Differential expression patterns of phospholipase D isoforms 1 and 2 in the mammalian brain and retina
Phosphatidic acid is a key signaling molecule heavily implicated in exocytosis due to its protein-binding partners and propensity to induce negative membrane curvature. One phosphatidic acid-producing enzyme, phospholipase D (PLD), has also been implicated in neurotransmission. Unfortunately, due to...
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| Format: | Article |
| Language: | English |
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Elsevier
2022-08-01
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| Series: | Journal of Lipid Research |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S0022227522000803 |
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| author | Casey N. Barber Hana L. Goldschmidt Brendan Lilley Alexei M. Bygrave Richard C. Johnson Richard L. Huganir Donald J. Zack Daniel M. Raben |
| author_facet | Casey N. Barber Hana L. Goldschmidt Brendan Lilley Alexei M. Bygrave Richard C. Johnson Richard L. Huganir Donald J. Zack Daniel M. Raben |
| author_sort | Casey N. Barber |
| collection | DOAJ |
| description | Phosphatidic acid is a key signaling molecule heavily implicated in exocytosis due to its protein-binding partners and propensity to induce negative membrane curvature. One phosphatidic acid-producing enzyme, phospholipase D (PLD), has also been implicated in neurotransmission. Unfortunately, due to the unreliability of reagents, there has been confusion in the literature regarding the expression of PLD isoforms in the mammalian brain which has hampered our understanding of their functional roles in neurons. To address this, we generated epitope-tagged PLD1 and PLD2 knockin mice using CRISPR/Cas9. Using these mice, we show that PLD1 and PLD2 are both localized at synapses by adulthood, with PLD2 expression being considerably higher in glial cells and PLD1 expression predominating in neurons. Interestingly, we observed that only PLD1 is expressed in the mouse retina, where it is found in the synaptic plexiform layers. These data provide critical information regarding the localization and potential role of PLDs in the central nervous system. |
| first_indexed | 2024-04-14T02:33:56Z |
| format | Article |
| id | doaj.art-ff4db0915c4049ca95f94efafaacc188 |
| institution | Directory Open Access Journal |
| issn | 0022-2275 |
| language | English |
| last_indexed | 2024-04-14T02:33:56Z |
| publishDate | 2022-08-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Lipid Research |
| spelling | doaj.art-ff4db0915c4049ca95f94efafaacc1882022-12-22T02:17:36ZengElsevierJournal of Lipid Research0022-22752022-08-01638100247Differential expression patterns of phospholipase D isoforms 1 and 2 in the mammalian brain and retinaCasey N. Barber0Hana L. Goldschmidt1Brendan Lilley2Alexei M. Bygrave3Richard C. Johnson4Richard L. Huganir5Donald J. Zack6Daniel M. Raben7Department of Biological Chemistry, The Johns Hopkins University School of Medicine, Baltimore, MD, USA; Solomon H. Snyder Department of Neuroscience, The Johns Hopkins University School of Medicine, Baltimore, MD, USASolomon H. Snyder Department of Neuroscience, The Johns Hopkins University School of Medicine, Baltimore, MD, USADepartment of Ophthalmology, Wilmer Eye Institute, The Johns Hopkins University School of Medicine, Baltimore, MD, USASolomon H. Snyder Department of Neuroscience, The Johns Hopkins University School of Medicine, Baltimore, MD, USASolomon H. Snyder Department of Neuroscience, The Johns Hopkins University School of Medicine, Baltimore, MD, USASolomon H. Snyder Department of Neuroscience, The Johns Hopkins University School of Medicine, Baltimore, MD, USADepartment of Ophthalmology, Wilmer Eye Institute, The Johns Hopkins University School of Medicine, Baltimore, MD, USADepartment of Biological Chemistry, The Johns Hopkins University School of Medicine, Baltimore, MD, USA; For correspondence: Daniel M. RabenPhosphatidic acid is a key signaling molecule heavily implicated in exocytosis due to its protein-binding partners and propensity to induce negative membrane curvature. One phosphatidic acid-producing enzyme, phospholipase D (PLD), has also been implicated in neurotransmission. Unfortunately, due to the unreliability of reagents, there has been confusion in the literature regarding the expression of PLD isoforms in the mammalian brain which has hampered our understanding of their functional roles in neurons. To address this, we generated epitope-tagged PLD1 and PLD2 knockin mice using CRISPR/Cas9. Using these mice, we show that PLD1 and PLD2 are both localized at synapses by adulthood, with PLD2 expression being considerably higher in glial cells and PLD1 expression predominating in neurons. Interestingly, we observed that only PLD1 is expressed in the mouse retina, where it is found in the synaptic plexiform layers. These data provide critical information regarding the localization and potential role of PLDs in the central nervous system.http://www.sciencedirect.com/science/article/pii/S0022227522000803phospholipids/phosphatidic acidbrain lipidseye/retinaphospholipases/Dexocytosisneurotransmission |
| spellingShingle | Casey N. Barber Hana L. Goldschmidt Brendan Lilley Alexei M. Bygrave Richard C. Johnson Richard L. Huganir Donald J. Zack Daniel M. Raben Differential expression patterns of phospholipase D isoforms 1 and 2 in the mammalian brain and retina Journal of Lipid Research phospholipids/phosphatidic acid brain lipids eye/retina phospholipases/D exocytosis neurotransmission |
| title | Differential expression patterns of phospholipase D isoforms 1 and 2 in the mammalian brain and retina |
| title_full | Differential expression patterns of phospholipase D isoforms 1 and 2 in the mammalian brain and retina |
| title_fullStr | Differential expression patterns of phospholipase D isoforms 1 and 2 in the mammalian brain and retina |
| title_full_unstemmed | Differential expression patterns of phospholipase D isoforms 1 and 2 in the mammalian brain and retina |
| title_short | Differential expression patterns of phospholipase D isoforms 1 and 2 in the mammalian brain and retina |
| title_sort | differential expression patterns of phospholipase d isoforms 1 and 2 in the mammalian brain and retina |
| topic | phospholipids/phosphatidic acid brain lipids eye/retina phospholipases/D exocytosis neurotransmission |
| url | http://www.sciencedirect.com/science/article/pii/S0022227522000803 |
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