Short or Long Interval between Priming and Boosting: Does It Impact on the Vaccine Immunogenicity?
Characterizing the impact of the vaccination schedule on the induction of B and T cell immune responses is critical for improving vaccine immunogenicity. Here we compare the effect of a short (4 weeks) or a long (18 weeks) interval between priming and boosting in mice, using a model vaccine formulat...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-03-01
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| Series: | Vaccines |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2076-393X/9/3/289 |
| _version_ | 1827697244194734080 |
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| author | Elena Pettini Gabiria Pastore Fabio Fiorino Donata Medaglini Annalisa Ciabattini |
| author_facet | Elena Pettini Gabiria Pastore Fabio Fiorino Donata Medaglini Annalisa Ciabattini |
| author_sort | Elena Pettini |
| collection | DOAJ |
| description | Characterizing the impact of the vaccination schedule on the induction of B and T cell immune responses is critical for improving vaccine immunogenicity. Here we compare the effect of a short (4 weeks) or a long (18 weeks) interval between priming and boosting in mice, using a model vaccine formulation based on the chimeric tuberculosis vaccine antigen H56 combined with alum. While no significant difference was observed in serum antigen-specific IgG response and the induction of antigen-specific T follicular helper cells into draining lymph nodes after the two immunization schedules, a longer interval between priming and boosting elicited a higher number of germinal center-B cells and H56-specific antibody-secreting cells and modulated the effector function of reactivated CD4+ T cells. These data show that the scheduling of the booster immunization could affect the immune response elicited by vaccination modulating and improving the immunogenicity of the vaccine. |
| first_indexed | 2024-03-10T13:04:25Z |
| format | Article |
| id | doaj.art-ff540fd75e3f4486ab7d5f72d1aa9e57 |
| institution | Directory Open Access Journal |
| issn | 2076-393X |
| language | English |
| last_indexed | 2024-03-10T13:04:25Z |
| publishDate | 2021-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Vaccines |
| spelling | doaj.art-ff540fd75e3f4486ab7d5f72d1aa9e572023-11-21T11:16:42ZengMDPI AGVaccines2076-393X2021-03-019328910.3390/vaccines9030289Short or Long Interval between Priming and Boosting: Does It Impact on the Vaccine Immunogenicity?Elena Pettini0Gabiria Pastore1Fabio Fiorino2Donata Medaglini3Annalisa Ciabattini4Laboratory of Molecular Microbiology and Biotechnology (LA.M.M.B.), Department of Medical Biotechnologies, University of Siena, 53100 Siena, ItalyLaboratory of Molecular Microbiology and Biotechnology (LA.M.M.B.), Department of Medical Biotechnologies, University of Siena, 53100 Siena, ItalyLaboratory of Molecular Microbiology and Biotechnology (LA.M.M.B.), Department of Medical Biotechnologies, University of Siena, 53100 Siena, ItalyLaboratory of Molecular Microbiology and Biotechnology (LA.M.M.B.), Department of Medical Biotechnologies, University of Siena, 53100 Siena, ItalyLaboratory of Molecular Microbiology and Biotechnology (LA.M.M.B.), Department of Medical Biotechnologies, University of Siena, 53100 Siena, ItalyCharacterizing the impact of the vaccination schedule on the induction of B and T cell immune responses is critical for improving vaccine immunogenicity. Here we compare the effect of a short (4 weeks) or a long (18 weeks) interval between priming and boosting in mice, using a model vaccine formulation based on the chimeric tuberculosis vaccine antigen H56 combined with alum. While no significant difference was observed in serum antigen-specific IgG response and the induction of antigen-specific T follicular helper cells into draining lymph nodes after the two immunization schedules, a longer interval between priming and boosting elicited a higher number of germinal center-B cells and H56-specific antibody-secreting cells and modulated the effector function of reactivated CD4+ T cells. These data show that the scheduling of the booster immunization could affect the immune response elicited by vaccination modulating and improving the immunogenicity of the vaccine.https://www.mdpi.com/2076-393X/9/3/289immunizationalumB cell responseT cell responseantibodiesprime–boost schedules |
| spellingShingle | Elena Pettini Gabiria Pastore Fabio Fiorino Donata Medaglini Annalisa Ciabattini Short or Long Interval between Priming and Boosting: Does It Impact on the Vaccine Immunogenicity? Vaccines immunization alum B cell response T cell response antibodies prime–boost schedules |
| title | Short or Long Interval between Priming and Boosting: Does It Impact on the Vaccine Immunogenicity? |
| title_full | Short or Long Interval between Priming and Boosting: Does It Impact on the Vaccine Immunogenicity? |
| title_fullStr | Short or Long Interval between Priming and Boosting: Does It Impact on the Vaccine Immunogenicity? |
| title_full_unstemmed | Short or Long Interval between Priming and Boosting: Does It Impact on the Vaccine Immunogenicity? |
| title_short | Short or Long Interval between Priming and Boosting: Does It Impact on the Vaccine Immunogenicity? |
| title_sort | short or long interval between priming and boosting does it impact on the vaccine immunogenicity |
| topic | immunization alum B cell response T cell response antibodies prime–boost schedules |
| url | https://www.mdpi.com/2076-393X/9/3/289 |
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