Enhanced Muscle Fibers of <i>Epinephelus coioides</i> by <i>Myostatin</i> Autologous Nucleic Acid Vaccine
<i>Epinephelus coioides</i> is a fish species with high economic value due to its delicious meat, high protein content, and rich fatty acid nutrition. It has become a high-economic fish in southern parts of China and some other Southeast Asian countries. In this study, the <i>myost...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2022-06-01
|
| Series: | International Journal of Molecular Sciences |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1422-0067/23/13/6997 |
| _version_ | 1797479898285080576 |
|---|---|
| author | Bing Fu Jinzeng Yang Yan Yang Jun Xia Yinglin He Qing Wang Huihong Zhao Huirong Yang |
| author_facet | Bing Fu Jinzeng Yang Yan Yang Jun Xia Yinglin He Qing Wang Huihong Zhao Huirong Yang |
| author_sort | Bing Fu |
| collection | DOAJ |
| description | <i>Epinephelus coioides</i> is a fish species with high economic value due to its delicious meat, high protein content, and rich fatty acid nutrition. It has become a high-economic fish in southern parts of China and some other Southeast Asian countries. In this study, the <i>myostatin</i> nucleic acid vaccine was constructed and used to immunize <i>E. coioides</i>. The results from body length and weight measurements indicated the <i>myostatin</i> nucleic acid vaccine promoted <i>E. coioides</i> growth performance by increasing muscle fiber size. The results from RT-qPCR analysis showed that <i>myostatin</i> nucleic acid vaccine upregulated the expression of <i>myod</i>, <i>myog</i> and <i>p21</i> mRNA, downregulated the expression of <i>smad3</i> and <i>mrf4</i> mRNA. This preliminary study is the first report that explored the role of <i>myostatin</i> in <i>E. coioides</i> and showed positive effects of autologous nucleic acid vaccine on the muscle growth of <i>E. coioides</i>. Further experiments with increased numbers of animals and different doses are needed for its application to <i>E. coiodes</i> aquaculture production. |
| first_indexed | 2024-03-09T21:52:24Z |
| format | Article |
| id | doaj.art-ff414ee775c44829859e25b78046e19e |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-09T21:52:24Z |
| publishDate | 2022-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-ff414ee775c44829859e25b78046e19e2023-11-23T20:06:06ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672022-06-012313699710.3390/ijms23136997Enhanced Muscle Fibers of <i>Epinephelus coioides</i> by <i>Myostatin</i> Autologous Nucleic Acid VaccineBing Fu0Jinzeng Yang1Yan Yang2Jun Xia3Yinglin He4Qing Wang5Huihong Zhao6Huirong Yang7College of Marine Sciences, South China Agricultural University, Guangzhou 510640, ChinaDepartment of Human Nutrition, Food and Animal Sciences, University of Hawaii at Manoa, Honolulu, HI 96822, USACollege of Marine Sciences, South China Agricultural University, Guangzhou 510640, ChinaXinjiang Acadamy of Animal Sciences, Institute of Veterinary Medicine (Research Center of Animal Clinical), Urumqi 830000, ChinaCollege of Marine Sciences, South China Agricultural University, Guangzhou 510640, ChinaCollege of Marine Sciences, South China Agricultural University, Guangzhou 510640, ChinaCollege of Marine Sciences, South China Agricultural University, Guangzhou 510640, ChinaCollege of Marine Sciences, South China Agricultural University, Guangzhou 510640, China<i>Epinephelus coioides</i> is a fish species with high economic value due to its delicious meat, high protein content, and rich fatty acid nutrition. It has become a high-economic fish in southern parts of China and some other Southeast Asian countries. In this study, the <i>myostatin</i> nucleic acid vaccine was constructed and used to immunize <i>E. coioides</i>. The results from body length and weight measurements indicated the <i>myostatin</i> nucleic acid vaccine promoted <i>E. coioides</i> growth performance by increasing muscle fiber size. The results from RT-qPCR analysis showed that <i>myostatin</i> nucleic acid vaccine upregulated the expression of <i>myod</i>, <i>myog</i> and <i>p21</i> mRNA, downregulated the expression of <i>smad3</i> and <i>mrf4</i> mRNA. This preliminary study is the first report that explored the role of <i>myostatin</i> in <i>E. coioides</i> and showed positive effects of autologous nucleic acid vaccine on the muscle growth of <i>E. coioides</i>. Further experiments with increased numbers of animals and different doses are needed for its application to <i>E. coiodes</i> aquaculture production.https://www.mdpi.com/1422-0067/23/13/6997<i>Epinephelus coioides</i><i>myostatin</i>nucleic acid vaccineskeletal muscle |
| spellingShingle | Bing Fu Jinzeng Yang Yan Yang Jun Xia Yinglin He Qing Wang Huihong Zhao Huirong Yang Enhanced Muscle Fibers of <i>Epinephelus coioides</i> by <i>Myostatin</i> Autologous Nucleic Acid Vaccine International Journal of Molecular Sciences <i>Epinephelus coioides</i> <i>myostatin</i> nucleic acid vaccine skeletal muscle |
| title | Enhanced Muscle Fibers of <i>Epinephelus coioides</i> by <i>Myostatin</i> Autologous Nucleic Acid Vaccine |
| title_full | Enhanced Muscle Fibers of <i>Epinephelus coioides</i> by <i>Myostatin</i> Autologous Nucleic Acid Vaccine |
| title_fullStr | Enhanced Muscle Fibers of <i>Epinephelus coioides</i> by <i>Myostatin</i> Autologous Nucleic Acid Vaccine |
| title_full_unstemmed | Enhanced Muscle Fibers of <i>Epinephelus coioides</i> by <i>Myostatin</i> Autologous Nucleic Acid Vaccine |
| title_short | Enhanced Muscle Fibers of <i>Epinephelus coioides</i> by <i>Myostatin</i> Autologous Nucleic Acid Vaccine |
| title_sort | enhanced muscle fibers of i epinephelus coioides i by i myostatin i autologous nucleic acid vaccine |
| topic | <i>Epinephelus coioides</i> <i>myostatin</i> nucleic acid vaccine skeletal muscle |
| url | https://www.mdpi.com/1422-0067/23/13/6997 |
| work_keys_str_mv | AT bingfu enhancedmusclefibersofiepinepheluscoioidesibyimyostatiniautologousnucleicacidvaccine AT jinzengyang enhancedmusclefibersofiepinepheluscoioidesibyimyostatiniautologousnucleicacidvaccine AT yanyang enhancedmusclefibersofiepinepheluscoioidesibyimyostatiniautologousnucleicacidvaccine AT junxia enhancedmusclefibersofiepinepheluscoioidesibyimyostatiniautologousnucleicacidvaccine AT yinglinhe enhancedmusclefibersofiepinepheluscoioidesibyimyostatiniautologousnucleicacidvaccine AT qingwang enhancedmusclefibersofiepinepheluscoioidesibyimyostatiniautologousnucleicacidvaccine AT huihongzhao enhancedmusclefibersofiepinepheluscoioidesibyimyostatiniautologousnucleicacidvaccine AT huirongyang enhancedmusclefibersofiepinepheluscoioidesibyimyostatiniautologousnucleicacidvaccine |