Coastal Wild Grapevine Accession (<i>Vitis vinifera</i> L. ssp. <i>sylvestris</i>) Shows Distinct Late and Early Transcriptome Changes under Salt Stress in Comparison to Commercial Rootstock Richter 110
Increase in soil salinity, driven by climate change, is a widespread constrain for viticulture across several regions, including the Mediterranean basin. The implementation of salt-tolerant varieties is sought after to reduce the negative impact of salinity in grape production. An accession of wild...
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MDPI AG
2022-10-01
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| Series: | Plants |
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| Online Access: | https://www.mdpi.com/2223-7747/11/20/2688 |
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| author | David Carrasco Andres Zhou-Tsang Alberto Rodriguez-Izquierdo Rafael Ocete María Angeles Revilla Rosa Arroyo-García |
| author_facet | David Carrasco Andres Zhou-Tsang Alberto Rodriguez-Izquierdo Rafael Ocete María Angeles Revilla Rosa Arroyo-García |
| author_sort | David Carrasco |
| collection | DOAJ |
| description | Increase in soil salinity, driven by climate change, is a widespread constrain for viticulture across several regions, including the Mediterranean basin. The implementation of salt-tolerant varieties is sought after to reduce the negative impact of salinity in grape production. An accession of wild grapevine (<i>Vitis vinifera</i> L. ssp. <i>sylvestris</i>), named AS1B, found on the coastline of Asturias (Spain), could be of interest toward the achievement of salt-tolerant varieties, as it demonstrated the ability to survive and grow under high levels of salinity. In the present study, AS1B is compared against widely cultivated commercial rootstock Richter 110, regarding their survival capabilities, and transcriptomic profiles analysis allowed us to identify the genes by employing RNA-seq and gene ontology analyses under increasing salinity and validate (via RT-qPCR) seven salinity-stress-induced genes. The results suggest contrasting transcriptomic responses between AS1B and Richter 110. AS1B is more responsive to a milder increase in salinity and builds up specific mechanisms of tolerance over a sustained salt stress, while Richter 110 maintains a constitutive expression until high and prolonged saline inputs, when it mainly shows responses to osmotic stress. The genetic basis of AS1B’s strategy to confront salinity could be valuable in cultivar breeding programs, to expand the current range of salt-tolerant rootstocks, aiming to improve the adaptation of viticulture against climate change. |
| first_indexed | 2024-03-09T19:34:12Z |
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| institution | Directory Open Access Journal |
| issn | 2223-7747 |
| language | English |
| last_indexed | 2024-03-09T19:34:12Z |
| publishDate | 2022-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Plants |
| spelling | doaj.art-ff574e673980476581337be6f57886902023-11-24T02:03:33ZengMDPI AGPlants2223-77472022-10-011120268810.3390/plants11202688Coastal Wild Grapevine Accession (<i>Vitis vinifera</i> L. ssp. <i>sylvestris</i>) Shows Distinct Late and Early Transcriptome Changes under Salt Stress in Comparison to Commercial Rootstock Richter 110David Carrasco0Andres Zhou-Tsang1Alberto Rodriguez-Izquierdo2Rafael Ocete3María Angeles Revilla4Rosa Arroyo-García5CSIC-INIA(CBGP) Centro de Biotecnología y Genómica de Plantas, UPM-INIA, Parque Científico y Tecnológico de la UPM Campus de Montegancedo, CtraM-40, Km 38, Pozuelo de Alarcón, 28223 Madrid, SpainCSIC-INIA(CBGP) Centro de Biotecnología y Genómica de Plantas, UPM-INIA, Parque Científico y Tecnológico de la UPM Campus de Montegancedo, CtraM-40, Km 38, Pozuelo de Alarcón, 28223 Madrid, SpainCSIC-INIA(CBGP) Centro de Biotecnología y Genómica de Plantas, UPM-INIA, Parque Científico y Tecnológico de la UPM Campus de Montegancedo, CtraM-40, Km 38, Pozuelo de Alarcón, 28223 Madrid, SpainLaboratorio Entomología Aplicada, Universidad de Sevilla, Avenida Reina Mercedes 6, 41012 Sevilla, SpainDepartamento Biología de Organismos y Sistemas, Facultad de Biología, Universidad de Oviedo, 33071 Oviedo, SpainCSIC-INIA(CBGP) Centro de Biotecnología y Genómica de Plantas, UPM-INIA, Parque Científico y Tecnológico de la UPM Campus de Montegancedo, CtraM-40, Km 38, Pozuelo de Alarcón, 28223 Madrid, SpainIncrease in soil salinity, driven by climate change, is a widespread constrain for viticulture across several regions, including the Mediterranean basin. The implementation of salt-tolerant varieties is sought after to reduce the negative impact of salinity in grape production. An accession of wild grapevine (<i>Vitis vinifera</i> L. ssp. <i>sylvestris</i>), named AS1B, found on the coastline of Asturias (Spain), could be of interest toward the achievement of salt-tolerant varieties, as it demonstrated the ability to survive and grow under high levels of salinity. In the present study, AS1B is compared against widely cultivated commercial rootstock Richter 110, regarding their survival capabilities, and transcriptomic profiles analysis allowed us to identify the genes by employing RNA-seq and gene ontology analyses under increasing salinity and validate (via RT-qPCR) seven salinity-stress-induced genes. The results suggest contrasting transcriptomic responses between AS1B and Richter 110. AS1B is more responsive to a milder increase in salinity and builds up specific mechanisms of tolerance over a sustained salt stress, while Richter 110 maintains a constitutive expression until high and prolonged saline inputs, when it mainly shows responses to osmotic stress. The genetic basis of AS1B’s strategy to confront salinity could be valuable in cultivar breeding programs, to expand the current range of salt-tolerant rootstocks, aiming to improve the adaptation of viticulture against climate change.https://www.mdpi.com/2223-7747/11/20/2688wild grapevine accessionrootstocksalinitysalt tolerancetranscriptomic analysisgene ontology |
| spellingShingle | David Carrasco Andres Zhou-Tsang Alberto Rodriguez-Izquierdo Rafael Ocete María Angeles Revilla Rosa Arroyo-García Coastal Wild Grapevine Accession (<i>Vitis vinifera</i> L. ssp. <i>sylvestris</i>) Shows Distinct Late and Early Transcriptome Changes under Salt Stress in Comparison to Commercial Rootstock Richter 110 Plants wild grapevine accession rootstock salinity salt tolerance transcriptomic analysis gene ontology |
| title | Coastal Wild Grapevine Accession (<i>Vitis vinifera</i> L. ssp. <i>sylvestris</i>) Shows Distinct Late and Early Transcriptome Changes under Salt Stress in Comparison to Commercial Rootstock Richter 110 |
| title_full | Coastal Wild Grapevine Accession (<i>Vitis vinifera</i> L. ssp. <i>sylvestris</i>) Shows Distinct Late and Early Transcriptome Changes under Salt Stress in Comparison to Commercial Rootstock Richter 110 |
| title_fullStr | Coastal Wild Grapevine Accession (<i>Vitis vinifera</i> L. ssp. <i>sylvestris</i>) Shows Distinct Late and Early Transcriptome Changes under Salt Stress in Comparison to Commercial Rootstock Richter 110 |
| title_full_unstemmed | Coastal Wild Grapevine Accession (<i>Vitis vinifera</i> L. ssp. <i>sylvestris</i>) Shows Distinct Late and Early Transcriptome Changes under Salt Stress in Comparison to Commercial Rootstock Richter 110 |
| title_short | Coastal Wild Grapevine Accession (<i>Vitis vinifera</i> L. ssp. <i>sylvestris</i>) Shows Distinct Late and Early Transcriptome Changes under Salt Stress in Comparison to Commercial Rootstock Richter 110 |
| title_sort | coastal wild grapevine accession i vitis vinifera i l ssp i sylvestris i shows distinct late and early transcriptome changes under salt stress in comparison to commercial rootstock richter 110 |
| topic | wild grapevine accession rootstock salinity salt tolerance transcriptomic analysis gene ontology |
| url | https://www.mdpi.com/2223-7747/11/20/2688 |
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