Mitogenomes Provide Insights into the Species Boundaries and Phylogenetic Relationships among Three <i>Dolycoris</i> Sloe Bugs (Hemiptera: Pentatomidae) from China
(1) Background: The three sloe bugs, <i>Dolycoris baccarum</i>, <i>Dolycoris indicus,</i> and <i>Dolycoris penicillatus</i>, are found in the Chinese mainland and are morphologically similar. The species boundaries and phylogenetic relationships of the three speci...
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MDPI AG
2024-02-01
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| Series: | Insects |
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| Online Access: | https://www.mdpi.com/2075-4450/15/2/134 |
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| author | Chenguang Zheng Xiuxiu Zhu Ying Wang Xue Dong Ruijuan Yang Zechen Tang Wenjun Bu |
| author_facet | Chenguang Zheng Xiuxiu Zhu Ying Wang Xue Dong Ruijuan Yang Zechen Tang Wenjun Bu |
| author_sort | Chenguang Zheng |
| collection | DOAJ |
| description | (1) Background: The three sloe bugs, <i>Dolycoris baccarum</i>, <i>Dolycoris indicus,</i> and <i>Dolycoris penicillatus</i>, are found in the Chinese mainland and are morphologically similar. The species boundaries and phylogenetic relationships of the three species remain uncertain; (2) Methods: In this study, we generated multiple mitochondrial genomes (mitogenomes) for each of the three species and conducted comparative mitogenomic analysis, species delimitation, and phylogenetic analysis based on these data; (3) Results: Mitogenomes of the three <i>Dolycoris</i> species are conserved in nucleotide composition, gene arrangement, and codon usage. All protein-coding genes (PCGs) were found to be under purifying selection, and the ND4 evolved at the fastest rate. Most species delimitation analyses based on the COI gene and the concatenated 13 PCGs retrieved three operational taxonomic units (OTUs), which corresponded well with the three <i>Dolycoris</i> species identified based on morphological characters. A clear-cut barcode gap was discovered between the interspecific and intraspecific genetic distances of the three <i>Dolycoris</i> species. Phylogenetic analyses strongly supported the monophyly of <i>Dolycoris</i>, with interspecific relationship inferred as (<i>D. indicus</i> + (<i>D. baccarum</i> + <i>D. penicillatus</i>)); (4) Conclusions: Our study provides the first insight into the species boundaries and phylogenetic relationships of the three <i>Dolycoris</i> species distributed across the Chinese mainland. |
| first_indexed | 2024-03-07T22:28:13Z |
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| language | English |
| last_indexed | 2024-03-07T22:28:13Z |
| publishDate | 2024-02-01 |
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| series | Insects |
| spelling | doaj.art-ff57844ac336469cbca2874e64b899012024-02-23T15:21:35ZengMDPI AGInsects2075-44502024-02-0115213410.3390/insects15020134Mitogenomes Provide Insights into the Species Boundaries and Phylogenetic Relationships among Three <i>Dolycoris</i> Sloe Bugs (Hemiptera: Pentatomidae) from ChinaChenguang Zheng0Xiuxiu Zhu1Ying Wang2Xue Dong3Ruijuan Yang4Zechen Tang5Wenjun Bu6Institute of Entomology, College of Life Sciences, Nankai University, 94 Weijin Road, Tianjin 300071, ChinaInstitute of Entomology, College of Life Sciences, Nankai University, 94 Weijin Road, Tianjin 300071, ChinaInstitute of Entomology, College of Life Sciences, Nankai University, 94 Weijin Road, Tianjin 300071, ChinaInstitute of Entomology, College of Life Sciences, Nankai University, 94 Weijin Road, Tianjin 300071, ChinaInstitute of Entomology, College of Life Sciences, Nankai University, 94 Weijin Road, Tianjin 300071, ChinaInstitute of Entomology, College of Life Sciences, Nankai University, 94 Weijin Road, Tianjin 300071, ChinaInstitute of Entomology, College of Life Sciences, Nankai University, 94 Weijin Road, Tianjin 300071, China(1) Background: The three sloe bugs, <i>Dolycoris baccarum</i>, <i>Dolycoris indicus,</i> and <i>Dolycoris penicillatus</i>, are found in the Chinese mainland and are morphologically similar. The species boundaries and phylogenetic relationships of the three species remain uncertain; (2) Methods: In this study, we generated multiple mitochondrial genomes (mitogenomes) for each of the three species and conducted comparative mitogenomic analysis, species delimitation, and phylogenetic analysis based on these data; (3) Results: Mitogenomes of the three <i>Dolycoris</i> species are conserved in nucleotide composition, gene arrangement, and codon usage. All protein-coding genes (PCGs) were found to be under purifying selection, and the ND4 evolved at the fastest rate. Most species delimitation analyses based on the COI gene and the concatenated 13 PCGs retrieved three operational taxonomic units (OTUs), which corresponded well with the three <i>Dolycoris</i> species identified based on morphological characters. A clear-cut barcode gap was discovered between the interspecific and intraspecific genetic distances of the three <i>Dolycoris</i> species. Phylogenetic analyses strongly supported the monophyly of <i>Dolycoris</i>, with interspecific relationship inferred as (<i>D. indicus</i> + (<i>D. baccarum</i> + <i>D. penicillatus</i>)); (4) Conclusions: Our study provides the first insight into the species boundaries and phylogenetic relationships of the three <i>Dolycoris</i> species distributed across the Chinese mainland.https://www.mdpi.com/2075-4450/15/2/134<i>Dolycoris</i>mitogenomespecies delimitationphylogeny |
| spellingShingle | Chenguang Zheng Xiuxiu Zhu Ying Wang Xue Dong Ruijuan Yang Zechen Tang Wenjun Bu Mitogenomes Provide Insights into the Species Boundaries and Phylogenetic Relationships among Three <i>Dolycoris</i> Sloe Bugs (Hemiptera: Pentatomidae) from China Insects <i>Dolycoris</i> mitogenome species delimitation phylogeny |
| title | Mitogenomes Provide Insights into the Species Boundaries and Phylogenetic Relationships among Three <i>Dolycoris</i> Sloe Bugs (Hemiptera: Pentatomidae) from China |
| title_full | Mitogenomes Provide Insights into the Species Boundaries and Phylogenetic Relationships among Three <i>Dolycoris</i> Sloe Bugs (Hemiptera: Pentatomidae) from China |
| title_fullStr | Mitogenomes Provide Insights into the Species Boundaries and Phylogenetic Relationships among Three <i>Dolycoris</i> Sloe Bugs (Hemiptera: Pentatomidae) from China |
| title_full_unstemmed | Mitogenomes Provide Insights into the Species Boundaries and Phylogenetic Relationships among Three <i>Dolycoris</i> Sloe Bugs (Hemiptera: Pentatomidae) from China |
| title_short | Mitogenomes Provide Insights into the Species Boundaries and Phylogenetic Relationships among Three <i>Dolycoris</i> Sloe Bugs (Hemiptera: Pentatomidae) from China |
| title_sort | mitogenomes provide insights into the species boundaries and phylogenetic relationships among three i dolycoris i sloe bugs hemiptera pentatomidae from china |
| topic | <i>Dolycoris</i> mitogenome species delimitation phylogeny |
| url | https://www.mdpi.com/2075-4450/15/2/134 |
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