<em>Paeonias</em> sp. variety Sesu <em>in vitro</em> multiplication in Temporary Immersion Systems
Paeonia are considered among the most important perennial ornamentals. The influence of three culture methods (semi-solid culture medium, static liquid culture medium and liquid culture medium by temporary immersion) as well as the type and concentration of cytokinins was investigated to improve the...
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Format: | Article |
Language: | English |
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Universidad Central Marta Abreu de Las Villas
2010-07-01
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Series: | Biotecnología Vegetal |
Online Access: | https://revista.ibp.co.cu/index.php/BV/article/view/284 |
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author | Yarianne Lezcano Maritza Escalona Marcos Daquinta |
author_facet | Yarianne Lezcano Maritza Escalona Marcos Daquinta |
author_sort | Yarianne Lezcano |
collection | DOAJ |
description | Paeonia are considered among the most important perennial ornamentals. The influence of three culture methods (semi-solid culture medium, static liquid culture medium and liquid culture medium by temporary immersion) as well as the type and concentration of cytokinins was investigated to improve the coefficients of in vitro multiplication of Paeonia sp. var. ‘SeSu’. Results demonstrated that temporary immersion increased significantly the multiplication coefficient by increasing the concentration of benzyladenine. A maximum concentration value of 6.66 mmol l-1 was reached. Both, type and concentration of cytokinins had effect on the multiplication of Paeonia var. SESu in its temporary immersion. The highest rates of multiplication were achieved with concentrations of 4.44 and 6.66 mmol l-1 meta-topolin and 6.66 mmol l-1 BA, without significant differences between them. Both cytokinins enhanced the shoot length. Hyperhydric shoots were not found.
Key words: cytokinins, ornamentals, proliferation |
first_indexed | 2024-12-10T23:30:20Z |
format | Article |
id | doaj.art-ff4c478a27054be1a233f745b4f114c2 |
institution | Directory Open Access Journal |
issn | 1609-1841 2074-8647 |
language | English |
last_indexed | 2024-12-10T23:30:20Z |
publishDate | 2010-07-01 |
publisher | Universidad Central Marta Abreu de Las Villas |
record_format | Article |
series | Biotecnología Vegetal |
spelling | doaj.art-ff4c478a27054be1a233f745b4f114c22022-12-22T01:29:25ZengUniversidad Central Marta Abreu de Las VillasBiotecnología Vegetal1609-18412074-86472010-07-01103258<em>Paeonias</em> sp. variety Sesu <em>in vitro</em> multiplication in Temporary Immersion SystemsYarianne LezcanoMaritza EscalonaMarcos DaquintaPaeonia are considered among the most important perennial ornamentals. The influence of three culture methods (semi-solid culture medium, static liquid culture medium and liquid culture medium by temporary immersion) as well as the type and concentration of cytokinins was investigated to improve the coefficients of in vitro multiplication of Paeonia sp. var. ‘SeSu’. Results demonstrated that temporary immersion increased significantly the multiplication coefficient by increasing the concentration of benzyladenine. A maximum concentration value of 6.66 mmol l-1 was reached. Both, type and concentration of cytokinins had effect on the multiplication of Paeonia var. SESu in its temporary immersion. The highest rates of multiplication were achieved with concentrations of 4.44 and 6.66 mmol l-1 meta-topolin and 6.66 mmol l-1 BA, without significant differences between them. Both cytokinins enhanced the shoot length. Hyperhydric shoots were not found. Key words: cytokinins, ornamentals, proliferationhttps://revista.ibp.co.cu/index.php/BV/article/view/284 |
spellingShingle | Yarianne Lezcano Maritza Escalona Marcos Daquinta <em>Paeonias</em> sp. variety Sesu <em>in vitro</em> multiplication in Temporary Immersion Systems Biotecnología Vegetal |
title | <em>Paeonias</em> sp. variety Sesu <em>in vitro</em> multiplication in Temporary Immersion Systems |
title_full | <em>Paeonias</em> sp. variety Sesu <em>in vitro</em> multiplication in Temporary Immersion Systems |
title_fullStr | <em>Paeonias</em> sp. variety Sesu <em>in vitro</em> multiplication in Temporary Immersion Systems |
title_full_unstemmed | <em>Paeonias</em> sp. variety Sesu <em>in vitro</em> multiplication in Temporary Immersion Systems |
title_short | <em>Paeonias</em> sp. variety Sesu <em>in vitro</em> multiplication in Temporary Immersion Systems |
title_sort | em paeonias em sp variety sesu em in vitro em multiplication in temporary immersion systems |
url | https://revista.ibp.co.cu/index.php/BV/article/view/284 |
work_keys_str_mv | AT yariannelezcano empaeoniasemspvarietysesueminvitroemmultiplicationintemporaryimmersionsystems AT maritzaescalona empaeoniasemspvarietysesueminvitroemmultiplicationintemporaryimmersionsystems AT marcosdaquinta empaeoniasemspvarietysesueminvitroemmultiplicationintemporaryimmersionsystems |