Decontamination of sputum for longer time in sodium hydroxide for isolation of Mycobacterium tuberculosis
Decontamination by modified Petroff's method is being practiced in many laboratories carrying out Mycobacterium tuberculosis culture and drug susceptibility testing. The method exposes mycobacteria to 4% sodium hydroxide for 30 min. However, laboratories in developing countries with limited res...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Wolters Kluwer Medknow Publications
2014-01-01
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| Series: | International Journal of Mycobacteriology |
| Subjects: | |
| Online Access: | http://www.ijmyco.org/article.asp?issn=2212-5531;year=2014;volume=3;issue=4;spage=290;epage=292;aulast=Satapathy |
| _version_ | 1818484208002138112 |
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| author | P Satapathy D Das B N Murmu S K Kar |
| author_facet | P Satapathy D Das B N Murmu S K Kar |
| author_sort | P Satapathy |
| collection | DOAJ |
| description | Decontamination by modified Petroff's method is being practiced in many laboratories carrying out Mycobacterium tuberculosis culture and drug susceptibility testing. The method exposes mycobacteria to 4% sodium hydroxide for 30 min. However, laboratories in developing countries with limited resources might be using a type of centrifuge that does not open during power failures and exposes the mycobacteria to alkali for longer periods. Out of 28 smear-positive specimens processed, 85.7%, 85.7% and 60.7% of specimens showed a positive culture after exposure to alkali for 0.5, 1.0 and 72h. Laboratories compelled to expose the mycobacteria for a longer duration of time can still attempt isolation for culture as only a small amount of bacteria are needed for culture positivity. |
| first_indexed | 2024-12-10T15:52:04Z |
| format | Article |
| id | doaj.art-ff4ca47c7e824299a6756fe690dae5e8 |
| institution | Directory Open Access Journal |
| issn | 2212-5531 2212-554X |
| language | English |
| last_indexed | 2024-12-10T15:52:04Z |
| publishDate | 2014-01-01 |
| publisher | Wolters Kluwer Medknow Publications |
| record_format | Article |
| series | International Journal of Mycobacteriology |
| spelling | doaj.art-ff4ca47c7e824299a6756fe690dae5e82022-12-22T01:42:47ZengWolters Kluwer Medknow PublicationsInternational Journal of Mycobacteriology2212-55312212-554X2014-01-013429029210.1016/j.ijmyco.2014.09.006Decontamination of sputum for longer time in sodium hydroxide for isolation of Mycobacterium tuberculosisP SatapathyD DasB N MurmuS K KarDecontamination by modified Petroff's method is being practiced in many laboratories carrying out Mycobacterium tuberculosis culture and drug susceptibility testing. The method exposes mycobacteria to 4% sodium hydroxide for 30 min. However, laboratories in developing countries with limited resources might be using a type of centrifuge that does not open during power failures and exposes the mycobacteria to alkali for longer periods. Out of 28 smear-positive specimens processed, 85.7%, 85.7% and 60.7% of specimens showed a positive culture after exposure to alkali for 0.5, 1.0 and 72h. Laboratories compelled to expose the mycobacteria for a longer duration of time can still attempt isolation for culture as only a small amount of bacteria are needed for culture positivity.http://www.ijmyco.org/article.asp?issn=2212-5531;year=2014;volume=3;issue=4;spage=290;epage=292;aulast=SatapathyTuberculosisModified Petroff's methodSputum |
| spellingShingle | P Satapathy D Das B N Murmu S K Kar Decontamination of sputum for longer time in sodium hydroxide for isolation of Mycobacterium tuberculosis International Journal of Mycobacteriology Tuberculosis Modified Petroff's method Sputum |
| title | Decontamination of sputum for longer time in sodium hydroxide for isolation of Mycobacterium tuberculosis |
| title_full | Decontamination of sputum for longer time in sodium hydroxide for isolation of Mycobacterium tuberculosis |
| title_fullStr | Decontamination of sputum for longer time in sodium hydroxide for isolation of Mycobacterium tuberculosis |
| title_full_unstemmed | Decontamination of sputum for longer time in sodium hydroxide for isolation of Mycobacterium tuberculosis |
| title_short | Decontamination of sputum for longer time in sodium hydroxide for isolation of Mycobacterium tuberculosis |
| title_sort | decontamination of sputum for longer time in sodium hydroxide for isolation of mycobacterium tuberculosis |
| topic | Tuberculosis Modified Petroff's method Sputum |
| url | http://www.ijmyco.org/article.asp?issn=2212-5531;year=2014;volume=3;issue=4;spage=290;epage=292;aulast=Satapathy |
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