Characterization and Quantification of Arsenic Species in Foodstuffs of Plant Origin by HPLC/ICP-MS
Arsenic is a well-known carcinogenic, mutagenic and toxic element and occurs in the environment both as inorganic arsenic (iAs) and organoarsenical compounds (oAsCs). Since the toxicity of arsenic compounds depends on their chemical form, the identification and determination of arsenic species are e...
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MDPI AG
2023-02-01
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| author | Teresa D’Amore Oto Miedico Ciro Pompa Chiara Preite Marco Iammarino Valeria Nardelli |
| author_facet | Teresa D’Amore Oto Miedico Ciro Pompa Chiara Preite Marco Iammarino Valeria Nardelli |
| author_sort | Teresa D’Amore |
| collection | DOAJ |
| description | Arsenic is a well-known carcinogenic, mutagenic and toxic element and occurs in the environment both as inorganic arsenic (iAs) and organoarsenical compounds (oAsCs). Since the toxicity of arsenic compounds depends on their chemical form, the identification and determination of arsenic species are essential. Recently, the European Food Safety Authority, following the European Commission request, published a report on chronic dietary exposure to iAs and recommended the development and validation of analytical methods with adequate sensitivity and refined extraction procedures for this determination. Moreover, the authority called upon new arsenic speciation data for complex food matrices such as seaweeds, grains and grain-based products. Looking at this context, an optimized, sensitive and fast analytical method using high performance liquid chromatography followed by inductively coupled plasma—mass spectrometry (HPLC/ICP-MS) was developed for the determination of iAs (sum of arsenite—As<sup>III</sup> and arsenate—As<sup>V</sup>) and the most relevant oAsCs, arsenobetaine, dimethylarsinic acid and monomethylarsonic acid. The method was validated with satisfactory results in terms of linearity, sensitivity, selectivity, precision, recovery, uncertainty, ruggedness and matrix effect, and then successfully applied for the analysis of several matrices, i.e., processed and unprocessed cereal and cereal products, fruits, vegetables, legumes, seaweeds, nuts and seeds. The results obtained indicate that not only seaweed and rice matrices but also many cereals, legumes and plant-based foods for infants and young children contain significant concentrations of iAs and oAsCs. These findings contribute to the data collection necessary to assess the role of these matrices in the total arsenic exposure and if specific maximum limits have to be established. |
| first_indexed | 2024-03-11T08:33:08Z |
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| last_indexed | 2024-03-11T08:33:08Z |
| publishDate | 2023-02-01 |
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| series | Life |
| spelling | doaj.art-ff57209751434805b143f945081165ae2023-11-16T21:42:17ZengMDPI AGLife2075-17292023-02-0113251110.3390/life13020511Characterization and Quantification of Arsenic Species in Foodstuffs of Plant Origin by HPLC/ICP-MSTeresa D’Amore0Oto Miedico1Ciro Pompa2Chiara Preite3Marco Iammarino4Valeria Nardelli5Department of Chemistry, Istituto Zooprofilattico Sperimentale della Puglia e della Basilicata, Via Manfredonia 20, 71121 Foggia, ItalyDepartment of Chemistry, Istituto Zooprofilattico Sperimentale della Puglia e della Basilicata, Via Manfredonia 20, 71121 Foggia, ItalyDepartment of Chemistry, Istituto Zooprofilattico Sperimentale della Puglia e della Basilicata, Via Manfredonia 20, 71121 Foggia, ItalyDepartment of Chemistry, Istituto Zooprofilattico Sperimentale della Puglia e della Basilicata, Via Manfredonia 20, 71121 Foggia, ItalyDepartment of Chemistry, Istituto Zooprofilattico Sperimentale della Puglia e della Basilicata, Via Manfredonia 20, 71121 Foggia, ItalyDepartment of Chemistry, Istituto Zooprofilattico Sperimentale della Puglia e della Basilicata, Via Manfredonia 20, 71121 Foggia, ItalyArsenic is a well-known carcinogenic, mutagenic and toxic element and occurs in the environment both as inorganic arsenic (iAs) and organoarsenical compounds (oAsCs). Since the toxicity of arsenic compounds depends on their chemical form, the identification and determination of arsenic species are essential. Recently, the European Food Safety Authority, following the European Commission request, published a report on chronic dietary exposure to iAs and recommended the development and validation of analytical methods with adequate sensitivity and refined extraction procedures for this determination. Moreover, the authority called upon new arsenic speciation data for complex food matrices such as seaweeds, grains and grain-based products. Looking at this context, an optimized, sensitive and fast analytical method using high performance liquid chromatography followed by inductively coupled plasma—mass spectrometry (HPLC/ICP-MS) was developed for the determination of iAs (sum of arsenite—As<sup>III</sup> and arsenate—As<sup>V</sup>) and the most relevant oAsCs, arsenobetaine, dimethylarsinic acid and monomethylarsonic acid. The method was validated with satisfactory results in terms of linearity, sensitivity, selectivity, precision, recovery, uncertainty, ruggedness and matrix effect, and then successfully applied for the analysis of several matrices, i.e., processed and unprocessed cereal and cereal products, fruits, vegetables, legumes, seaweeds, nuts and seeds. The results obtained indicate that not only seaweed and rice matrices but also many cereals, legumes and plant-based foods for infants and young children contain significant concentrations of iAs and oAsCs. These findings contribute to the data collection necessary to assess the role of these matrices in the total arsenic exposure and if specific maximum limits have to be established.https://www.mdpi.com/2075-1729/13/2/511arsenicHPLC/ICP-MSspeciationplant-based foodsinfant foodshazard identification |
| spellingShingle | Teresa D’Amore Oto Miedico Ciro Pompa Chiara Preite Marco Iammarino Valeria Nardelli Characterization and Quantification of Arsenic Species in Foodstuffs of Plant Origin by HPLC/ICP-MS Life arsenic HPLC/ICP-MS speciation plant-based foods infant foods hazard identification |
| title | Characterization and Quantification of Arsenic Species in Foodstuffs of Plant Origin by HPLC/ICP-MS |
| title_full | Characterization and Quantification of Arsenic Species in Foodstuffs of Plant Origin by HPLC/ICP-MS |
| title_fullStr | Characterization and Quantification of Arsenic Species in Foodstuffs of Plant Origin by HPLC/ICP-MS |
| title_full_unstemmed | Characterization and Quantification of Arsenic Species in Foodstuffs of Plant Origin by HPLC/ICP-MS |
| title_short | Characterization and Quantification of Arsenic Species in Foodstuffs of Plant Origin by HPLC/ICP-MS |
| title_sort | characterization and quantification of arsenic species in foodstuffs of plant origin by hplc icp ms |
| topic | arsenic HPLC/ICP-MS speciation plant-based foods infant foods hazard identification |
| url | https://www.mdpi.com/2075-1729/13/2/511 |
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