<em>Paeonias</em> sp. variety Sesu <em>in vitro</em> multiplication in Temporary Immersion Systems

Paeonia are considered among the most important perennial ornamentals. The influence of three culture methods (semi-solid culture medium, static liquid culture medium and liquid culture medium by temporary immersion) as well as the type and concentration of cytokinins was investigated to improve the...

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Main Authors: Yarianne Lezcano, Maritza Escalona, Marcos Daquinta
Format: Article
Language:English
Published: Universidad Central Marta Abreu de Las Villas 2010-07-01
Series:Biotecnología Vegetal
Online Access:https://revista.ibp.co.cu/index.php/BV/article/view/284
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author Yarianne Lezcano
Maritza Escalona
Marcos Daquinta
author_facet Yarianne Lezcano
Maritza Escalona
Marcos Daquinta
author_sort Yarianne Lezcano
collection DOAJ
description Paeonia are considered among the most important perennial ornamentals. The influence of three culture methods (semi-solid culture medium, static liquid culture medium and liquid culture medium by temporary immersion) as well as the type and concentration of cytokinins was investigated to improve the coefficients of in vitro multiplication of Paeonia sp. var. ‘SeSu’. Results demonstrated that temporary immersion increased significantly the multiplication coefficient by increasing the concentration of benzyladenine. A maximum concentration value of 6.66 mmol l-1 was reached. Both, type and concentration of cytokinins had effect on the multiplication of Paeonia var. SESu in its temporary immersion. The highest rates of multiplication were achieved with concentrations of 4.44 and 6.66 mmol l-1 meta-topolin and 6.66 mmol l-1 BA, without significant differences between them. Both cytokinins enhanced the shoot length. Hyperhydric shoots were not found. Key words: cytokinins, ornamentals, proliferation
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spelling doaj.art-ff4c478a27054be1a233f745b4f114c22022-12-22T01:29:25ZengUniversidad Central Marta Abreu de Las VillasBiotecnología Vegetal1609-18412074-86472010-07-01103258<em>Paeonias</em> sp. variety Sesu <em>in vitro</em> multiplication in Temporary Immersion SystemsYarianne LezcanoMaritza EscalonaMarcos DaquintaPaeonia are considered among the most important perennial ornamentals. The influence of three culture methods (semi-solid culture medium, static liquid culture medium and liquid culture medium by temporary immersion) as well as the type and concentration of cytokinins was investigated to improve the coefficients of in vitro multiplication of Paeonia sp. var. ‘SeSu’. Results demonstrated that temporary immersion increased significantly the multiplication coefficient by increasing the concentration of benzyladenine. A maximum concentration value of 6.66 mmol l-1 was reached. Both, type and concentration of cytokinins had effect on the multiplication of Paeonia var. SESu in its temporary immersion. The highest rates of multiplication were achieved with concentrations of 4.44 and 6.66 mmol l-1 meta-topolin and 6.66 mmol l-1 BA, without significant differences between them. Both cytokinins enhanced the shoot length. Hyperhydric shoots were not found. Key words: cytokinins, ornamentals, proliferationhttps://revista.ibp.co.cu/index.php/BV/article/view/284
spellingShingle Yarianne Lezcano
Maritza Escalona
Marcos Daquinta
<em>Paeonias</em> sp. variety Sesu <em>in vitro</em> multiplication in Temporary Immersion Systems
Biotecnología Vegetal
title <em>Paeonias</em> sp. variety Sesu <em>in vitro</em> multiplication in Temporary Immersion Systems
title_full <em>Paeonias</em> sp. variety Sesu <em>in vitro</em> multiplication in Temporary Immersion Systems
title_fullStr <em>Paeonias</em> sp. variety Sesu <em>in vitro</em> multiplication in Temporary Immersion Systems
title_full_unstemmed <em>Paeonias</em> sp. variety Sesu <em>in vitro</em> multiplication in Temporary Immersion Systems
title_short <em>Paeonias</em> sp. variety Sesu <em>in vitro</em> multiplication in Temporary Immersion Systems
title_sort em paeonias em sp variety sesu em in vitro em multiplication in temporary immersion systems
url https://revista.ibp.co.cu/index.php/BV/article/view/284
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AT maritzaescalona empaeoniasemspvarietysesueminvitroemmultiplicationintemporaryimmersionsystems
AT marcosdaquinta empaeoniasemspvarietysesueminvitroemmultiplicationintemporaryimmersionsystems