Experimental study on rabbit lamellar corneal transplantation with double-layer corneal stromal lens attactched by fibrin sealant
AIM: To study the biocompatibility of double-layer corneal stromal lens attactched by fibrin sealant(FS)in vivo, and to explore the feasibility of using this material for corneal transplantation.METHODS: Fifteen healthy and clean New Zealand white rabbits were selected for a self-control study. The...
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Press of International Journal of Ophthalmology (IJO PRESS)
2022-05-01
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| Series: | Guoji Yanke Zazhi |
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| Online Access: | http://ies.ijo.cn/cn_publish/2022/5/202205015.pdf |
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| author | Jia-Yue Ji Liu-Qing Wei Zacharia Ackbarkhan Jing Zeng |
| author_facet | Jia-Yue Ji Liu-Qing Wei Zacharia Ackbarkhan Jing Zeng |
| author_sort | Jia-Yue Ji |
| collection | DOAJ |
| description | AIM: To study the biocompatibility of double-layer corneal stromal lens attactched by fibrin sealant(FS)in vivo, and to explore the feasibility of using this material for corneal transplantation.METHODS: Fifteen healthy and clean New Zealand white rabbits were selected for a self-control study. The right eye of the rabbit was used as the experimental eye and the left eye was used as the control eye. The experimental eyes used FS adhesived double-layer corneal stromal lens as the material for lamellar keratoplasty, and the control eyes did not undergo manual intervention. At 7,14, and 28d after surgery, a hand-held slit lamp was used to observe the cornea of the rabbits and then score the biocompatibility. The corneas of both eyes were taken for histopathological examination by HE staining to observe the corneal recovery at the same time.RESULTS: Slit lamp observation results showed that by 28d after the operation, the corneal epithelium of the experimental eyes grew well, the degree of corneal transparency was basically restored, the degree of edema was reduced, the growth of neovascularization to the corneal edge was not aggravated, and no rejection reaction such as epithelial and endothelial rejection lines were seen; The control eyes had clear corneas and smooth corneal epithelium. The results of biocompatibility score showed that the degree of corneal implant edema gradually decreased, the transparency gradually recovered, the rejection reaction was less, and the biocompatibility of corneal implants was better in the experimental eyes after corneal transplantation. There were no differences in the degree of corneal transparency, edema and neovascularization growth between the experimental and control eyes at 28d after surgery(P>0.01). The results of histopathological examination showed that by 28d after corneal transplantation, there were 4-5 layers of corneal epithelial cells covering the surface of the implant in the experimental eyes, the corneal collagen was neatly and regularly arranged, no obvious inflammatory cell infiltration was seen in the implant, the boundary between the two lenses disappeared, the interlayer FS was completely absorbed by the organism, the implant was fused with the implant bed, and no obvious demarcation was seen.CONCLUSION:Using FS pasted double-layer corneal stroma lens as a graft for lamellar keratoplasty has better recovery, less rejection and better biocompatibility, and can be used for lamellar keratoplasty. |
| first_indexed | 2024-04-14T00:40:08Z |
| format | Article |
| id | doaj.art-ff442265b1cb4ef59739901e755d92ec |
| institution | Directory Open Access Journal |
| issn | 1672-5123 |
| language | English |
| last_indexed | 2024-04-14T00:40:08Z |
| publishDate | 2022-05-01 |
| publisher | Press of International Journal of Ophthalmology (IJO PRESS) |
| record_format | Article |
| series | Guoji Yanke Zazhi |
| spelling | doaj.art-ff442265b1cb4ef59739901e755d92ec2022-12-22T02:22:13ZengPress of International Journal of Ophthalmology (IJO PRESS)Guoji Yanke Zazhi1672-51232022-05-0122577577910.3980/j.issn.1672-5123.2022.5.15202205015Experimental study on rabbit lamellar corneal transplantation with double-layer corneal stromal lens attactched by fibrin sealantJia-Yue Ji0Liu-Qing Wei1Zacharia Ackbarkhan2Jing Zeng3Guangxi Medical University,Nanning 530021, Guangxi Zhuang Autonomous Region, ChinaGuangxi Medical University,Nanning 530021, Guangxi Zhuang Autonomous Region, ChinaGuangxi Medical University,Nanning 530021, Guangxi Zhuang Autonomous Region, ChinaDepartment of Ophthalmology, the First Affiliated Hospital of Guangxi Medical University, Nanning 530021, Guangxi Zhuang Autonomous Region, ChinaAIM: To study the biocompatibility of double-layer corneal stromal lens attactched by fibrin sealant(FS)in vivo, and to explore the feasibility of using this material for corneal transplantation.METHODS: Fifteen healthy and clean New Zealand white rabbits were selected for a self-control study. The right eye of the rabbit was used as the experimental eye and the left eye was used as the control eye. The experimental eyes used FS adhesived double-layer corneal stromal lens as the material for lamellar keratoplasty, and the control eyes did not undergo manual intervention. At 7,14, and 28d after surgery, a hand-held slit lamp was used to observe the cornea of the rabbits and then score the biocompatibility. The corneas of both eyes were taken for histopathological examination by HE staining to observe the corneal recovery at the same time.RESULTS: Slit lamp observation results showed that by 28d after the operation, the corneal epithelium of the experimental eyes grew well, the degree of corneal transparency was basically restored, the degree of edema was reduced, the growth of neovascularization to the corneal edge was not aggravated, and no rejection reaction such as epithelial and endothelial rejection lines were seen; The control eyes had clear corneas and smooth corneal epithelium. The results of biocompatibility score showed that the degree of corneal implant edema gradually decreased, the transparency gradually recovered, the rejection reaction was less, and the biocompatibility of corneal implants was better in the experimental eyes after corneal transplantation. There were no differences in the degree of corneal transparency, edema and neovascularization growth between the experimental and control eyes at 28d after surgery(P>0.01). The results of histopathological examination showed that by 28d after corneal transplantation, there were 4-5 layers of corneal epithelial cells covering the surface of the implant in the experimental eyes, the corneal collagen was neatly and regularly arranged, no obvious inflammatory cell infiltration was seen in the implant, the boundary between the two lenses disappeared, the interlayer FS was completely absorbed by the organism, the implant was fused with the implant bed, and no obvious demarcation was seen.CONCLUSION:Using FS pasted double-layer corneal stroma lens as a graft for lamellar keratoplasty has better recovery, less rejection and better biocompatibility, and can be used for lamellar keratoplasty.http://ies.ijo.cn/cn_publish/2022/5/202205015.pdfcorneal stromal lensfibrin sealantcorneal transplant materialrabbit lamellar corneal transplantationbiocompatibility |
| spellingShingle | Jia-Yue Ji Liu-Qing Wei Zacharia Ackbarkhan Jing Zeng Experimental study on rabbit lamellar corneal transplantation with double-layer corneal stromal lens attactched by fibrin sealant Guoji Yanke Zazhi corneal stromal lens fibrin sealant corneal transplant material rabbit lamellar corneal transplantation biocompatibility |
| title | Experimental study on rabbit lamellar corneal transplantation with double-layer corneal stromal lens attactched by fibrin sealant |
| title_full | Experimental study on rabbit lamellar corneal transplantation with double-layer corneal stromal lens attactched by fibrin sealant |
| title_fullStr | Experimental study on rabbit lamellar corneal transplantation with double-layer corneal stromal lens attactched by fibrin sealant |
| title_full_unstemmed | Experimental study on rabbit lamellar corneal transplantation with double-layer corneal stromal lens attactched by fibrin sealant |
| title_short | Experimental study on rabbit lamellar corneal transplantation with double-layer corneal stromal lens attactched by fibrin sealant |
| title_sort | experimental study on rabbit lamellar corneal transplantation with double layer corneal stromal lens attactched by fibrin sealant |
| topic | corneal stromal lens fibrin sealant corneal transplant material rabbit lamellar corneal transplantation biocompatibility |
| url | http://ies.ijo.cn/cn_publish/2022/5/202205015.pdf |
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