Comparison of gross pathology inspection and 9.4 T magnetic resonance imaging in the evaluation of radiofrequency ablation lesions in the left ventricle of the swine heart
Aims: Gross pathology inspection (patho) is the gold standard for the morphological evaluation of focal myocardial pathology. Examination with 9.4 T magnetic resonance imaging (MRI) is a new method for very accurate display of myocardial pathology. The aim of this study was to demonstrate that lesio...
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Frontiers Media S.A.
2022-10-01
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| Series: | Frontiers in Physiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fphys.2022.834328/full |
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| author | Eva Odehnalová Lucia Valíková Guido Caluori Guido Caluori Guido Caluori Guido Caluori Tomáš Kulík Tomáš Kulík Veronika Římalová Tomasz Jadczyk Tomasz Jadczyk Eva Dražanová Iveta Pavlova Martin Pešl Martin Pešl Martin Pešl Václav Kubeš Zdeněk Stárek Zdeněk Stárek |
| author_facet | Eva Odehnalová Lucia Valíková Guido Caluori Guido Caluori Guido Caluori Guido Caluori Tomáš Kulík Tomáš Kulík Veronika Římalová Tomasz Jadczyk Tomasz Jadczyk Eva Dražanová Iveta Pavlova Martin Pešl Martin Pešl Martin Pešl Václav Kubeš Zdeněk Stárek Zdeněk Stárek |
| author_sort | Eva Odehnalová |
| collection | DOAJ |
| description | Aims: Gross pathology inspection (patho) is the gold standard for the morphological evaluation of focal myocardial pathology. Examination with 9.4 T magnetic resonance imaging (MRI) is a new method for very accurate display of myocardial pathology. The aim of this study was to demonstrate that lesions can be measured on high-resolution MRI images with the same accuracy as on pathological sections and compare these two methods for the evaluation of radiofrequency (RF) ablation lesion dimensions in swine heart tissue during animal experiment.Methods: Ten pigs underwent radiofrequency ablations in the left ventricle during animal experiment. After animal euthanasia, hearts were explanted, flushed with ice-cold cardioplegic solution to relax the whole myocardium, fixed in 10% formaldehyde and scanned with a 9.4 T magnetic resonance system. Anatomical images were processed using ImageJ software. Subsequently, the hearts were sliced, slices were photographed and measured in ImageJ software. Different dimensions and volumes were compared.Results: The results of both methods were statistically compared. Depth by MRI was 8.771 ± 2.595 mm and by patho 9.008 ± 2.823 mm; p = 0.198. Width was 10.802 ± 2.724 mm by MRI and 11.125 ± 2.801 mm by patho; p = 0.049. Estuary was 2.006 ± 0.867 mm by MRI and 2.001 ± 0.872 mm by patho; p = 0.953. The depth at the maximum diameter was 4.734 ± 1.532 mm on MRI and 4.783 ± 1.648 mm from the patho; p = 0.858. The volumes of the lesions calculated using a formula were 315.973 ± 257.673 mm3 for MRI and 355.726 ± 255.860 mm3 for patho; p = 0.104. Volume directly measured from MRI with the “point-by-point” method was 671.702 ± 362.299 mm3.Conclusion: Measurements obtained from gross pathology inspection and MRI are fully comparable. The advantage of MRI is that it is a non-destructive method enabling repeated measurements in all possible anatomical projections. |
| first_indexed | 2024-04-13T18:54:09Z |
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| language | English |
| last_indexed | 2024-04-13T18:54:09Z |
| publishDate | 2022-10-01 |
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| series | Frontiers in Physiology |
| spelling | doaj.art-ff4858e0728741f1806c081bc1bed39c2022-12-22T02:34:20ZengFrontiers Media S.A.Frontiers in Physiology1664-042X2022-10-011310.3389/fphys.2022.834328834328Comparison of gross pathology inspection and 9.4 T magnetic resonance imaging in the evaluation of radiofrequency ablation lesions in the left ventricle of the swine heartEva Odehnalová0Lucia Valíková1Guido Caluori2Guido Caluori3Guido Caluori4Guido Caluori5Tomáš Kulík6Tomáš Kulík7Veronika Římalová8Tomasz Jadczyk9Tomasz Jadczyk10Eva Dražanová11Iveta Pavlova12Martin Pešl13Martin Pešl14Martin Pešl15Václav Kubeš16Zdeněk Stárek17Zdeněk Stárek18Interventional Cardiac Electrophysiology Group, International Clinical Research Center, St. Anne’s University Hospital Brno, Brno, CzechInterventional Cardiac Electrophysiology Group, International Clinical Research Center, St. Anne’s University Hospital Brno, Brno, CzechInterventional Cardiac Electrophysiology Group, International Clinical Research Center, St. Anne’s University Hospital Brno, Brno, CzechNanotechnology, CEITEC Masaryk University, Brno, CzechIHU LIRYC, Electrophysiology and Heart Modeling Institute, Fondation Bordeaux Université, Pessac, FranceUniversity Bordeaux, INSERM, Cardiothoracic Research Center of Bordeaux, Pessac, FranceInterventional Cardiac Electrophysiology Group, International Clinical Research Center, St. Anne’s University Hospital Brno, Brno, Czech1st Department of Internal Medicine—Cardioangiology, St. Anne’s University Hospital Brno, Brno, CzechBiostatistics, International Clinical Research Center, St. Anne’s University Hospital Brno, Brno, CzechInterventional Cardiac Electrophysiology Group, International Clinical Research Center, St. Anne’s University Hospital Brno, Brno, CzechDivision of Cardiology and Structural Heart Diseases, Medical University of Silesia, Katowice, PolandInstitute of Scientific Instruments of the Czech Academy of Sciences, Brno, CzechInstitute of Scientific Instruments of the Czech Academy of Sciences, Brno, CzechInterventional Cardiac Electrophysiology Group, International Clinical Research Center, St. Anne’s University Hospital Brno, Brno, CzechNanotechnology, CEITEC Masaryk University, Brno, CzechDepartment of Biology, Faculty of Medicine Masaryk University Brno, Brno, Czech0Department of Pathology, University Hospital Brno, Brno, CzechInterventional Cardiac Electrophysiology Group, International Clinical Research Center, St. Anne’s University Hospital Brno, Brno, Czech1st Department of Internal Medicine—Cardioangiology, St. Anne’s University Hospital Brno, Brno, CzechAims: Gross pathology inspection (patho) is the gold standard for the morphological evaluation of focal myocardial pathology. Examination with 9.4 T magnetic resonance imaging (MRI) is a new method for very accurate display of myocardial pathology. The aim of this study was to demonstrate that lesions can be measured on high-resolution MRI images with the same accuracy as on pathological sections and compare these two methods for the evaluation of radiofrequency (RF) ablation lesion dimensions in swine heart tissue during animal experiment.Methods: Ten pigs underwent radiofrequency ablations in the left ventricle during animal experiment. After animal euthanasia, hearts were explanted, flushed with ice-cold cardioplegic solution to relax the whole myocardium, fixed in 10% formaldehyde and scanned with a 9.4 T magnetic resonance system. Anatomical images were processed using ImageJ software. Subsequently, the hearts were sliced, slices were photographed and measured in ImageJ software. Different dimensions and volumes were compared.Results: The results of both methods were statistically compared. Depth by MRI was 8.771 ± 2.595 mm and by patho 9.008 ± 2.823 mm; p = 0.198. Width was 10.802 ± 2.724 mm by MRI and 11.125 ± 2.801 mm by patho; p = 0.049. Estuary was 2.006 ± 0.867 mm by MRI and 2.001 ± 0.872 mm by patho; p = 0.953. The depth at the maximum diameter was 4.734 ± 1.532 mm on MRI and 4.783 ± 1.648 mm from the patho; p = 0.858. The volumes of the lesions calculated using a formula were 315.973 ± 257.673 mm3 for MRI and 355.726 ± 255.860 mm3 for patho; p = 0.104. Volume directly measured from MRI with the “point-by-point” method was 671.702 ± 362.299 mm3.Conclusion: Measurements obtained from gross pathology inspection and MRI are fully comparable. The advantage of MRI is that it is a non-destructive method enabling repeated measurements in all possible anatomical projections.https://www.frontiersin.org/articles/10.3389/fphys.2022.834328/fullevaluation of radiofrequency ablation lesionscomparison of methodsgross pathology inspectionhigh-resolution MRI evaluationanimal experimentscatheter ablation of arrhythmias |
| spellingShingle | Eva Odehnalová Lucia Valíková Guido Caluori Guido Caluori Guido Caluori Guido Caluori Tomáš Kulík Tomáš Kulík Veronika Římalová Tomasz Jadczyk Tomasz Jadczyk Eva Dražanová Iveta Pavlova Martin Pešl Martin Pešl Martin Pešl Václav Kubeš Zdeněk Stárek Zdeněk Stárek Comparison of gross pathology inspection and 9.4 T magnetic resonance imaging in the evaluation of radiofrequency ablation lesions in the left ventricle of the swine heart Frontiers in Physiology evaluation of radiofrequency ablation lesions comparison of methods gross pathology inspection high-resolution MRI evaluation animal experiments catheter ablation of arrhythmias |
| title | Comparison of gross pathology inspection and 9.4 T magnetic resonance imaging in the evaluation of radiofrequency ablation lesions in the left ventricle of the swine heart |
| title_full | Comparison of gross pathology inspection and 9.4 T magnetic resonance imaging in the evaluation of radiofrequency ablation lesions in the left ventricle of the swine heart |
| title_fullStr | Comparison of gross pathology inspection and 9.4 T magnetic resonance imaging in the evaluation of radiofrequency ablation lesions in the left ventricle of the swine heart |
| title_full_unstemmed | Comparison of gross pathology inspection and 9.4 T magnetic resonance imaging in the evaluation of radiofrequency ablation lesions in the left ventricle of the swine heart |
| title_short | Comparison of gross pathology inspection and 9.4 T magnetic resonance imaging in the evaluation of radiofrequency ablation lesions in the left ventricle of the swine heart |
| title_sort | comparison of gross pathology inspection and 9 4 t magnetic resonance imaging in the evaluation of radiofrequency ablation lesions in the left ventricle of the swine heart |
| topic | evaluation of radiofrequency ablation lesions comparison of methods gross pathology inspection high-resolution MRI evaluation animal experiments catheter ablation of arrhythmias |
| url | https://www.frontiersin.org/articles/10.3389/fphys.2022.834328/full |
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