Sertoli Cells Loaded with Doxorubicin in Lipid Micelles Reduced Tumor Burden and Dox-Induced Toxicity
The toxic side effects of doxorubicin (Dox) limit its long-term use as a lung cancer chemotherapeutic. Additionally, drug delivery to the deep lung is challenging. To address these challenges, isolated rat Sertoli cells (SCs) were preloaded with Dox conjugated to lipid micelle nanoparticles (SC-DLMN...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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SAGE Publishing
2017-10-01
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| Series: | Cell Transplantation |
| Online Access: | https://doi.org/10.1177/0963689717721223 |
| _version_ | 1818777441111375872 |
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| author | Mahasweta Das Mark Howell Elspeth A. Foran Rohit Iyre Shyam S. Mohapatra Subhra Mohapatra |
| author_facet | Mahasweta Das Mark Howell Elspeth A. Foran Rohit Iyre Shyam S. Mohapatra Subhra Mohapatra |
| author_sort | Mahasweta Das |
| collection | DOAJ |
| description | The toxic side effects of doxorubicin (Dox) limit its long-term use as a lung cancer chemotherapeutic. Additionally, drug delivery to the deep lung is challenging. To address these challenges, isolated rat Sertoli cells (SCs) were preloaded with Dox conjugated to lipid micelle nanoparticles (SC-DLMNs) and delivered to mouse lungs. These immunocompetent cells, when injected intravenously, travel to the lung, deliver the payload, and get cleared by the system quickly without causing any adverse reaction. We observed that SC-DLMNs effectively treated Lewis lung carcinoma 1-induced lung tumors in mice and the drug efficacy was comparable to SC-Dox treatment. Mice treated with SC-DLMNs also showed significantly less toxicity compared to those treated with SC-Dox. The encapsulation of Dox in lipid micelle nanoparticles reduced the toxicity of Dox and the SC-based delivery method ensured drug delivery to the deep lung without evoking any immune response. Taken together, these results provide a novel SC-based nanoparticle drug delivery method for improved therapeutic outcome of cardiotoxic antilung cancer drugs. |
| first_indexed | 2024-12-18T11:28:52Z |
| format | Article |
| id | doaj.art-ff553ff3d156495ab075d9f91e868960 |
| institution | Directory Open Access Journal |
| issn | 0963-6897 1555-3892 |
| language | English |
| last_indexed | 2024-12-18T11:28:52Z |
| publishDate | 2017-10-01 |
| publisher | SAGE Publishing |
| record_format | Article |
| series | Cell Transplantation |
| spelling | doaj.art-ff553ff3d156495ab075d9f91e8689602022-12-21T21:09:38ZengSAGE PublishingCell Transplantation0963-68971555-38922017-10-012610.1177/0963689717721223Sertoli Cells Loaded with Doxorubicin in Lipid Micelles Reduced Tumor Burden and Dox-Induced ToxicityMahasweta Das0Mark Howell1Elspeth A. Foran2Rohit Iyre3Shyam S. Mohapatra4Subhra Mohapatra5 Department of Internal Medicine, University of South Florida College of Medicine, Tampa, FL, USA Department of Molecular Medicine, University of South Florida College of Medicine, Tampa, FL, USA Department of Molecular Medicine, University of South Florida College of Medicine, Tampa, FL, USA Department of Internal Medicine, University of South Florida College of Medicine, Tampa, FL, USA James A. Haley Veterans Hospital, Tampa, FL, USA James A. Haley Veterans Hospital, Tampa, FL, USAThe toxic side effects of doxorubicin (Dox) limit its long-term use as a lung cancer chemotherapeutic. Additionally, drug delivery to the deep lung is challenging. To address these challenges, isolated rat Sertoli cells (SCs) were preloaded with Dox conjugated to lipid micelle nanoparticles (SC-DLMNs) and delivered to mouse lungs. These immunocompetent cells, when injected intravenously, travel to the lung, deliver the payload, and get cleared by the system quickly without causing any adverse reaction. We observed that SC-DLMNs effectively treated Lewis lung carcinoma 1-induced lung tumors in mice and the drug efficacy was comparable to SC-Dox treatment. Mice treated with SC-DLMNs also showed significantly less toxicity compared to those treated with SC-Dox. The encapsulation of Dox in lipid micelle nanoparticles reduced the toxicity of Dox and the SC-based delivery method ensured drug delivery to the deep lung without evoking any immune response. Taken together, these results provide a novel SC-based nanoparticle drug delivery method for improved therapeutic outcome of cardiotoxic antilung cancer drugs.https://doi.org/10.1177/0963689717721223 |
| spellingShingle | Mahasweta Das Mark Howell Elspeth A. Foran Rohit Iyre Shyam S. Mohapatra Subhra Mohapatra Sertoli Cells Loaded with Doxorubicin in Lipid Micelles Reduced Tumor Burden and Dox-Induced Toxicity Cell Transplantation |
| title | Sertoli Cells Loaded with Doxorubicin in Lipid Micelles Reduced Tumor Burden and Dox-Induced Toxicity |
| title_full | Sertoli Cells Loaded with Doxorubicin in Lipid Micelles Reduced Tumor Burden and Dox-Induced Toxicity |
| title_fullStr | Sertoli Cells Loaded with Doxorubicin in Lipid Micelles Reduced Tumor Burden and Dox-Induced Toxicity |
| title_full_unstemmed | Sertoli Cells Loaded with Doxorubicin in Lipid Micelles Reduced Tumor Burden and Dox-Induced Toxicity |
| title_short | Sertoli Cells Loaded with Doxorubicin in Lipid Micelles Reduced Tumor Burden and Dox-Induced Toxicity |
| title_sort | sertoli cells loaded with doxorubicin in lipid micelles reduced tumor burden and dox induced toxicity |
| url | https://doi.org/10.1177/0963689717721223 |
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