The CRK5 and WRKY53 Are Conditional Regulators of Senescence and Stomatal Conductance in <i>Arabidopsis</i>
In <i>Arabidopsis thaliana</i>, cysteine-rich receptor-like kinases (CRKs) constitute a large group of membrane-localized proteins which perceive external stimuli and transduce the signal into the cell. Previous reports based on their loss-of-function phenotypes and expression profile su...
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MDPI AG
2022-11-01
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| author | Paweł Burdiak Jakub Mielecki Piotr Gawroński Stanisław Karpiński |
| author_facet | Paweł Burdiak Jakub Mielecki Piotr Gawroński Stanisław Karpiński |
| author_sort | Paweł Burdiak |
| collection | DOAJ |
| description | In <i>Arabidopsis thaliana</i>, cysteine-rich receptor-like kinases (CRKs) constitute a large group of membrane-localized proteins which perceive external stimuli and transduce the signal into the cell. Previous reports based on their loss-of-function phenotypes and expression profile support their role in many developmental and stress-responsive pathways. Our study revealed that one member of this family, CRK5, acts as a negative regulator of leaf aging. Enrichment of the <i>CRK5</i> promoter region in W-box <i>cis</i>-elements demonstrated that WRKY transcription factors control it. We observed significantly enhanced <i>WRKY53</i> expression in <i>crk5</i> and reversion of its early-senescence phenotype in the <i>crk5 wrky53</i> line, suggesting a negative feedback loop between these proteins antagonistically regulating chlorophyll <i>a</i> and <i>b</i> contents. Yeast-two hybrid assay showed further that CRK5 interacts with several proteins involved in response to water deprivation or calcium signaling, while gas exchange analysis revealed a positive effect of CRK5 on water use efficiency. Consistent with that, the <i>crk5</i> plants showed disturbed foliar temperature, stomatal conductance, transpiration, and increased susceptibility to osmotic stress. These traits were fully or partially reverted to wild-type phenotype in <i>crk5 wrky53</i> double mutant. Obtained results suggest that WRKY53 and CRK5 are antagonistic regulators of chlorophyll synthesis/degradation, senescence, and stomatal conductance. |
| first_indexed | 2024-03-09T18:25:59Z |
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| id | doaj.art-ff3be5362c484bd7ac1616c252112a29 |
| institution | Directory Open Access Journal |
| issn | 2073-4409 |
| language | English |
| last_indexed | 2024-03-09T18:25:59Z |
| publishDate | 2022-11-01 |
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| series | Cells |
| spelling | doaj.art-ff3be5362c484bd7ac1616c252112a292023-11-24T07:57:23ZengMDPI AGCells2073-44092022-11-011122355810.3390/cells11223558The CRK5 and WRKY53 Are Conditional Regulators of Senescence and Stomatal Conductance in <i>Arabidopsis</i>Paweł Burdiak0Jakub Mielecki1Piotr Gawroński2Stanisław Karpiński3Department of Plant Genetics, Breeding, and Biotechnology, Institute of Biology, Warsaw University of Life Sciences, 02-776 Warsaw, PolandDepartment of Plant Genetics, Breeding, and Biotechnology, Institute of Biology, Warsaw University of Life Sciences, 02-776 Warsaw, PolandDepartment of Plant Genetics, Breeding, and Biotechnology, Institute of Biology, Warsaw University of Life Sciences, 02-776 Warsaw, PolandDepartment of Plant Genetics, Breeding, and Biotechnology, Institute of Biology, Warsaw University of Life Sciences, 02-776 Warsaw, PolandIn <i>Arabidopsis thaliana</i>, cysteine-rich receptor-like kinases (CRKs) constitute a large group of membrane-localized proteins which perceive external stimuli and transduce the signal into the cell. Previous reports based on their loss-of-function phenotypes and expression profile support their role in many developmental and stress-responsive pathways. Our study revealed that one member of this family, CRK5, acts as a negative regulator of leaf aging. Enrichment of the <i>CRK5</i> promoter region in W-box <i>cis</i>-elements demonstrated that WRKY transcription factors control it. We observed significantly enhanced <i>WRKY53</i> expression in <i>crk5</i> and reversion of its early-senescence phenotype in the <i>crk5 wrky53</i> line, suggesting a negative feedback loop between these proteins antagonistically regulating chlorophyll <i>a</i> and <i>b</i> contents. Yeast-two hybrid assay showed further that CRK5 interacts with several proteins involved in response to water deprivation or calcium signaling, while gas exchange analysis revealed a positive effect of CRK5 on water use efficiency. Consistent with that, the <i>crk5</i> plants showed disturbed foliar temperature, stomatal conductance, transpiration, and increased susceptibility to osmotic stress. These traits were fully or partially reverted to wild-type phenotype in <i>crk5 wrky53</i> double mutant. Obtained results suggest that WRKY53 and CRK5 are antagonistic regulators of chlorophyll synthesis/degradation, senescence, and stomatal conductance.https://www.mdpi.com/2073-4409/11/22/3558senescencereceptor-like kinasetranscription factorwater-use efficiencystomatal conductancefoliar temperature |
| spellingShingle | Paweł Burdiak Jakub Mielecki Piotr Gawroński Stanisław Karpiński The CRK5 and WRKY53 Are Conditional Regulators of Senescence and Stomatal Conductance in <i>Arabidopsis</i> Cells senescence receptor-like kinase transcription factor water-use efficiency stomatal conductance foliar temperature |
| title | The CRK5 and WRKY53 Are Conditional Regulators of Senescence and Stomatal Conductance in <i>Arabidopsis</i> |
| title_full | The CRK5 and WRKY53 Are Conditional Regulators of Senescence and Stomatal Conductance in <i>Arabidopsis</i> |
| title_fullStr | The CRK5 and WRKY53 Are Conditional Regulators of Senescence and Stomatal Conductance in <i>Arabidopsis</i> |
| title_full_unstemmed | The CRK5 and WRKY53 Are Conditional Regulators of Senescence and Stomatal Conductance in <i>Arabidopsis</i> |
| title_short | The CRK5 and WRKY53 Are Conditional Regulators of Senescence and Stomatal Conductance in <i>Arabidopsis</i> |
| title_sort | crk5 and wrky53 are conditional regulators of senescence and stomatal conductance in i arabidopsis i |
| topic | senescence receptor-like kinase transcription factor water-use efficiency stomatal conductance foliar temperature |
| url | https://www.mdpi.com/2073-4409/11/22/3558 |
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