Novel Genomic Locus with Atypical G+C Content that Is Required for Extracellular Polysaccharide Production and Virulence in Xanthomonas oryzae pv. oryzae
Three exopolysaccharide (EPS)- and virulence-deficient mutants of Xanthomonas oryzae pv. oryzae, the causal agent of bacterial leaf blight of rice, were isolated by Tn5 mutagenesis. These insertions are not located within the gum gene cluster. A 40-kb cosmid clone that restored EPS production and vi...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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The American Phytopathological Society
2001-11-01
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| Series: | Molecular Plant-Microbe Interactions |
| Online Access: | https://apsjournals.apsnet.org/doi/10.1094/MPMI.2001.14.11.1335 |
| _version_ | 1818915154193022976 |
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| author | Sridhar Dharmapuri J. Yashitola M. R. Vishnupriya Ramesh V. Sonti |
| author_facet | Sridhar Dharmapuri J. Yashitola M. R. Vishnupriya Ramesh V. Sonti |
| author_sort | Sridhar Dharmapuri |
| collection | DOAJ |
| description | Three exopolysaccharide (EPS)- and virulence-deficient mutants of Xanthomonas oryzae pv. oryzae, the causal agent of bacterial leaf blight of rice, were isolated by Tn5 mutagenesis. These insertions are not located within the gum gene cluster. A 40-kb cosmid clone that restored EPS production and virulence to all three mutants was isolated, and the three transposon insertions were localized to contiguous 4.3- and 3.5-kb EcoRI fragments that are included in this clone. Sequence data indicate that two of the transposon insertions are in genes that encode a putative sugar nucleotide epimerase and a putative glycosyl transferase, respectively; the third insertion is located between the glycosyl transferase gene and a novel open reading frame (ORF). A 5.5-kb genomic region in which these three ORFs are located has a G+C content of 51.7%, quite different from the G+C content of approximately 65.0% that is typical of X. oryzae pv. oryzae. Homologues of this locus have not yet been reported in any other xanthomonad. |
| first_indexed | 2024-12-19T23:57:46Z |
| format | Article |
| id | doaj.art-ff54cf01ce8f4738adcf688c808972d7 |
| institution | Directory Open Access Journal |
| issn | 0894-0282 1943-7706 |
| language | English |
| last_indexed | 2024-12-19T23:57:46Z |
| publishDate | 2001-11-01 |
| publisher | The American Phytopathological Society |
| record_format | Article |
| series | Molecular Plant-Microbe Interactions |
| spelling | doaj.art-ff54cf01ce8f4738adcf688c808972d72022-12-21T20:00:57ZengThe American Phytopathological SocietyMolecular Plant-Microbe Interactions0894-02821943-77062001-11-0114111335133910.1094/MPMI.2001.14.11.1335Novel Genomic Locus with Atypical G+C Content that Is Required for Extracellular Polysaccharide Production and Virulence in Xanthomonas oryzae pv. oryzaeSridhar DharmapuriJ. YashitolaM. R. VishnupriyaRamesh V. SontiThree exopolysaccharide (EPS)- and virulence-deficient mutants of Xanthomonas oryzae pv. oryzae, the causal agent of bacterial leaf blight of rice, were isolated by Tn5 mutagenesis. These insertions are not located within the gum gene cluster. A 40-kb cosmid clone that restored EPS production and virulence to all three mutants was isolated, and the three transposon insertions were localized to contiguous 4.3- and 3.5-kb EcoRI fragments that are included in this clone. Sequence data indicate that two of the transposon insertions are in genes that encode a putative sugar nucleotide epimerase and a putative glycosyl transferase, respectively; the third insertion is located between the glycosyl transferase gene and a novel open reading frame (ORF). A 5.5-kb genomic region in which these three ORFs are located has a G+C content of 51.7%, quite different from the G+C content of approximately 65.0% that is typical of X. oryzae pv. oryzae. Homologues of this locus have not yet been reported in any other xanthomonad.https://apsjournals.apsnet.org/doi/10.1094/MPMI.2001.14.11.1335 |
| spellingShingle | Sridhar Dharmapuri J. Yashitola M. R. Vishnupriya Ramesh V. Sonti Novel Genomic Locus with Atypical G+C Content that Is Required for Extracellular Polysaccharide Production and Virulence in Xanthomonas oryzae pv. oryzae Molecular Plant-Microbe Interactions |
| title | Novel Genomic Locus with Atypical G+C Content that Is Required for Extracellular Polysaccharide Production and Virulence in Xanthomonas oryzae pv. oryzae |
| title_full | Novel Genomic Locus with Atypical G+C Content that Is Required for Extracellular Polysaccharide Production and Virulence in Xanthomonas oryzae pv. oryzae |
| title_fullStr | Novel Genomic Locus with Atypical G+C Content that Is Required for Extracellular Polysaccharide Production and Virulence in Xanthomonas oryzae pv. oryzae |
| title_full_unstemmed | Novel Genomic Locus with Atypical G+C Content that Is Required for Extracellular Polysaccharide Production and Virulence in Xanthomonas oryzae pv. oryzae |
| title_short | Novel Genomic Locus with Atypical G+C Content that Is Required for Extracellular Polysaccharide Production and Virulence in Xanthomonas oryzae pv. oryzae |
| title_sort | novel genomic locus with atypical g c content that is required for extracellular polysaccharide production and virulence in xanthomonas oryzae pv oryzae |
| url | https://apsjournals.apsnet.org/doi/10.1094/MPMI.2001.14.11.1335 |
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