| publishDate | 2015-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-ff3750bb6f2f478c81aec89daba3e2132022-12-22T03:40:21ZengMDPI AGMolecules1420-30492015-12-012012228812288910.3390/molecules201219888molecules201219888The Sensitivity of Endodontic Enterococcus spp. Strains to Geranium Essential OilMonika E. Łysakowska0Monika Sienkiewicz1Katarzyna Banaszek2Jerzy Sokołowski3Department of Microbiology and Medical Laboratory Immunology, Medical University of Lodz, 92-231 Lodz, Pomorska 251, PolandDepartment of Environmental Biology, Medical University of Lodz, 90-752 Lodz, Żeligowskiego 7/9, PolandDepartment of General Dentistry, Medical University of Lodz, 92-231 Lodz, Pomorska 251, PolandDepartment of General Dentistry, Medical University of Lodz, 92-231 Lodz, Pomorska 251, PolandEnterococci are able to survive endodontic procedures and contribute to the failure of endodontic therapy. Thus, it is essential to identify novel ways of eradicating them from infected root canals. One such approach may be the use of antimicrobials such as plant essential oils. Enterococcal strains were isolated from endodontically treated teeth by standard microbiological methods. Susceptibility to antibiotics was evaluated by the disc-diffusion method. The minimal inhibitory concentration (MIC) of geranium essential oil was investigated by microdilution in 96-well microplates in Mueller Hinton Broth II. Biofilm eradication concentrations were checked in dentin tests. Geranium essential oil inhibited enterococcal strains at concentrations ranging from 1.8–4.5 mg/mL. No correlation was shown between resistance to antibiotics and the MICs of the test antimicrobials. The MICs of the test oil were lower than those found to show cytotoxic effects on the HMEC-1 cell line. Geranium essential oil eradicated enterococcal biofilm at concentrations of 150 mg/mL. Geranium essential oil inhibits the growth of endodontic enterococcal species at lower concentrations than those required to reach IC50 against the HMEC-1 cell line, and is effective against bacteria protected in biofilm at higher concentrations. In addition, bacteria do not develop resistance to essential oils. Hence, geranium essential oil represents a possible alternative to other antimicrobials during endodontic procedures.http://www.mdpi.com/1420-3049/20/12/19888enterococciendodontic treatmentgeranium essential oil |
| spellingShingle | Monika E. Łysakowska Monika Sienkiewicz Katarzyna Banaszek Jerzy Sokołowski The Sensitivity of Endodontic Enterococcus spp. Strains to Geranium Essential Oil Molecules enterococci endodontic treatment geranium essential oil |
| title | The Sensitivity of Endodontic Enterococcus spp. Strains to Geranium Essential Oil |
| title_full | The Sensitivity of Endodontic Enterococcus spp. Strains to Geranium Essential Oil |
| title_fullStr | The Sensitivity of Endodontic Enterococcus spp. Strains to Geranium Essential Oil |
| title_full_unstemmed | The Sensitivity of Endodontic Enterococcus spp. Strains to Geranium Essential Oil |
| title_short | The Sensitivity of Endodontic Enterococcus spp. Strains to Geranium Essential Oil |
| title_sort | sensitivity of endodontic enterococcus spp strains to geranium essential oil |
| topic | enterococci endodontic treatment geranium essential oil |
| url | http://www.mdpi.com/1420-3049/20/12/19888 |
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