Zinc-Doped Bioactive Glass/Polycaprolactone Hybrid Scaffolds Manufactured by Direct and Indirect 3D Printing Methods for Bone Regeneration
A novel organic–inorganic hybrid, based on SiO<sub>2</sub>-CaO-ZnO bioactive glass (BG) and polycaprolactone (PCL), associating the highly bioactive and versatile bioactive glass with clinically established PCL was examined. The BG–PCL hybrid is obtained by acid-catalyzed silica sol–gel...
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| Format: | Article |
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MDPI AG
2023-06-01
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| Series: | Cells |
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| Online Access: | https://www.mdpi.com/2073-4409/12/13/1759 |
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| author | Nafise Elahpour Isabella Niesner Cédric Bossard Nora Abdellaoui Valérie Montouillout Franck Fayon Christine Taviot-Guého Tina Frankenbach Alexander Crispin Pardis Khosravani Boris Michael Holzapfel Edouard Jallot Susanne Mayer-Wagner Jonathan Lao |
| author_facet | Nafise Elahpour Isabella Niesner Cédric Bossard Nora Abdellaoui Valérie Montouillout Franck Fayon Christine Taviot-Guého Tina Frankenbach Alexander Crispin Pardis Khosravani Boris Michael Holzapfel Edouard Jallot Susanne Mayer-Wagner Jonathan Lao |
| author_sort | Nafise Elahpour |
| collection | DOAJ |
| description | A novel organic–inorganic hybrid, based on SiO<sub>2</sub>-CaO-ZnO bioactive glass (BG) and polycaprolactone (PCL), associating the highly bioactive and versatile bioactive glass with clinically established PCL was examined. The BG–PCL hybrid is obtained by acid-catalyzed silica sol–gel process inside PCL solution either by direct or indirect printing. Apatite-formation tests in simulated body fluid (SBF) confirm the ion release along with the hybrid’s bone-like apatite forming. Kinetics differ significantly between directly and indirectly printed scaffolds, the former requiring longer periods to degrade, while the latter demonstrates faster calcium phosphate (CaP) formation. Remarkably, Zn diffusion and accumulation are observed at the surface within the newly formed active CaP layer. Zn release is found to be dependent on printing method and immersion medium. Investigation of BG at the atomic scale reveals the ambivalent role of Zn, capable of acting both as a network modifier and as a network former linking the BG silicate network. In addition, hMSCs viability assay proves no cytotoxicity of the Zn hybrid. LIVE/DEAD staining demonstrated excellent cell viability and proliferation for over seven weeks. Overall, this hybrid material either non-doped or doped with a metal trace element is a promising candidate to be translated to clinical applications for bone regeneration. |
| first_indexed | 2024-03-11T01:45:43Z |
| format | Article |
| id | doaj.art-ff51dde6e74b42cdb6b259388a6b9905 |
| institution | Directory Open Access Journal |
| issn | 2073-4409 |
| language | English |
| last_indexed | 2024-03-11T01:45:43Z |
| publishDate | 2023-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Cells |
| spelling | doaj.art-ff51dde6e74b42cdb6b259388a6b99052023-11-18T16:19:39ZengMDPI AGCells2073-44092023-06-011213175910.3390/cells12131759Zinc-Doped Bioactive Glass/Polycaprolactone Hybrid Scaffolds Manufactured by Direct and Indirect 3D Printing Methods for Bone RegenerationNafise Elahpour0Isabella Niesner1Cédric Bossard2Nora Abdellaoui3Valérie Montouillout4Franck Fayon5Christine Taviot-Guého6Tina Frankenbach7Alexander Crispin8Pardis Khosravani9Boris Michael Holzapfel10Edouard Jallot11Susanne Mayer-Wagner12Jonathan Lao13Laboratoire de Physique de Clermont (LPC), Université Clermont Auvergne, CNRS/IN2P3, F-63000 Clermont-Ferrand, FranceDepartment of Orthopaedics and Trauma Surgery, Musculoskeletal University Center Munich (MUM), LMU University Hospital, LMU Munich, 80336 Munich, GermanyLaboratoire de Physique de Clermont (LPC), Université Clermont Auvergne, CNRS/IN2P3, F-63000 Clermont-Ferrand, FranceLaboratoire de Physique de Clermont (LPC), Université Clermont Auvergne, CNRS/IN2P3, F-63000 Clermont-Ferrand, FranceConditions Extrêmes et Matériaux: Haute Température et Irradiation (CEMHTI), CNRS-UPR3079, Université Orléans, F-45071 Orléans, FranceConditions Extrêmes et Matériaux: Haute Température et Irradiation (CEMHTI), CNRS-UPR3079, Université Orléans, F-45071 Orléans, FranceInstitut de Chimie de Clermont-Ferrand, Université Clermont Auvergne, CNRS/UMR 6296, F-63000 Clermont-Ferrand, FranceDepartment of Orthopaedics and Trauma Surgery, Musculoskeletal University Center Munich (MUM), LMU University Hospital, LMU Munich, 80336 Munich, GermanyInstitute for Medical Information Processing, Biometry, and Epidemiology (IBE), Ludwig-Maximilians-Universität München, 81377 Munich, GermanyFlow Cytometry Core Facility, Biomedical Center, Ludwig-Maximilians-Universität München, 82152 Planegg, GermanyDepartment of Orthopaedics and Trauma Surgery, Musculoskeletal University Center Munich (MUM), LMU University Hospital, LMU Munich, 80336 Munich, GermanyLaboratoire de Physique de Clermont (LPC), Université Clermont Auvergne, CNRS/IN2P3, F-63000 Clermont-Ferrand, FranceDepartment of Orthopaedics and Trauma Surgery, Musculoskeletal University Center Munich (MUM), LMU University Hospital, LMU Munich, 80336 Munich, GermanyLaboratoire de Physique de Clermont (LPC), Université Clermont Auvergne, CNRS/IN2P3, F-63000 Clermont-Ferrand, FranceA novel organic–inorganic hybrid, based on SiO<sub>2</sub>-CaO-ZnO bioactive glass (BG) and polycaprolactone (PCL), associating the highly bioactive and versatile bioactive glass with clinically established PCL was examined. The BG–PCL hybrid is obtained by acid-catalyzed silica sol–gel process inside PCL solution either by direct or indirect printing. Apatite-formation tests in simulated body fluid (SBF) confirm the ion release along with the hybrid’s bone-like apatite forming. Kinetics differ significantly between directly and indirectly printed scaffolds, the former requiring longer periods to degrade, while the latter demonstrates faster calcium phosphate (CaP) formation. Remarkably, Zn diffusion and accumulation are observed at the surface within the newly formed active CaP layer. Zn release is found to be dependent on printing method and immersion medium. Investigation of BG at the atomic scale reveals the ambivalent role of Zn, capable of acting both as a network modifier and as a network former linking the BG silicate network. In addition, hMSCs viability assay proves no cytotoxicity of the Zn hybrid. LIVE/DEAD staining demonstrated excellent cell viability and proliferation for over seven weeks. Overall, this hybrid material either non-doped or doped with a metal trace element is a promising candidate to be translated to clinical applications for bone regeneration.https://www.mdpi.com/2073-4409/12/13/1759sol–gelbioactive glassorganic-inorganic hybridadditive manufacturinghuman mesenchymal stem cellsbone tissue engineering |
| spellingShingle | Nafise Elahpour Isabella Niesner Cédric Bossard Nora Abdellaoui Valérie Montouillout Franck Fayon Christine Taviot-Guého Tina Frankenbach Alexander Crispin Pardis Khosravani Boris Michael Holzapfel Edouard Jallot Susanne Mayer-Wagner Jonathan Lao Zinc-Doped Bioactive Glass/Polycaprolactone Hybrid Scaffolds Manufactured by Direct and Indirect 3D Printing Methods for Bone Regeneration Cells sol–gel bioactive glass organic-inorganic hybrid additive manufacturing human mesenchymal stem cells bone tissue engineering |
| title | Zinc-Doped Bioactive Glass/Polycaprolactone Hybrid Scaffolds Manufactured by Direct and Indirect 3D Printing Methods for Bone Regeneration |
| title_full | Zinc-Doped Bioactive Glass/Polycaprolactone Hybrid Scaffolds Manufactured by Direct and Indirect 3D Printing Methods for Bone Regeneration |
| title_fullStr | Zinc-Doped Bioactive Glass/Polycaprolactone Hybrid Scaffolds Manufactured by Direct and Indirect 3D Printing Methods for Bone Regeneration |
| title_full_unstemmed | Zinc-Doped Bioactive Glass/Polycaprolactone Hybrid Scaffolds Manufactured by Direct and Indirect 3D Printing Methods for Bone Regeneration |
| title_short | Zinc-Doped Bioactive Glass/Polycaprolactone Hybrid Scaffolds Manufactured by Direct and Indirect 3D Printing Methods for Bone Regeneration |
| title_sort | zinc doped bioactive glass polycaprolactone hybrid scaffolds manufactured by direct and indirect 3d printing methods for bone regeneration |
| topic | sol–gel bioactive glass organic-inorganic hybrid additive manufacturing human mesenchymal stem cells bone tissue engineering |
| url | https://www.mdpi.com/2073-4409/12/13/1759 |
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