Analysis of Self-Incompatibility and Genetic Diversity in Diploid and Hexaploid Plum Genotypes
During the last decade, S-genotyping has been extensively investigated in fruit tree crops such as those belonging to the Prunus genus, including plums. In plums, S-allele typing has been largely studied in diploid species but works are scarcer in polyploid species due to the complexity of the polyp...
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| Format: | Article |
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Frontiers Media S.A.
2019-07-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | https://www.frontiersin.org/article/10.3389/fpls.2019.00896/full |
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| author | Donia Abdallah Ghada Baraket Veronica Perez Sana Ben Mustapha Amel Salhi-Hannachi Jose I. Hormaza |
| author_facet | Donia Abdallah Ghada Baraket Veronica Perez Sana Ben Mustapha Amel Salhi-Hannachi Jose I. Hormaza |
| author_sort | Donia Abdallah |
| collection | DOAJ |
| description | During the last decade, S-genotyping has been extensively investigated in fruit tree crops such as those belonging to the Prunus genus, including plums. In plums, S-allele typing has been largely studied in diploid species but works are scarcer in polyploid species due to the complexity of the polyploid genome. This study was conducted in order to analyze the S-genotypes of 30 diploid P. salicina, 17 of them reported here for the first time, and 29 hexaploid plums (24 of P. domestica and 5 of P. insititia). PCR analysis allowed identifying nine S-alleles in the P. salicina samples allocating the 30 accessions in 16 incompatibility groups, two of them identified here for the first time. In addition, pollen tube growth was studied in self-pollinated flowers of 17 Tunisian P. salicina under the microscope. In 16 samples, including one carrying the Se allele, which has been correlated with self-compatibility, the pollen tubes were arrested in the style. Only in one cultivar (“Bedri”), the pollen tubes reached the base of the style. Twelve S-alleles were identified in the 24 P. domestica and 5 P. insititia accessions, assigning accessions in 16 S-genotypes. S-genotyping results were combined with nine SSR loci to analyze genetic diversity. Results showed a close genetic relationship between P. domestica and P. salicina and between P. domestica and P. insititia corroborating that S-locus genotyping is suitable for molecular fingerprinting in diploid and polyploid Prunus species. |
| first_indexed | 2024-12-11T22:19:27Z |
| format | Article |
| id | doaj.art-ff45e3475a124d5cbb74404cfab5c153 |
| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-12-11T22:19:27Z |
| publishDate | 2019-07-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj.art-ff45e3475a124d5cbb74404cfab5c1532022-12-22T00:48:29ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2019-07-011010.3389/fpls.2019.00896451125Analysis of Self-Incompatibility and Genetic Diversity in Diploid and Hexaploid Plum GenotypesDonia Abdallah0Ghada Baraket1Veronica Perez2Sana Ben Mustapha3Amel Salhi-Hannachi4Jose I. Hormaza5Laboratoire de Génétique Moléculaire, Immunologie et Biotechnologie, Faculté des Sciences de Tunis, Université de Tunis El Manar, Tunis, TunisiaLaboratoire de Génétique Moléculaire, Immunologie et Biotechnologie, Faculté des Sciences de Tunis, Université de Tunis El Manar, Tunis, TunisiaUnidad Técnica del IPNA-CSIC, Laboratorio de Agrobiología Juan José Bravo Rodríguez (Cabildo Insular de La Palma), Santa Cruz de La Palma, SpainLaboratoire de Génétique Moléculaire, Immunologie et Biotechnologie, Faculté des Sciences de Tunis, Université de Tunis El Manar, Tunis, TunisiaLaboratoire de Génétique Moléculaire, Immunologie et Biotechnologie, Faculté des Sciences de Tunis, Université de Tunis El Manar, Tunis, TunisiaInstituto de Hortofruticultura Subtropical y Mediterránea La Mayora (IHSM La Mayora -UMA-CSIC), Algarrobo, SpainDuring the last decade, S-genotyping has been extensively investigated in fruit tree crops such as those belonging to the Prunus genus, including plums. In plums, S-allele typing has been largely studied in diploid species but works are scarcer in polyploid species due to the complexity of the polyploid genome. This study was conducted in order to analyze the S-genotypes of 30 diploid P. salicina, 17 of them reported here for the first time, and 29 hexaploid plums (24 of P. domestica and 5 of P. insititia). PCR analysis allowed identifying nine S-alleles in the P. salicina samples allocating the 30 accessions in 16 incompatibility groups, two of them identified here for the first time. In addition, pollen tube growth was studied in self-pollinated flowers of 17 Tunisian P. salicina under the microscope. In 16 samples, including one carrying the Se allele, which has been correlated with self-compatibility, the pollen tubes were arrested in the style. Only in one cultivar (“Bedri”), the pollen tubes reached the base of the style. Twelve S-alleles were identified in the 24 P. domestica and 5 P. insititia accessions, assigning accessions in 16 S-genotypes. S-genotyping results were combined with nine SSR loci to analyze genetic diversity. Results showed a close genetic relationship between P. domestica and P. salicina and between P. domestica and P. insititia corroborating that S-locus genotyping is suitable for molecular fingerprinting in diploid and polyploid Prunus species.https://www.frontiersin.org/article/10.3389/fpls.2019.00896/fullplumspolyploidyS-genotypingself-(in)compatibilitypollinationpollen tube |
| spellingShingle | Donia Abdallah Ghada Baraket Veronica Perez Sana Ben Mustapha Amel Salhi-Hannachi Jose I. Hormaza Analysis of Self-Incompatibility and Genetic Diversity in Diploid and Hexaploid Plum Genotypes Frontiers in Plant Science plums polyploidy S-genotyping self-(in)compatibility pollination pollen tube |
| title | Analysis of Self-Incompatibility and Genetic Diversity in Diploid and Hexaploid Plum Genotypes |
| title_full | Analysis of Self-Incompatibility and Genetic Diversity in Diploid and Hexaploid Plum Genotypes |
| title_fullStr | Analysis of Self-Incompatibility and Genetic Diversity in Diploid and Hexaploid Plum Genotypes |
| title_full_unstemmed | Analysis of Self-Incompatibility and Genetic Diversity in Diploid and Hexaploid Plum Genotypes |
| title_short | Analysis of Self-Incompatibility and Genetic Diversity in Diploid and Hexaploid Plum Genotypes |
| title_sort | analysis of self incompatibility and genetic diversity in diploid and hexaploid plum genotypes |
| topic | plums polyploidy S-genotyping self-(in)compatibility pollination pollen tube |
| url | https://www.frontiersin.org/article/10.3389/fpls.2019.00896/full |
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