PP1-Dependent Formin Bnr1 Dephosphorylation and Delocalization from a Cell Division Site.
Cell cycle ends with cytokinesis that is the physical separation of a cell into two daughter cells. For faithful cytokinesis, cells integrate multiple processes, such as actomyosin ring formation, contraction and plasma membrane closure, into coherent responses. Linear actin assembly by formins is e...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2016-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC4714816?pdf=render |
| _version_ | 1811262701251330048 |
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| author | Minami Orii Keiko Kono Hsin-I Wen Makoto Nakanishi |
| author_facet | Minami Orii Keiko Kono Hsin-I Wen Makoto Nakanishi |
| author_sort | Minami Orii |
| collection | DOAJ |
| description | Cell cycle ends with cytokinesis that is the physical separation of a cell into two daughter cells. For faithful cytokinesis, cells integrate multiple processes, such as actomyosin ring formation, contraction and plasma membrane closure, into coherent responses. Linear actin assembly by formins is essential for formation and maintenance of actomyosin ring. Although budding yeast's two formins, Bni1 and Bnr1, are known to switch their subcellular localization at the division site prior to cytokinesis, the underlying mechanisms were not completely understood. Here, we provide evidence showing that Bnr1 is dephosphorylated concomitant with its release from the division site. Impaired PP1/Glc7 activity delayed Bnr1 release and dephosphorylation, Bni1 recruitment and actomyosin ring formation at the division site. These results suggest the involvement of Glc7 in this regulation. Further, we identified Ref2 as the PP1 regulatory subunit responsible for this regulation. Taken together, Glc7 and Ref2 may have a role in actomyosin ring formation by modulating the localization of formins during cytokinesis. |
| first_indexed | 2024-04-12T19:31:25Z |
| format | Article |
| id | doaj.art-ff39d9d39d524b8e99ea50b4f631843b |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-04-12T19:31:25Z |
| publishDate | 2016-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-ff39d9d39d524b8e99ea50b4f631843b2022-12-22T03:19:20ZengPublic Library of Science (PLoS)PLoS ONE1932-62032016-01-01111e014694110.1371/journal.pone.0146941PP1-Dependent Formin Bnr1 Dephosphorylation and Delocalization from a Cell Division Site.Minami OriiKeiko KonoHsin-I WenMakoto NakanishiCell cycle ends with cytokinesis that is the physical separation of a cell into two daughter cells. For faithful cytokinesis, cells integrate multiple processes, such as actomyosin ring formation, contraction and plasma membrane closure, into coherent responses. Linear actin assembly by formins is essential for formation and maintenance of actomyosin ring. Although budding yeast's two formins, Bni1 and Bnr1, are known to switch their subcellular localization at the division site prior to cytokinesis, the underlying mechanisms were not completely understood. Here, we provide evidence showing that Bnr1 is dephosphorylated concomitant with its release from the division site. Impaired PP1/Glc7 activity delayed Bnr1 release and dephosphorylation, Bni1 recruitment and actomyosin ring formation at the division site. These results suggest the involvement of Glc7 in this regulation. Further, we identified Ref2 as the PP1 regulatory subunit responsible for this regulation. Taken together, Glc7 and Ref2 may have a role in actomyosin ring formation by modulating the localization of formins during cytokinesis.http://europepmc.org/articles/PMC4714816?pdf=render |
| spellingShingle | Minami Orii Keiko Kono Hsin-I Wen Makoto Nakanishi PP1-Dependent Formin Bnr1 Dephosphorylation and Delocalization from a Cell Division Site. PLoS ONE |
| title | PP1-Dependent Formin Bnr1 Dephosphorylation and Delocalization from a Cell Division Site. |
| title_full | PP1-Dependent Formin Bnr1 Dephosphorylation and Delocalization from a Cell Division Site. |
| title_fullStr | PP1-Dependent Formin Bnr1 Dephosphorylation and Delocalization from a Cell Division Site. |
| title_full_unstemmed | PP1-Dependent Formin Bnr1 Dephosphorylation and Delocalization from a Cell Division Site. |
| title_short | PP1-Dependent Formin Bnr1 Dephosphorylation and Delocalization from a Cell Division Site. |
| title_sort | pp1 dependent formin bnr1 dephosphorylation and delocalization from a cell division site |
| url | http://europepmc.org/articles/PMC4714816?pdf=render |
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