Impact of maximal exercise on immune cell mobilization and bioenergetics
Abstract Acute aerobic exercise increases the number and proportions of circulating peripheral blood mononuclear cells (PMBC) and can alter PBMC mitochondrial bioenergetics. In this study, we aimed to examine the impact of a maximal exercise bout on immune cell metabolism in collegiate swimmers. Ele...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2023-06-01
|
| Series: | Physiological Reports |
| Subjects: | |
| Online Access: | https://doi.org/10.14814/phy2.15753 |
| _version_ | 1827589745656463360 |
|---|---|
| author | James E. Stampley Eunhan Cho Haoyan Wang Bailey Theall Neil M. Johannsen Guillaume Spielmann Brian A. Irving |
| author_facet | James E. Stampley Eunhan Cho Haoyan Wang Bailey Theall Neil M. Johannsen Guillaume Spielmann Brian A. Irving |
| author_sort | James E. Stampley |
| collection | DOAJ |
| description | Abstract Acute aerobic exercise increases the number and proportions of circulating peripheral blood mononuclear cells (PMBC) and can alter PBMC mitochondrial bioenergetics. In this study, we aimed to examine the impact of a maximal exercise bout on immune cell metabolism in collegiate swimmers. Eleven (7 M/4F) collegiate swimmers completed a maximal exercise test to measure anaerobic power and capacity. Pre‐ and postexercise PBMCs were isolated to measure the immune cell phenotypes and mitochondrial bioenergetics using flow cytometry and high‐resolution respirometry. The maximal exercise bout increased circulating levels of PBMCs, particularly in central memory (KLRG1+/CD57−) and senescent (KLRG1+/CD57+) CD8+ T cells, whether measured as a % of PMBCs or as absolute concentrations (all p < 0.05). At the cellularlevel, the routine oxygen flow (IO2 [pmol·s−1·106 PBMCs−1]) increased following maximal exercise (p = 0.042); however, there were no effects of exercise on the IO2 measured under the LEAK, oxidative phosphorylation (OXPHOS), or electron transfer (ET) capacities. There were exercise‐induced increases in the tissue‐level oxygen flow (IO2‐tissue [pmol·s−1·mL blood−1]) for all respiratory states (all p < 0.01), except for the LEAK state, after accounting for the mobilization of PBMCs. Future subtype‐specific studies are needed to characterize further maximal exercise's true impact on immune cell bioenergetics. |
| first_indexed | 2024-03-09T01:06:16Z |
| format | Article |
| id | doaj.art-ff397b75a3ff4e9c8456745c221cd00f |
| institution | Directory Open Access Journal |
| issn | 2051-817X |
| language | English |
| last_indexed | 2024-03-09T01:06:16Z |
| publishDate | 2023-06-01 |
| publisher | Wiley |
| record_format | Article |
| series | Physiological Reports |
| spelling | doaj.art-ff397b75a3ff4e9c8456745c221cd00f2023-12-11T09:37:13ZengWileyPhysiological Reports2051-817X2023-06-011111n/an/a10.14814/phy2.15753Impact of maximal exercise on immune cell mobilization and bioenergeticsJames E. Stampley0Eunhan Cho1Haoyan Wang2Bailey Theall3Neil M. Johannsen4Guillaume Spielmann5Brian A. Irving6School of Kinesiology Louisiana State University Baton Rouge Louisiana USASchool of Kinesiology Louisiana State University Baton Rouge Louisiana USASchool of Kinesiology Louisiana State University Baton Rouge Louisiana USASchool of Kinesiology Louisiana State University Baton Rouge Louisiana USASchool of Kinesiology Louisiana State University Baton Rouge Louisiana USASchool of Kinesiology Louisiana State University Baton Rouge Louisiana USASchool of Kinesiology Louisiana State University Baton Rouge Louisiana USAAbstract Acute aerobic exercise increases the number and proportions of circulating peripheral blood mononuclear cells (PMBC) and can alter PBMC mitochondrial bioenergetics. In this study, we aimed to examine the impact of a maximal exercise bout on immune cell metabolism in collegiate swimmers. Eleven (7 M/4F) collegiate swimmers completed a maximal exercise test to measure anaerobic power and capacity. Pre‐ and postexercise PBMCs were isolated to measure the immune cell phenotypes and mitochondrial bioenergetics using flow cytometry and high‐resolution respirometry. The maximal exercise bout increased circulating levels of PBMCs, particularly in central memory (KLRG1+/CD57−) and senescent (KLRG1+/CD57+) CD8+ T cells, whether measured as a % of PMBCs or as absolute concentrations (all p < 0.05). At the cellularlevel, the routine oxygen flow (IO2 [pmol·s−1·106 PBMCs−1]) increased following maximal exercise (p = 0.042); however, there were no effects of exercise on the IO2 measured under the LEAK, oxidative phosphorylation (OXPHOS), or electron transfer (ET) capacities. There were exercise‐induced increases in the tissue‐level oxygen flow (IO2‐tissue [pmol·s−1·mL blood−1]) for all respiratory states (all p < 0.01), except for the LEAK state, after accounting for the mobilization of PBMCs. Future subtype‐specific studies are needed to characterize further maximal exercise's true impact on immune cell bioenergetics.https://doi.org/10.14814/phy2.15753athletesinflammationmitochondriaoxygraphPBMCrespirometry |
| spellingShingle | James E. Stampley Eunhan Cho Haoyan Wang Bailey Theall Neil M. Johannsen Guillaume Spielmann Brian A. Irving Impact of maximal exercise on immune cell mobilization and bioenergetics Physiological Reports athletes inflammation mitochondria oxygraph PBMC respirometry |
| title | Impact of maximal exercise on immune cell mobilization and bioenergetics |
| title_full | Impact of maximal exercise on immune cell mobilization and bioenergetics |
| title_fullStr | Impact of maximal exercise on immune cell mobilization and bioenergetics |
| title_full_unstemmed | Impact of maximal exercise on immune cell mobilization and bioenergetics |
| title_short | Impact of maximal exercise on immune cell mobilization and bioenergetics |
| title_sort | impact of maximal exercise on immune cell mobilization and bioenergetics |
| topic | athletes inflammation mitochondria oxygraph PBMC respirometry |
| url | https://doi.org/10.14814/phy2.15753 |
| work_keys_str_mv | AT jamesestampley impactofmaximalexerciseonimmunecellmobilizationandbioenergetics AT eunhancho impactofmaximalexerciseonimmunecellmobilizationandbioenergetics AT haoyanwang impactofmaximalexerciseonimmunecellmobilizationandbioenergetics AT baileytheall impactofmaximalexerciseonimmunecellmobilizationandbioenergetics AT neilmjohannsen impactofmaximalexerciseonimmunecellmobilizationandbioenergetics AT guillaumespielmann impactofmaximalexerciseonimmunecellmobilizationandbioenergetics AT brianairving impactofmaximalexerciseonimmunecellmobilizationandbioenergetics |