Re-Programing Glucose Catabolism in the Microalga <i>Chlorella sorokiniana</i> under Light Condition
The microalga <i>Chlorella sorokiniana</i> has attracted much attention for lipid production and wastewater treatment. It can perform photosynthesis and organic carbon utilization concurrently. To understand its phototrophic metabolism, a biomass compositional analysis, a <sup>13&l...
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| Format: | Article |
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MDPI AG
2022-07-01
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| Series: | Biomolecules |
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| Online Access: | https://www.mdpi.com/2218-273X/12/7/939 |
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| author | Tingting Li Na Pang Lian He Yuan Xu Xinyu Fu Yinjie Tang Yair Shachar-Hill Shulin Chen |
| author_facet | Tingting Li Na Pang Lian He Yuan Xu Xinyu Fu Yinjie Tang Yair Shachar-Hill Shulin Chen |
| author_sort | Tingting Li |
| collection | DOAJ |
| description | The microalga <i>Chlorella sorokiniana</i> has attracted much attention for lipid production and wastewater treatment. It can perform photosynthesis and organic carbon utilization concurrently. To understand its phototrophic metabolism, a biomass compositional analysis, a <sup>13</sup>C metabolic flux analysis, and metabolite pool size analyses were performed. Under dark condition, the oxidative pentose phosphate pathway (OPP) was the major route for glucose catabolism (88% carbon flux) and a cyclic OPP–glycolytic route for glucose catabolism was formed. Under light condition, fluxes in the glucose catabolism, tricarboxylic acid (TCA) cycle, and anaplerotic reaction (CO<sub>2</sub> fixation via phosphoenolpyruvate carboxylase) were all suppressed. Meanwhile, the RuBisCO reaction became active and the ratio of its carbon fixation to glucose carbon utilization was determined as 7:100. Moreover, light condition significantly reduced the pool sizes of sugar phosphate metabolites (such as E4P, F6P, and S7P) and promoted biomass synthesis (which reached 0.155 h<sup>−1</sup>). In addition, light condition increased glucose consumption rates, leading to higher ATP and NADPH production and a higher protein content (43% vs. 30%) in the biomass during the exponential growth phase. |
| first_indexed | 2024-03-09T10:22:48Z |
| format | Article |
| id | doaj.art-ff513621e446419ebdbc7b069088d9fb |
| institution | Directory Open Access Journal |
| issn | 2218-273X |
| language | English |
| last_indexed | 2024-03-09T10:22:48Z |
| publishDate | 2022-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biomolecules |
| spelling | doaj.art-ff513621e446419ebdbc7b069088d9fb2023-12-01T21:55:31ZengMDPI AGBiomolecules2218-273X2022-07-0112793910.3390/biom12070939Re-Programing Glucose Catabolism in the Microalga <i>Chlorella sorokiniana</i> under Light ConditionTingting Li0Na Pang1Lian He2Yuan Xu3Xinyu Fu4Yinjie Tang5Yair Shachar-Hill6Shulin Chen7Department of Immunology, School of Basic Medical Sciences, Lanzhou University, Lanzhou 730000, ChinaDepartment of Biological Systems Engineering, Washington State University, Pullman, WA 99164, USADepartment of Energy, Environmental and Chemical Engineering, Washington University, St. Louis, MO 63130, USADepartment of Plant Biology, Michigan State University, East Lansing, MI 48823, USADepartment of Energy-Plant Research Laboratory, Michigan State University, East Lansing, MI 48823, USADepartment of Energy, Environmental and Chemical Engineering, Washington University, St. Louis, MO 63130, USADepartment of Plant Biology, Michigan State University, East Lansing, MI 48823, USADepartment of Biological Systems Engineering, Washington State University, Pullman, WA 99164, USAThe microalga <i>Chlorella sorokiniana</i> has attracted much attention for lipid production and wastewater treatment. It can perform photosynthesis and organic carbon utilization concurrently. To understand its phototrophic metabolism, a biomass compositional analysis, a <sup>13</sup>C metabolic flux analysis, and metabolite pool size analyses were performed. Under dark condition, the oxidative pentose phosphate pathway (OPP) was the major route for glucose catabolism (88% carbon flux) and a cyclic OPP–glycolytic route for glucose catabolism was formed. Under light condition, fluxes in the glucose catabolism, tricarboxylic acid (TCA) cycle, and anaplerotic reaction (CO<sub>2</sub> fixation via phosphoenolpyruvate carboxylase) were all suppressed. Meanwhile, the RuBisCO reaction became active and the ratio of its carbon fixation to glucose carbon utilization was determined as 7:100. Moreover, light condition significantly reduced the pool sizes of sugar phosphate metabolites (such as E4P, F6P, and S7P) and promoted biomass synthesis (which reached 0.155 h<sup>−1</sup>). In addition, light condition increased glucose consumption rates, leading to higher ATP and NADPH production and a higher protein content (43% vs. 30%) in the biomass during the exponential growth phase.https://www.mdpi.com/2218-273X/12/7/939microalgae<i>Chlorella sorokiniana</i><sup>13</sup>C-MFAmixotrophicheterotrophicpool size |
| spellingShingle | Tingting Li Na Pang Lian He Yuan Xu Xinyu Fu Yinjie Tang Yair Shachar-Hill Shulin Chen Re-Programing Glucose Catabolism in the Microalga <i>Chlorella sorokiniana</i> under Light Condition Biomolecules microalgae <i>Chlorella sorokiniana</i> <sup>13</sup>C-MFA mixotrophic heterotrophic pool size |
| title | Re-Programing Glucose Catabolism in the Microalga <i>Chlorella sorokiniana</i> under Light Condition |
| title_full | Re-Programing Glucose Catabolism in the Microalga <i>Chlorella sorokiniana</i> under Light Condition |
| title_fullStr | Re-Programing Glucose Catabolism in the Microalga <i>Chlorella sorokiniana</i> under Light Condition |
| title_full_unstemmed | Re-Programing Glucose Catabolism in the Microalga <i>Chlorella sorokiniana</i> under Light Condition |
| title_short | Re-Programing Glucose Catabolism in the Microalga <i>Chlorella sorokiniana</i> under Light Condition |
| title_sort | re programing glucose catabolism in the microalga i chlorella sorokiniana i under light condition |
| topic | microalgae <i>Chlorella sorokiniana</i> <sup>13</sup>C-MFA mixotrophic heterotrophic pool size |
| url | https://www.mdpi.com/2218-273X/12/7/939 |
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